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2019
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27 Downloads (Pure)
Open Access
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1 Citation (Scopus)
6 Downloads (Pure)

Genome and proteome of the chlorophyll f-producing cyanobacterium Halomicronema hongdechloris: adaptative proteomic shifts under different light conditions

Chen, M., Hernandez-Prieto, M. A., Loughlin, P. C., Li, Y. & Willows, R. D., 12 Mar 2019, In : BMC Genomics. 20, p. 1-15 15 p., 207.

Research output: Contribution to journalArticle

Open Access
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4 Citations (Scopus)
1 Downloads (Pure)

Spectral signatures of five hydroxymethyl chlorophyll a derivatives chemically derived from chlorophyll b or chlorophyll f

Sawicki, A., Willows, R. D. & Chen, M., Apr 2019, In : Photosynthesis Research. 140, 1, p. 115-127 13 p.

Research output: Contribution to journalArticle

2 Citations (Scopus)

Subsurface Stappia: success through defence, specialisation and putative pressure-dependent carbon fixation

Vick, S. H. W., Greenfield, P., Willows, R. D., Tetu, S. G., Midgley, D. J. & Paulsen, I. T., 11 Dec 2019, In : Microbial Ecology.

Research output: Contribution to journalArticle

2018

Effect of processing on Mungbean (Vigna radiata) flour nutritional properties and protein composition

Skylas, D. J., Molloy, M. P., Willows, R. D., Salman, H., Blanchard, C. L. & Quail, K. J., 2018, In : Journal of Agricultural Science. 10, 11, p. 16-28 13 p.

Research output: Contribution to journalArticle

Open Access
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67 Downloads (Pure)
2017

1-N-histidine phosphorylation of ChlD by the AAA⁺ ChlI2 stimulates magnesium chelatase activity in chlorophyll synthesis

Sawicki, A., Shuaixiang, Z., Kwiatkowski, K., Luo, M. & Willows, R., Jun 2017, In : Biochemical Journal. 474, 12, p. 2095-2105 11 p.

Research output: Contribution to journalArticle

Open Access
5 Citations (Scopus)

Characterisation of protein isolates prepared from processed mungbean (Vigna radiata) flours

Skylas, D. J., Molloy, M. P., Willows, R. D., Blanchard, C. L. & Quail, K. J., 2017, In : Journal of Agricultural Science. 9, 12, p. 1-10 10 p.

Research output: Contribution to journalArticle

Open Access
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74 Downloads (Pure)

The C21-formyl group in chlorophyll f originates from molecular oxygen

Garg, H., Loughlin, P. C., Willows, R. D. & Chen, M., 24 Nov 2017, In : Journal of Biological Chemistry. 292, 47, p. 19279-19289 11 p.

Research output: Contribution to journalArticle

11 Citations (Scopus)
2016

Characterization of red-shifted phycobilisomes isolated from the chlorophyll f-containing cyanobacterium Halomicronema hongdechloris

Li, Y., Lin, Y., Garvey, C. J., Birch, D., Corkery, R. W., Loughlin, P. C., Scheer, H., Willows, R. D. & Chen, M., 1 Jan 2016, In : Biochimica et Biophysica Acta - Bioenergetics. 1857, 1, p. 107-114 8 p.

Research output: Contribution to journalArticle

40 Citations (Scopus)

GUN4-Protoporphyrin IX Is a singlet oxygen generator with consequences for plastid retrograde signaling

Tabrizi, S. T., Sawicki, A., Zhou, S., Luo, M. & Willows, R. D., 22 Apr 2016, In : Journal of Biological Chemistry. 291, 17, p. 8978-8984 7 p.

Research output: Contribution to journalArticle

14 Citations (Scopus)

Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand

Sayyadi, N., Justiniano, I., Connally, R. E., Zhang, R., Shi, B., Kautto, L., Everest-Dass, A. V., Yuan, J., Walsh, B. J., Jin, D., Willows, R. D., Piper, J. A. & Packer, N. H., 4 Oct 2016, In : Analytical Chemistry. 88, 19, p. 9564-9571 8 p.

Research output: Contribution to journalArticle

11 Citations (Scopus)

Spectral properties of bacteriophytochrome AM1_5894 in the chlorophyll d-containing cyanobacterium Acaryochloris marina

Loughlin, P. C., Duxbury, Z., Mugerwa, T. T. M., Smith, P. M. C., Willows, R. D. & Chen, M., 10 Jun 2016, In : Scientific Reports. 6, p. 1-12 12 p., 27547.

Research output: Contribution to journalArticle

Open Access
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8 Citations (Scopus)
9 Downloads (Pure)

Stable upconversion nanohybrid particles for specific prostate cancer cell immunodetection

Shi, Y., Shi, B., Dass, A. V. E., Lu, Y., Sayyadi, N., Kautto, L., Willows, R. D., Chung, R., Piper, J., Nevalainen, H., Walsh, B., Jin, D. & Packer, N. H., 22 Nov 2016, In : Scientific Reports. 6, p. 1-11 11 p., 37533.

Research output: Contribution to journalArticle

Open Access
File
11 Citations (Scopus)
17 Downloads (Pure)
2015

Hydroxylation of the C13(2) and C18 carbons of chlorophylls by heme and molecular oxygen

Loughlin, P. C., Willows, R. D. & Chen, M., 1 Sep 2015, In : Journal of Porphyrins and Phthalocyanines. 19, 9, p. 1007-1013 7 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Structure of GUN4 from Chlamydomonas reinhardtii

Tarahi Tabrizi, S., Langley, D. B., Harrop, S. J., Duff, A. P. & Willows, R. D., 1 Aug 2015, In : Acta Crystallographica Section:F Structural Biology Communications. 71, p. 1094-1099 6 p.

Research output: Contribution to journalArticle

Open Access
File
4 Citations (Scopus)
12 Downloads (Pure)
2014

Inducing the oxidative stress response in Escherichia coli improves the quality of a recombinant protein: Magnesium chelatase ChlH

Müller, A. H., Sawicki, A., Zhou, S., Tabrizi, S. T., Luo, M., Hansson, M. & Willows, R. D., 2014, In : Protein Expression and Purification. 101, p. 61-67 7 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)

In vitro conversion of vinyl to formyl groups in naturally occurring chlorophylls

Loughlin, P. C., Willows, R. D. & Chen, M., 14 Aug 2014, In : Scientific Reports. 4, p. 1-9 9 p., 6069.

Research output: Contribution to journalArticle

Open Access
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20 Citations (Scopus)
24 Downloads (Pure)
2013

Mutation of cysteine residues alters the heme-binding pocket of indoleamine 2,3-dioxygenase-1

Austin, C. J. D., Kosim-Satyaputra, P., Smith, J. R., Willows, R. D. & Jamie, J. F., 12 Jul 2013, In : Biochemical and Biophysical Research Communications. 436, 4, p. 595-600 6 p.

Research output: Contribution to journalArticle

4 Citations (Scopus)

Structure of chlorophyll f

Willows, R. D., Li, Y., Scheer, H. & Chen, M., 5 Apr 2013, In : Organic Letters. 15, 7, p. 1588-1590 3 p.

Research output: Contribution to journalArticle

34 Citations (Scopus)
2012

A cyanobacterium that contains chlorophyll f - A red-absorbing photopigment

Chen, M., Li, Y., Birch, D. & Willows, R. D., 21 Sep 2012, In : FEBS Letters. 586, 19, p. 3249-3254 6 p.

Research output: Contribution to journalArticle

91 Citations (Scopus)

C-terminal residues of Oryza sativa GUN4 are required for the activation of the ChlH subunit of magnesium chelatase in chlorophyll synthesis

Zhou, S., Sawicki, A., Willows, R. D. & Luo, M., 3 Feb 2012, In : FEBS Letters. 586, 3, p. 205-210 6 p.

Research output: Contribution to journalArticle

27 Citations (Scopus)

Extinction coefficient for red-shifted chlorophylls: Chlorophyll d and chlorophyll f

Li, Y., Scales, N., Blankenship, R. E., Willows, R. D. & Chen, M., Aug 2012, In : Biochimica et Biophysica Acta - Bioenergetics. 1817, 8, p. 1292-1298 7 p.

Research output: Contribution to journalArticle

80 Citations (Scopus)

Novel indoleamine 2,3-dioxygenase-1 inhibitors from a multistep in silico screen

Smith, J. R., Evans, K. J., Wright, A., Willows, R. D., Jamie, J. F. & Griffith, R., 1 Feb 2012, In : Bioorganic and Medicinal Chemistry. 20, 3, p. 1354-1363 10 p.

Research output: Contribution to journalArticle

35 Citations (Scopus)
2011

Mammalian forebrain ketimine reductase identified as μ-crystallin; Potential regulation by thyroid hormones

Hallen, A., Cooper, A. J. L., Jamie, J. F., Haynes, P. A. & Willows, R. D., Aug 2011, In : Journal of Neurochemistry. 118, 3, p. 379-387 9 p.

Research output: Contribution to journalArticle

43 Citations (Scopus)

Methods for the preparation of chlorophyllide a: An intermediate of the chlorophyll biosynthetic pathway

Müller, A. H., Gough, S. P., Bollivar, D. W., Meldal, M., Willows, R. D. & Hansson, M., 15 Dec 2011, In : Analytical Biochemistry. 419, 2, p. 271-276 6 p.

Research output: Contribution to journalArticle

12 Citations (Scopus)
2010

18O labeling of chlorophyll d in Acaryochloris marina reveals that chlorophyll a and molecular oxygen are precursors

Schliep, M., Crossett, B., Willows, R. D. & Chen, M., 10 Sep 2010, In : Journal of Biological Chemistry. 285, 37, p. 28450-28456 7 p.

Research output: Contribution to journalArticle

45 Citations (Scopus)

A red-shifted chlorophyll

Chen, M., Schliep, M., Willows, R. D., Cai, Z. L., Neilan, B. A. & Scheer, H., 10 Sep 2010, In : Science. 329, 5997, p. 1318-1319 2 p.

Research output: Contribution to journalArticle

277 Citations (Scopus)

ATP-Induced Conformational Dynamics in the AAA+ Motor Unit of Magnesium Chelatase

Lundqvist, J., Elmlund, H., Wulff, R. P., Berglund, L., Elmlund, D., Emanuelsson, C., Hebert, H., Willows, R. D., Hansson, M., Lindahl, M. & Al-Karadaghi, S., 10 Mar 2010, In : Structure. 18, 3, p. 354-365 12 p.

Research output: Contribution to journalArticle

47 Citations (Scopus)

BchJ and BchM interact in a 1 1 ratio with the magnesium chelatase BchH subunit of Rhodobacter capsulatus

Sawicki, A. & Willows, R. D., Nov 2010, In : FEBS Journal. 277, 22, p. 4709-4721 13 p.

Research output: Contribution to journalArticle

19 Citations (Scopus)

Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX

Meinecke, L., Alawady, A., Schroda, M., Willows, R., Kobayashi, M. C., Niyogi, K. K., Grimm, B. & Beck, C. F., 2010, In : Plant Molecular Biology. 72, 6, p. 643-658 16 p.

Research output: Contribution to journalArticle

17 Citations (Scopus)

Strategic distribution of protective proteins within bran layers of wheat protects the nutrient-rich endosperm

Jerkovic, A., Kriegel, A. M., Bradner, J. R., Atwell, B. J., Roberts, T. H. & Willows, R. D., Mar 2010, In : Plant Physiology. 152, 3, p. 1459-1470 12 p.

Research output: Contribution to journalArticle

60 Citations (Scopus)
2009

Germination of wheat: A functional proteomics analysis of the embryo

Mak, Y., Willows, R. D., Roberts, T. H., Wrigley, C. W., Sharp, P. J. & Copeland, L., May 2009, In : Cereal Chemistry. 86, 3, p. 281-289 9 p.

Research output: Contribution to journalArticle

27 Citations (Scopus)

Mouse and human indoleamine 2,3-dioxygenase display some distinct biochemical and structural properties

Austin, C. J. D., Astelbauer, F., Kosim-Satyaputra, P., Ball, H. J., Willows, R. D., Jamie, J. F. & Hunt, N. H., Jan 2009, In : Amino Acids. 36, 1, p. 99-106 8 p.

Research output: Contribution to journalArticle

31 Citations (Scopus)
10 Citations (Scopus)
2008

Kinetic analyses of the magnesium chelatase provide insights into the mechanism, structure, and formation of the complex

Sawicki, A. & Willows, R. D., 14 Nov 2008, In : Journal of Biological Chemistry. 283, 46, p. 31294-31302 9 p.

Research output: Contribution to journalArticle

16 Citations (Scopus)

Substrate-binding model of the chlorophyll biosynthetic magnesium chelatase BchH subunit

Sirijovski, N., Lundqvist, J., Rosenbäck, M., Elmlund, H., Al-Karadaghi, S., Willows, R. D. & Hansson, M., 25 Apr 2008, In : Journal of Biological Chemistry. 283, 17, p. 11652-11660 9 p.

Research output: Contribution to journalArticle

32 Citations (Scopus)
2007

Proteome mapping of the Trichoderma reesei 20S proteasome

Grinyer, J., Kautto, L., Traini, M., Willows, R. D., Te'o, J., Bergquist, P. & Nevalainen, H., Feb 2007, In : Current Genetics. 51, 2, p. 79-88 10 p.

Research output: Contribution to journalArticle

24 Citations (Scopus)
6 Citations (Scopus)

The Chlamydomonas genome reveals the evolution of key animal and plant functions

Merchant, S. S., Prochnik, S. E., Vallon, O., Harris, E. H., Karpowicz, S. J., Witman, G. B., Terry, A., Salamov, A., Fritz-Laylin, L. K., Maréchal-Drouard, L., Marshall, W. F., Qu, L. H., Nelson, D. R., Sanderfoot, A. A., Spalding, M. H., Kapitonov, V. V., Ren, Q., Ferris, P., Lindquist, E., Shapiro, H. & 97 others, Lucas, S. M., Grimwood, J., Schmutz, J., Grigoriev, I. V., Rokhsar, D. S., Grossman, A. R., Cardol, P., Cerutti, H., Chanfreau, G., Chen, C. L., Cognat, V., Croft, M. T., Dent, R., Dutcher, S., Fernández, E., Fukuzawa, H., González-Ballester, D., González-Halphen, D., Hallmann, A., Hanikenne, M., Hippler, M., Inwood, W., Jabbari, K., Kalanon, M., Kuras, R., Lefebvre, P. A., Lemaire, S. D., Lobanov, A. V., Lohr, M., Manuell, A., Meier, I., Mets, L., Mittag, M., Mittelmeier, T., Moroney, J. V., Moseley, J., Napoli, C., Nedelcu, A. M., Niyogi, K., Novoselov, S. V., Paulsen, I. T., Pazour, G., Purton, S., Ral, J. P., Riaño-Pachón, D. M., Riekhof, W., Rymarquis, L., Schroda, M., Stern, D., Umen, J., Willows, R., Wilson, N., Zimmer, S. L., Allmer, J., Balk, J., Bisova, K., Chen, C. J., Elias, M., Gendler, K., Hauser, C., Lamb, M. R., Ledford, H., Long, J. C., Minagawa, J., Page, M. D., Pan, J., Pootakham, W., Roje, S., Rose, A., Stahlberg, E., Terauchi, A. M., Yang, P., Ball, S., Bowler, C., Dieckmann, C. L., Gladyshev, V. N., Green, P., Jorgensen, R., Mayfield, S., Mueller-Roeber, B., Rajamani, S., Sayre, R. T., Brokstein, P., Dubchak, I., Goodstein, D., Hornick, L., Huang, Y. W., Jhaveri, J., Luo, Y., Martínez, D., Ngau, W. C. A., Otillar, B., Poliakov, A., Porter, A., Szajkowski, L., Werner, G. & Zhou, K., 12 Oct 2007, In : Science. 318, 5848, p. 245-251 7 p.

Research output: Contribution to journalArticle

1600 Citations (Scopus)
2006

A proteomic approach to the identification and characterisation of protein composition in wheat germ

Mak, Y., Skylas, D. J., Willows, R., Connolly, A., Cordwell, S. J., Wrigley, C. W., Sharp, P. J. & Copeland, L., Oct 2006, In : Functional and Integrative Genomics. 6, 4, p. 322-337 16 p.

Research output: Contribution to journalArticle

28 Citations (Scopus)

ATPase activity associated with the magnesium chelatase H-subunit of the chlorophyll biosynthetic pathway is an artefact

Sirijovski, N., Olsson, U., Lundqvist, J., Al-Karadaghi, S., Willows, R. D. & Hansson, M., 15 Dec 2006, In : Biochemical Journal. 400, 3, p. 477-484 8 p.

Research output: Contribution to journalArticle

15 Citations (Scopus)

Black Point is associated with reduced levels of stress, disease- and defence-related proteins in wheat grain

Mak, Y., Willows, R. D., Roberts, T. H., Wrigley, C. W., Sharp, P. J. & Copeland, L., May 2006, In : Molecular Plant Pathology. 7, 3, p. 177-189 13 p.

Research output: Contribution to journalArticle

23 Citations (Scopus)

Inhibition of indoleamine 2,3 dioxygenase activity by H2O2

Poljak, A., Grant, R., Austin, C. J. D., Jamie, J. F., Willows, R. D., Takikawa, O., Littlejohn, T. K., Truscott, R. J. W., Walker, M. J., Sachdev, P. & Smythe, G. A., 1 Jun 2006, In : Archives of Biochemistry and Biophysics. 450, 1, p. 9-19 11 p.

Research output: Contribution to journalArticle

23 Citations (Scopus)

Recessiveness and dominance in barley mutants deficient in Mg-chelatase subunit D, an AAA protein involved in chlorophyll biosynthesis

Axelsson, E., Lundqvist, J., Sawicki, A., Nilsson, S., Schröder, I., Al-Karadaghi, S., Willows, R. D. & Hansson, M., Dec 2006, In : Plant Cell. 18, 12, p. 3606-3616 11 p.

Research output: Contribution to journalArticle

38 Citations (Scopus)
2005

The Chlamydomonas reinhardtii gtr gene encoding the tetrapyrrole biosynthetic enzyme glutamyl-tRNA reductase: Structure of the gene and properties of the expressed enzyme

Srivastava, A., Lake, V., Nogaj, L. A., Mayer, S. M., Willows, R. D. & Beale, S. I., Jul 2005, In : Plant Molecular Biology. 58, 5, p. 643-658 16 p.

Research output: Contribution to journalArticle

19 Citations (Scopus)
2004

ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo

Lake, V., Obson, U., Willows, R. D. & Hansson, M., Jun 2004, In : European Journal of Biochemistry. 271, 11, p. 2182-2188 7 p.

Research output: Contribution to journalArticle

26 Citations (Scopus)

EM single particle analysis of the ATP-dependent BchI complex of magnesium chelatase: An AAA+ hexamer

Willows, R. D., Hansson, A., Birch, D., Al-Karadaghi, S. & Hansson, M., Apr 2004, In : Journal of Structural Biology. 146, 1-2, p. 227-233 7 p.

Research output: Contribution to journalArticle

37 Citations (Scopus)

New enzymes from environmental cassette arrays: functional attributes of a phosphotransferase and an RNA-methyltransferase

Nield, B. S., Willows, R. D., Torda, A. E., Gillings, M. R., Holmes, A. J., Nevalainen, K. M. H., Stokes, H. W. & Mabbutt, B. C., Jun 2004, In : Protein Science. 13, 6, p. 1651-1659 9 p.

Research output: Contribution to journalArticle

21 Citations (Scopus)

Optimised expression and purification of recombinant human indoleamine 2,3-dioxygenase

Austin, C. J. D., Mizdrak, J., Matin, A., Sirijovski, N., Kosim-Satyaputra, P., Willows, R. D., Robert, T. H., Truscott, R. J. W., Polekhina, G., Parker, M. W. & Jamie, J. F., Oct 2004, In : Protein Expression and Purification. 37, 2, p. 392-398 7 p.

Research output: Contribution to journalArticle

32 Citations (Scopus)