Sakkie Pretorius

Professor

  • 12100 Citations
  • 54 h-Index
1983 …2020
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Research Outputs 1983 2019

2019
Open Access
File
Benchmarking
Metabolism
Yeast
Methanol
Saccharomyces cerevisiae

Building a global alliance of biofoundries

Hillson, N. J., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 othersMenolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., 9 May 2019, In : Nature Communications. 10, 10, p. 1-4 4 p., 2040.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
biotechnology
Biotechnology
organisms
Testing
Research

Evolutionary engineering in Saccharomyces cerevisiae reveals a TRK1-dependent potassium influx mechanism for propionic acid tolerance

Xu, X., Williams, T. C., Divne, C., Pretorius, I. S. & Paulsen, I. T., 23 Apr 2019, In : Biotechnology for Biofuels. 12, 14 p., 97.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Propionic acid
Yeast
Saccharomyces cerevisiae
Potassium
potassium

Saccharomyces cerevisiae

Belda, I., Ruiz, J., Santos, A., Van Wyk, N. & Pretorius, I. S., Dec 2019, In : Trends in Genetics. 35, 12, p. 956-957 2 p.

Research output: Contribution to journalArticleResearchpeer-review

2018

Applications of yeast synthetic biology geared towards the production of biopharmaceuticals

Walker, R. S. K. & Pretorius, I. S., Jul 2018, In : Genes. 9, 7, p. 1-22 22 p., 340.

Research output: Contribution to journalReview articleResearchpeer-review

Open Access
File
Synthetic Biology
Yeasts
Metabolic Networks and Pathways

Population sequencing reveals clonal diversity and ancestral inbreeding in the grapevine cultivar Chardonnay

Roach, M. J., Johnson, D. L., Bohlmann, J., van Vuuren, H. J. J., Jones, S. J. M., Pretorius, I. S., Schmidt, S. A. & Borneman, A. R., 20 Nov 2018, In : PLoS Genetics. 14, 11, p. 1-24 24 p., e1007807.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Inbreeding
inbreeding
cultivar
genome
Genome

Systems-based approaches enable identification of gene targets which improve the flavour profile of low-ethanol wine yeast strains

Varela, C., Schmidt, S. A., Borneman, A. R., Pang, C. N. I., Krömerx, J. O., Khan, A., Song, X., Hodson, M. P., Solomon, M., Mayr, C. M., Hines, W., Pretorius, I. S., Baker, M. S., Roessner, U., Mercurio, M., Henschke, P. A., Wilkins, M. R. & Chambers, P. J., 1 Sep 2018, In : Metabolic Engineering. 49, p. 178-191 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Flavors
Yeast
Ethanol
Genes

The smell of synthetic biology: engineering strategies for aroma compound production in yeast

van Wyk, N., Kroukamp, H. & Pretorius, I. S., Sep 2018, In : Fermentation. 4, 3, p. 1-18 18 p., 54.

Research output: Contribution to journalReview articleResearchpeer-review

Open Access
File
synthetic biology
smell
odor compounds
engineering
yeasts

Yeast 2.0—connecting the dots in the construction of the world's first functional synthetic eukaryotic genome

Pretorius, I. S. & Boeke, J. D., 1 Jun 2018, In : FEMS Yeast Research. 18, 4, p. 1-15 15 p., foy032.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Synthetic Biology
Yeasts
Genome
Technology
Planets
2017

Positive-feedback, ratiometric biosensor expression improves high-throughput metabolite-producer screening efficiency in yeast

Williams, T. C., Xu, X., Ostrowski, M., Pretorius, I. S. & Paulsen, I. T., 29 Jan 2017, In : Synthetic Biology. 2, 1, p. 1-13 13 p., 1.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Biosensing Techniques
Green Fluorescent Proteins
Yeasts
Synthetic Biology
Transcription Factors

Solving yeast jigsaw puzzles over a glass of wine: Synthetic genome engineering pioneers new possibilities for wine yeast research

Pretorius, I. S., 1 Nov 2017, In : EMBO Reports. 18, 11, p. 1875-1884 10 p.

Research output: Contribution to journalComment/opinionResearch

Open Access
File

Synthetic biology stretching the realms of possibility in wine yeast research

Jagtap, U. B., Jadhav, J. P., Bapat, V. A. & Pretorius, I. S., 3 Jul 2017, In : International Journal of Food Microbiology. 252, p. 24-34 11 p.

Research output: Contribution to journalReview articleResearchpeer-review

Synthetic Biology
synthetic biology
wine yeasts
Wine
Yeast

Synthetic genome engineering forging new frontiers for wine yeast

Pretorius, I. S., 2 Jan 2017, In : Critical Reviews in Biotechnology. 37, 1, p. 112-136 25 p.

Research output: Contribution to journalReview articleResearchpeer-review

Wine
Yeasts
Genome
Synthetic Biology
Industry

Yeast's balancing act between ethanol and glycerol production in low-alcohol wines

Goold, H. D., Kroukamp, H., Williams, T. C., Paulsen, I. T., Varela, C. & Pretorius, I. S., 1 Mar 2017, In : Microbial Biotechnology. 10, 2, p. 264-278 15 p.

Research output: Contribution to journalReview articleResearchpeer-review

Open Access
File
Wine
Glycerol
Yeast
Alcohols
Ethanol
2016

Heterologous production of raspberry ketone in the wine yeast Saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion

Lee, D., Lloyd, N. D. R., Pretorius, I. S. & Borneman, A. R., 4 Mar 2016, In : Microbial Cell Factories. 15, p. 1-7 7 p., 49.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Wine
Ketones
Yeast
Saccharomyces cerevisiae
Fusion reactions

Synthetic Evolution of Metabolic Productivity Using Biosensors

Williams, T. C., Pretorius, I. S. & Paulsen, I. T., 1 May 2016, In : Trends in Biotechnology. 34, 5, p. 371-381 11 p.

Research output: Contribution to journalReview articleResearchpeer-review

Synthetic Biology
Biosensing Techniques
Biological systems
Biosensors
Systems Biology
2015

Designing wine yeast for the future

Pretorius, I. S., Curtin, C. D. & Chambers, P. J., 2015, Advances in Fermented Foods and Beverages: Improving Quality, Technologies and Health Benefits. Holzapfel, W. (ed.). Cambridge: Woodhead Publishing, p. 197-226 30 p. (Woodhead Publishing Series in Food Science, Technology and Nutrition; vol. 265).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

wine yeasts
Wine
Yeast
Yeasts
winemaking

Elucidating whole-genome design principles using synthetic genomics: The Yeast 2.0 project

Williams, T. C., Wightman, E. L. I., Daniel, E. L., Kroukamp, H., Nevalainen, H. K. M., Paulsen, I. T. & Pretorius, I. S., 2015, Proceedings of the 6th Australasian Conference on Yeast: Products and Discovery. Grbin, P. R. & Chambers, P. (eds.). 1 p. 3-1

Research output: Chapter in Book/Report/Conference proceedingConference abstractResearch

Genomic insights into the Saccharomyces sensu stricto complex

Borneman, A. R. & Pretorius, I. S., 1 Feb 2015, In : Genetics. 199, 2, p. 281-291 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
Yeasts
Saccharomyces cerevisiae
Domestication

Influence of diammonium phosphate addition to fermentation on wine biologicals

Vilanova, M., Pretorius, I. S. & Henschke, P. A., 2015, Processing and Impact on Active Components in Food. Preedy, V. (ed.). London: Academic Press, p. 483-491 9 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

diammonium phosphate
Wine
Fermentation
wines
Phosphates
2014

Festive foods

Pretorius, I. S., 1 Nov 2014, In : FEMS Microbiology Letters. 360, 2, p. 85-86 2 p.

Research output: Contribution to journalComment/opinionResearchpeer-review

Food Microbiology
Food

Genomic insights into the evolution of industrial yeast species Brettanomyces bruxellensis

Curtin, C. D. & Pretorius, I. S., 1 Nov 2014, In : FEMS Yeast Research. 14, 7, p. 997-1005 9 p.

Research output: Contribution to journalReview articleResearchpeer-review

Brettanomyces
Wine
Yeasts
Saccharomyces cerevisiae
Metagenomics

Not your ordinary yeast: Non-Saccharomyces yeasts in wine production uncovered

Jolly, N. P., Varela, C. & Pretorius, I. S., 2014, In : FEMS Yeast Research. 14, 2, p. 215-237 23 p.

Research output: Contribution to journalReview articleResearchpeer-review

Wine
Yeasts
Saccharomyces cerevisiae
Vitis
Fermentation

Whole-genome comparison reveals novel genetic elements that characterize the genome of industrial strains of Saccharomyces cerevisiae

Borneman, A. R., Desany, B. A., Riches, D., Affourtit, J. P., Forgan, A. H., Pretorius, I. S., Egholm, M. & Chambers, P. J., 2014, Investigations in yeast functional genomics and molecular biology. Eckwahl, M. (ed.). Toronto: Apple Academic Press, p. 339-362 24 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Wine, beer and cider: Unravelling the aroma profile

Gamero, A., Ferreira, V., Pretorius, I. S. & Querol, A., 2014, Molecular Mechanisms in Yeast Carbon Metabolism. Piškur, J. & Compagno, C. (eds.). Berlin; Heidelberg: Springer, Springer Nature, p. 261-297 37 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Wine
Yeasts
Alcoholic Beverages
Beverages
Fermentation
2013

At the cutting-edge of grape and wine biotechnology

Borneman, A. R., Schmidt, S. A. & Pretorius, I. S., Apr 2013, In : Trends in Genetics. 29, 4, p. 263-271 9 p.

Research output: Contribution to journalReview articleResearchpeer-review

Vitis
Wine
Biotechnology
Systems Biology
Biological Science Disciplines

Comparative genomics: A revolutionary tool for wine yeast strain development

Borneman, A. R., Pretorius, I. S. & Chambers, P. J., Apr 2013, In : Current Opinion in Biotechnology. 24, 2, p. 192-199 8 p.

Research output: Contribution to journalReview articleResearchpeer-review

Starters
Wine
Genomics
Yeast
Yeasts

Novel wine yeast with mutations in YAP1 that produce less acetic acid during fermentation

Cordente, A. G., Cordero-Bueso, G., Pretorius, I. S. & Curtin, C. D., Jan 2013, In : FEMS Yeast Research. 13, 1, p. 62-73 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Acetic Acid
Cerulenin
Fermentation
Yeasts
2012

A breeding strategy to harness flavor diversity of Saccharomyces interspecific hybrids and minimize hydrogen sulfide production

Bizaj, E., Cordente, A. G., Bellon, J. R., Raspor, P., Curtin, C. D. & Pretorius, I. S., Jun 2012, In : FEMS Yeast Research. 12, 4, p. 456-465 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Hydrogen Sulfide
Saccharomyces
Wine
Breeding
Yeasts

Controlling the highs and the lows of alcohol in wine

Stockley, C. S., Varela, C., Coulter, A., Dry, P. R., Francis, I. L., Muhlack, R. & Pretorius, I. S., Aug 2012, Wine: Types, Production and Health. Peeters, A. S. (ed.). New York: Nova Science Publishers, p. 281-299 19 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Wine
wines
alcohols
Alcohols
winemaking

De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast dekkera bruxellensis AWRI1499

Curtin, C. D., Borneman, A. R., Chambers, P. J. & Pretorius, I. S., 28 Mar 2012, In : PLoS ONE. 7, 3, p. 1-10 10 p., e33840.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Dekkera
Spoilage
Triploidy
Wine
triploidy

Effect of ammonium nitrogen supplementation of grape juice on wine volatiles and non-volatiles composition of the aromatic grape variety Albarino

Vilanova, M., Siebert, T. E., Varela, C., Pretorius, I. S. & Henschke, P. A., 1 Jul 2012, In : Food Chemistry. 133, 1, p. 124-131 8 p.

Research output: Contribution to journalArticleResearchpeer-review

grape juice
ammonium nitrogen
Wine
Vitis
Ammonium Compounds

Evaluation of gene modification strategies for the development of low-alcohol-wine Yeasts

Varela, C., Kutyna, D. R., Solomon, M. R., Black, C. A., Borneman, A., Henschke, P. A., Pretorius, I. S. & Chambers, P. J., Sep 2012, In : Applied and Environmental Microbiology. 78, 17, p. 6068-6077 10 p.

Research output: Contribution to journalArticleResearchpeer-review

wine yeasts
Wine
yeast
alcohol
ethanol

Flavour-active wine yeasts

Cordente, A. G., Curtin, C. D., Varela, C. & Pretorius, I. S., Nov 2012, In : Applied Microbiology and Biotechnology. 96, 3, p. 601-618 18 p.

Research output: Contribution to journalReview articleResearchpeer-review

Open Access
File
Wine
Yeasts
Beverages
Vitis
Phenotype

The genome sequence of the wine yeast VIN7 reveals an allotriploid hybrid genome with Saccharomyces cerevisiae and Saccharomyces kudriavzevii origins

Borneman, A. R., Desany, B. A., Riches, D., Affourtit, J. P., Forgan, A. H., Pretorius, I. S., Egholm, M. & Chambers, P. J., Feb 2012, In : FEMS Yeast Research. 12, 1, p. 88-96 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
Wine
Saccharomyces cerevisiae
Yeasts
Genome

The Winemaker's bug: From ancient wisdom to opening new vistas with frontier yeast science

Pretorius, I. S., Curtin, C. D. & Chambers, P. J., Jul 2012, In : Bioengineered Bugs. 3, 3, p. 147-156 10 p.

Research output: Contribution to journalReview articleResearchpeer-review

Wine
Yeast
Yeasts
Research
Fermentation

Wine-Omics: new platforms for the improvement of yeast strains and wine quality

Ting, C. S. C., Borneman, A. R. & Pretorius, I. S., 2012, OMICS technologies: tools for food science. Benkeblia, N. (ed.). Boca Raton: CRC Press, Taylor & Francis Group, p. 339-365 27 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

2011

Whole-genome comparison reveals novel genetic elements that characterize the genome of industrial strains of saccharomyces cerevisiae

Borneman, A. R., Desany, B. A., Riches, D., Affourtit, J. P., Forgan, A. H., Pretorius, I. S., Egholm, M. & Chambers, P. J., 7 Feb 2011, In : PLoS Genetics. 7, 2, p. 1-10 10 p., e1001287.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Saccharomyces cerevisiae
genome
Genome
genome assembly
yeast
2010

Fermenting knowledge: The history of winemaking, science and yeast research

Chambers, P. J. & Pretorius, I. S., Dec 2010, In : EMBO Reports. 11, 12, p. 914-920 7 p.

Research output: Contribution to journalReview articleResearchpeer-review

Preliminary evaluation of infrared spectroscopy for the differentiation of Brettanomyces bruxellensis strains isolated from red wines

Oelofse, A., Malherbe, S., Pretorius, I. S. & Du Toit, M., 15 Oct 2010, In : International Journal of Food Microbiology. 143, 3, p. 136-142 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Brettanomyces
Wine
infrared spectroscopy
red wines
wines

The effect of multiple yeasts co-inoculations on Sauvignon Blanc wine aroma composition, sensory properties and consumer preference

King, E. S., Kievit, R. L., Curtin, C., Swiegers, J. H., Pretorius, I. S., Bastian, S. E. P. & Leigh Francis, I., 1 Oct 2010, In : Food Chemistry. 122, 3, p. 618-626 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
consumer preferences
Yeast
wines
sensory properties

Wine fermentation

Curtin, C., Chambers, P. & Pretorius, S., 2010, Encyclopedia of biotechnology in agriculture and food. Heldman, D. R., Wheeler, M. B. & Hoover, D. G. (eds.). London: Taylor & Francis, p. 689-694 6 p.

Research output: Chapter in Book/Report/Conference proceedingEntry for encyclopedia/dictionary/reference bookResearch

biotechnology
modernization
fermentation
twenty first century
science and technology
2009

Differential synthesis of fermentative aroma compounds of two related commercial wine yeast strains

Molina, A. M., Guadalupe, V., Varela, C., Swiegers, J. H., Pretorius, I. S. & Agosin, E., 15 Nov 2009, In : Food Chemistry. 117, 2, p. 189-195 7 p.

Research output: Contribution to journalArticleResearchpeer-review

wine yeasts
Wine
Flavors
odor compounds
Yeast

Establishing a risk-assessment process for release of genetically modified wine yeast into the environment

Schoeman, H., Wolfaardt, G. M., Botha, A., Van Rensburg, P. & Pretorius, I. S., Aug 2009, In : Canadian Journal of Microbiology. 55, 8, p. 990-1002 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Biofilms
Yeasts
Genetically Modified Organisms
Cryptococcus

Identification of yeast population dynamics of spontaneous fermentation in Beijing wine region, China

Sun, H., Ma, H., Hao, M., Pretorius, I. S. & Chen, S., 2009, In : Annals of Microbiology. 59, 1, p. 69-76 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Population Dynamics
Wine
Fermentation
China
Yeasts

Isolation of sulfite reductase variants of a commercial wine yeast with significantly reduced hydrogen sulfide production

Cordente, A. G., Heinrich, A., Pretorius, I. S. & Swiegers, J. H., May 2009, In : FEMS Yeast Research. 9, 3, p. 446-459 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Oxidoreductases Acting on Sulfur Group Donors
Hydrogen Sulfide
Wine
Yeasts
Fermentation

Microbial formation and modification of flavor and off-flavor compounds in wine

Bartowsky, E. J. & Pretorius, I. S., 2009, Biology of microorganisms on grapes, in must and in wine. König, H., Unden, G. & Fröhlich, J. (eds.). Berlin: Springer, Springer Nature, p. 209-231 23 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

flavor compounds
off flavors
Wine
wines
flavor

Non-genetic engineering approaches for isolating and generating novel yeasts for industrial applications

Chambers, P. J., Bellon, J. R., Schmidt, S. A., Varela, C. & Pretorius, I. S., 2009, Yeast Biotechnology: Diversity and applications. Satyanarayana, T. & Kunze, G. (eds.). Dordrecht: Springer, Springer Nature, p. 433-457 25 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Yeast
Industrial applications
Genetic engineering
Biochemistry
Beverages

Systems biology as a platform for wine yeast strain development

Borneman, A. R., Chambers, P. J. & Pretorius, I. S., 2009, Biology of microorganisms on grapes, in must and in wine. König, H., Unden, G. & Fröhlich, J. (eds.). Berlin: Springer, Springer Nature, p. 395-414 20 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

wine yeasts
Systems Biology
Wine
Yeasts
Biological Sciences

The Development of Yeast Strains as Tools for Adjusting the Flavor of Fermented Beverages to Market Specifications

Swiegers, J. H., Saerens, S. M. G. & Pretorius, I. S., 2 Feb 2009, Biotechnology in Flavor Production. Havkin-Frenkel, D. & Belanger, F. C. (eds.). Oxford ; Ames, Iowa: Blackwell Publishing, p. 1-55 55 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review