Sakkie Pretorius

Professor

  • 12100 Citations
  • 54 h-Index
1983 …2020
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Research Outputs 1983 2019

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Article
2019
Open Access
File
Benchmarking
Metabolism
Yeast
Methanol
Saccharomyces cerevisiae

Building a global alliance of biofoundries

Hillson, N. J., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 othersMenolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., 9 May 2019, In : Nature Communications. 10, 10, p. 1-4 4 p., 2040.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
biotechnology
Biotechnology
organisms
Testing
Research

Evolutionary engineering in Saccharomyces cerevisiae reveals a TRK1-dependent potassium influx mechanism for propionic acid tolerance

Xu, X., Williams, T. C., Divne, C., Pretorius, I. S. & Paulsen, I. T., 23 Apr 2019, In : Biotechnology for Biofuels. 12, 14 p., 97.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Propionic acid
Yeast
Saccharomyces cerevisiae
Potassium
potassium

Saccharomyces cerevisiae

Belda, I., Ruiz, J., Santos, A., Van Wyk, N. & Pretorius, I. S., Dec 2019, In : Trends in Genetics. 35, 12, p. 956-957 2 p.

Research output: Contribution to journalArticleResearchpeer-review

2018

Population sequencing reveals clonal diversity and ancestral inbreeding in the grapevine cultivar Chardonnay

Roach, M. J., Johnson, D. L., Bohlmann, J., van Vuuren, H. J. J., Jones, S. J. M., Pretorius, I. S., Schmidt, S. A. & Borneman, A. R., 20 Nov 2018, In : PLoS Genetics. 14, 11, p. 1-24 24 p., e1007807.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Inbreeding
inbreeding
cultivar
genome
Genome

Systems-based approaches enable identification of gene targets which improve the flavour profile of low-ethanol wine yeast strains

Varela, C., Schmidt, S. A., Borneman, A. R., Pang, C. N. I., Krömerx, J. O., Khan, A., Song, X., Hodson, M. P., Solomon, M., Mayr, C. M., Hines, W., Pretorius, I. S., Baker, M. S., Roessner, U., Mercurio, M., Henschke, P. A., Wilkins, M. R. & Chambers, P. J., 1 Sep 2018, In : Metabolic Engineering. 49, p. 178-191 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Flavors
Yeast
Ethanol
Genes

Yeast 2.0—connecting the dots in the construction of the world's first functional synthetic eukaryotic genome

Pretorius, I. S. & Boeke, J. D., 1 Jun 2018, In : FEMS Yeast Research. 18, 4, p. 1-15 15 p., foy032.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Synthetic Biology
Yeasts
Genome
Technology
Planets
2017

Positive-feedback, ratiometric biosensor expression improves high-throughput metabolite-producer screening efficiency in yeast

Williams, T. C., Xu, X., Ostrowski, M., Pretorius, I. S. & Paulsen, I. T., 29 Jan 2017, In : Synthetic Biology. 2, 1, p. 1-13 13 p., 1.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Biosensing Techniques
Green Fluorescent Proteins
Yeasts
Synthetic Biology
Transcription Factors
2016

Heterologous production of raspberry ketone in the wine yeast Saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion

Lee, D., Lloyd, N. D. R., Pretorius, I. S. & Borneman, A. R., 4 Mar 2016, In : Microbial Cell Factories. 15, p. 1-7 7 p., 49.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Wine
Ketones
Yeast
Saccharomyces cerevisiae
Fusion reactions
2015

Genomic insights into the Saccharomyces sensu stricto complex

Borneman, A. R. & Pretorius, I. S., 1 Feb 2015, In : Genetics. 199, 2, p. 281-291 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
Yeasts
Saccharomyces cerevisiae
Domestication
2013

Novel wine yeast with mutations in YAP1 that produce less acetic acid during fermentation

Cordente, A. G., Cordero-Bueso, G., Pretorius, I. S. & Curtin, C. D., Jan 2013, In : FEMS Yeast Research. 13, 1, p. 62-73 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Acetic Acid
Cerulenin
Fermentation
Yeasts
2012

A breeding strategy to harness flavor diversity of Saccharomyces interspecific hybrids and minimize hydrogen sulfide production

Bizaj, E., Cordente, A. G., Bellon, J. R., Raspor, P., Curtin, C. D. & Pretorius, I. S., Jun 2012, In : FEMS Yeast Research. 12, 4, p. 456-465 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Hydrogen Sulfide
Saccharomyces
Wine
Breeding
Yeasts

De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast dekkera bruxellensis AWRI1499

Curtin, C. D., Borneman, A. R., Chambers, P. J. & Pretorius, I. S., 28 Mar 2012, In : PLoS ONE. 7, 3, p. 1-10 10 p., e33840.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Dekkera
Spoilage
Triploidy
Wine
triploidy

Effect of ammonium nitrogen supplementation of grape juice on wine volatiles and non-volatiles composition of the aromatic grape variety Albarino

Vilanova, M., Siebert, T. E., Varela, C., Pretorius, I. S. & Henschke, P. A., 1 Jul 2012, In : Food Chemistry. 133, 1, p. 124-131 8 p.

Research output: Contribution to journalArticleResearchpeer-review

grape juice
ammonium nitrogen
Wine
Vitis
Ammonium Compounds

Evaluation of gene modification strategies for the development of low-alcohol-wine Yeasts

Varela, C., Kutyna, D. R., Solomon, M. R., Black, C. A., Borneman, A., Henschke, P. A., Pretorius, I. S. & Chambers, P. J., Sep 2012, In : Applied and Environmental Microbiology. 78, 17, p. 6068-6077 10 p.

Research output: Contribution to journalArticleResearchpeer-review

wine yeasts
Wine
yeast
alcohol
ethanol

The genome sequence of the wine yeast VIN7 reveals an allotriploid hybrid genome with Saccharomyces cerevisiae and Saccharomyces kudriavzevii origins

Borneman, A. R., Desany, B. A., Riches, D., Affourtit, J. P., Forgan, A. H., Pretorius, I. S., Egholm, M. & Chambers, P. J., Feb 2012, In : FEMS Yeast Research. 12, 1, p. 88-96 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
Wine
Saccharomyces cerevisiae
Yeasts
Genome
2011

Whole-genome comparison reveals novel genetic elements that characterize the genome of industrial strains of saccharomyces cerevisiae

Borneman, A. R., Desany, B. A., Riches, D., Affourtit, J. P., Forgan, A. H., Pretorius, I. S., Egholm, M. & Chambers, P. J., 7 Feb 2011, In : PLoS Genetics. 7, 2, p. 1-10 10 p., e1001287.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Saccharomyces cerevisiae
genome
Genome
genome assembly
yeast
2010

Preliminary evaluation of infrared spectroscopy for the differentiation of Brettanomyces bruxellensis strains isolated from red wines

Oelofse, A., Malherbe, S., Pretorius, I. S. & Du Toit, M., 15 Oct 2010, In : International Journal of Food Microbiology. 143, 3, p. 136-142 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Brettanomyces
Wine
infrared spectroscopy
red wines
wines

The effect of multiple yeasts co-inoculations on Sauvignon Blanc wine aroma composition, sensory properties and consumer preference

King, E. S., Kievit, R. L., Curtin, C., Swiegers, J. H., Pretorius, I. S., Bastian, S. E. P. & Leigh Francis, I., 1 Oct 2010, In : Food Chemistry. 122, 3, p. 618-626 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
consumer preferences
Yeast
wines
sensory properties
2009

Differential synthesis of fermentative aroma compounds of two related commercial wine yeast strains

Molina, A. M., Guadalupe, V., Varela, C., Swiegers, J. H., Pretorius, I. S. & Agosin, E., 15 Nov 2009, In : Food Chemistry. 117, 2, p. 189-195 7 p.

Research output: Contribution to journalArticleResearchpeer-review

wine yeasts
Wine
Flavors
odor compounds
Yeast

Establishing a risk-assessment process for release of genetically modified wine yeast into the environment

Schoeman, H., Wolfaardt, G. M., Botha, A., Van Rensburg, P. & Pretorius, I. S., Aug 2009, In : Canadian Journal of Microbiology. 55, 8, p. 990-1002 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Biofilms
Yeasts
Genetically Modified Organisms
Cryptococcus

Identification of yeast population dynamics of spontaneous fermentation in Beijing wine region, China

Sun, H., Ma, H., Hao, M., Pretorius, I. S. & Chen, S., 2009, In : Annals of Microbiology. 59, 1, p. 69-76 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Population Dynamics
Wine
Fermentation
China
Yeasts

Isolation of sulfite reductase variants of a commercial wine yeast with significantly reduced hydrogen sulfide production

Cordente, A. G., Heinrich, A., Pretorius, I. S. & Swiegers, J. H., May 2009, In : FEMS Yeast Research. 9, 3, p. 446-459 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Oxidoreductases Acting on Sulfur Group Donors
Hydrogen Sulfide
Wine
Yeasts
Fermentation

The influence of yeast on the aroma of Sauvignon Blanc wine

Swiegers, J. H., Kievit, R. L., Siebert, T., Lattey, K. A., Bramley, B. R., Francis, I. L., King, E. S. & Pretorius, I. S., Apr 2009, In : Food Microbiology. 26, 2, p. 204-211 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
wines
Yeasts
odors
yeasts
2008

Coinoculated fermentations using Saccharomyces yeasts affect the volatile composition and sensory properties of Vitis vinifera L. cv. Sauvignon Blanc wines

King, E. S., Swiegers, J. H., Travis, B., Francis, I. L., Bastian, S. E. P. & Pretorius, I. S., 26 Nov 2008, In : Journal of Agricultural and Food Chemistry. 56, 22, p. 10829-10837 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
Wine
Vitis
Vitis vinifera
Yeast

Comparative genome analysis of a Saccharomyces cerevisiae wine strain

Borneman, A. R., Forgan, A. H., Pretorius, I. S. & Chambers, P. J., Nov 2008, In : FEMS Yeast Research. 8, 7, p. 1185-1195 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Saccharomyces cerevisiae
Genome
Single Nucleotide Polymorphism
Ethanol

Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae

Govender, P., Domingo, J. L., Bester, M. C., Pretorius, I. S. & Bauer, F. F., Oct 2008, In : Applied and Environmental Microbiology. 74, 19, p. 6041-6052 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Flocculation
Dominant Genes
flocculation
Fermentation
fermentation

Correlation between glucose/fructose discrepancy and hexokinase kinetic properties in different Saccharomyces cerevisiae wine yeast strains

Berthels, N. J., Cordero Otero, R. R., Bauer, F. F., Pretorius, I. S. & Thevelein, J. M., Jan 2008, In : Applied Microbiology and Biotechnology. 77, 5, p. 1083-1091 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Fructose
Hexokinase
Wine
Yeast
Glucose

Significance of Brettanomyces and Dekkera during Winemaking: A Synoptic Review

Oelofse, A., Pretorius, I. S. & du Toit, M., 2008, In : South African Journal of Enology and Viticulture. 29, 2, p. 128-144 17 p.

Research output: Contribution to journalArticleResearchpeer-review

Dekkera
Brettanomyces
winemaking
Wine
spoilage

The effect of flocculation on the efficiency of raw-starch fermentation by Saccharomyces cerevisiae producing the Lipomyces kononenkoae LKA1-encoded α-amylase

Ramachandran, N., Joubert, L., Gundlapalli, S. B., Cordero Otero, R. R. & Pretorius, I. S., 2008, In : Annals of Microbiology. 58, 1, p. 99-108 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Flocculation
alpha-Amylases
Starch
Fermentation

The Thr505 and Ser557 residues of the AGT1-encoded α-glucoside transporter are critical for maltotriose transport in Saccharomyces cerevisiae

Smit, A., Moses, S. G., Pretorius, I. S. & Cordero Otero, R. R., Apr 2008, In : Journal of Applied Microbiology. 104, 4, p. 1103-1111 9 p.

Research output: Contribution to journalArticleResearchpeer-review

maltotriose
Glucosides
glucosides
Saccharomyces cerevisiae
transporters
2007

A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation

Thanvanthri Gururajan, V., Gorwa-Grauslund, M. F., Hahn-Hägerdal, B., Pretorius, I. S. & Cordero Otero, R. R., 2007, In : Annals of Microbiology. 57, 1, p. 85-92 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Catabolite Repression
Xylose
Fermentation
Saccharomyces cerevisiae
Glucose

Assimilable nitrogen utilisation and production of volatile and non-volatile compounds in chemically defined medium by Saccharomyces cerevisiae wine yeasts

Vilanova, M., Ugliano, M., Varela, C., Siebert, T., Pretorius, I. S. & Henschke, P. A., Nov 2007, In : Applied Microbiology and Biotechnology. 77, 1, p. 145-157 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Yeast
Saccharomyces cerevisiae
Nitrogen
Yeasts

Development and characterisation of a recombinant Saccharomyces cerevisiae mutant strain with enhanced xylose fermentation properties

Thanvanthri Gururajan, V., Van Rensburg, P., Hahn-Hägerdal, B., Pretorius, I. S. & Cordero Otero, R. R., 2007, In : Annals of Microbiology. 57, 4, p. 599-607 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Xylose
Fermentation
Saccharomyces cerevisiae
Genes
D-Xylulose Reductase

Differences among AGT1-encoded α-glucoside transporters and their ability to transport maltotriose in Saccharomyces yeasts

Smit, A., Cordero Otero, R. R. & Pretorius, I. S., 2007, In : Annals of Microbiology. 57, 1, p. 77-84 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
Glucosides
Yeasts
Maltose
Membrane Transport Proteins

Effect of the cellulose-binding domain on the catalytic activity of a β-glucosidase from Saccharomycopsis fibuligera

Gundllapalli, S. B., Pretorius, I. S. & Cordero Otero, R. R., Jun 2007, In : Journal of Industrial Microbiology and Biotechnology. 34, 6, p. 413-421 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomycopsis
beta-Glucosidase
Cellulose
Catalyst activity
Catalytic Domain

Engineering volatile thiol release in Saccharomyces cerevisiae for improved wine aroma

Swiegers, J. H., Capone, D. L., Pardon, K. H., Elsey, G. M., Sefton, M. A., Francis, I. L. & Pretorius, I. S., Jul 2007, In : Yeast. 24, 7, p. 561-574 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
thiols
Sulfhydryl Compounds
Yeast
Saccharomyces cerevisiae

Influence of wine fermentation temperature on the synthesis of yeast-derived volatile aroma compounds

Molina, A. M., Swiegers, J. H., Varela, C., Pretorius, I. S. & Agosin, E., Nov 2007, In : Applied Microbiology and Biotechnology. 77, 3, p. 675-687 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Yeast
Fermentation
Yeasts
Temperature

Modulating aroma compounds during wine fermentation by manipulating carnitine acetyltransferases in Saccharomyces cerevisiae

Cordente, A. G., Swiegers, J. H., Hegardt, F. G. & Pretorius, I. S., Feb 2007, In : FEMS Microbiology Letters. 267, 2, p. 159-166 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Carnitine O-Acetyltransferase
Wine
Fermentation
Saccharomyces cerevisiae
Esters

Molecular cloning and functional expression of a novel Neurospora crassa xylose reductase in Saccharomyces cerevisiae in the development of a xylose fermenting strain

Thanvanthri Gururajan, V., Pretorius, I. S. & Cordero Otero, R. R., 2007, In : Annals of Microbiology. 57, 2, p. 223-231 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Aldehyde Reductase
Neurospora crassa
Xylose
Molecular Cloning
Saccharomyces cerevisiae

The heterologous expression of polysaccharidase-encoding genes with oenological relevance in Saccharomyces cerevisiae

Van Rensburg, P., Strauss, M. L. A., Lambrechts, M. G., Cordero Otero, R. R. & Pretorius, I. S., Dec 2007, In : Journal of Applied Microbiology. 103, 6, p. 2248-2257 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Saccharomyces cerevisiae
wines
xylanases
polygalacturonase
2006

Development of a screening method for the identification of a novel Saccharomyces cerevisiae mutant over-expressing Trichoderma reesei cellobiohydrolase II

Gundllapalli, S. B., Otero, R. R. C. & Pretorius, I. S., 2006, In : Annals of Microbiology. 56, 2, p. 143-150 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Cellulose 1,4-beta-Cellobiosidase
Trichoderma
Saccharomyces cerevisiae
Enzymes
Cellulose

Modulation of volatile thiol and ester aromas by modified wine yeast

Swiegers, J. H., Willmott, R., Hill-Ling, A., Capone, D. L., Pardon, K. H., Elsey, G. M., Howell, K. S., de Barros Lopes, M. A., Sefton, M. A., Lilly, M. & Pretorius, I. S., 2006, In : Developments in Food Science. 43, C, p. 113-116 4 p.

Research output: Contribution to journalArticleResearchpeer-review

wine yeasts
Wine
thiols
Sulfhydryl Compounds
Esters

Rapid screening of the fermentation profiles of wine yeasts by Fourier transform infrared spectroscopy

Nieuwoudt, H. H., Pretorius, I. S., Bauer, F. F., Nel, D. G. & Prior, B. A., Nov 2006, In : Journal of Microbiological Methods. 67, 2, p. 248-256 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Fourier Transform Infrared Spectroscopy
Wine
Fermentation
Yeasts
Glycerol

Regulation of respiratory growth by Ras: The glyoxylate cycle mutant, cit2Δ, is suppressed by RAS2

Swiegers, J. H., Pretorius, I. S. & Bauer, F. F., Sep 2006, In : Current Genetics. 50, 3, p. 161-171 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Citrate (si)-Synthase
Growth
Carbon
Citric Acid
Biomass

The effect of increased branched-chain amino acid transaminase activity in yeast on the production of higher alcohols and on the flavour profiles of wine and distillates

Lilly, M., Bauer, F. F., Styger, G., Lambrechts, M. G. & Pretorius, I. S., Aug 2006, In : FEMS Yeast Research. 6, 5, p. 726-743 18 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Fermentation
Keto Acids
Yeasts
Alcohols

The effect of increased yeast alchohol acetyltransferase and esterase activity on the flavour profiles of wine and distillates

Lilly, M., Bauer, F. F., Lambrechts, M. G., Swiegers, J. H., Cozzolino, D. & Pretorius, I. S., 15 Jul 2006, In : Yeast. 23, 9, p. 641-659 19 p.

Research output: Contribution to journalArticleResearchpeer-review

distillates
acetyltransferases
Acetyltransferases
Wine
Flavors

The effect of polysaccharide-degrading wine yeast transformants on the efficiency of wine processing and wine flavour

Louw, C., La Grange, D., Pretorius, I. S. & van Rensburg, P., 1 Oct 2006, In : Journal of Biotechnology. 125, 4, p. 447-461 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Flavors
Polysaccharides
Yeast
Yeasts

The grapevine polygalacturonase-inhibiting protein (VvPGIP1) reduces Botrytis cinerea susceptibility in transgenic tobacco and differentially inhibits fungal polygalacturonases

Joubert, D. A., Slaughter, A. R., Kemp, G., Becker, J. V. W., Krooshof, G. H., Bergmann, C., Benen, J., Pretorius, I. S. & Vivier, M. A., Dec 2006, In : Transgenic Research. 15, 6, p. 687-702 16 p.

Research output: Contribution to journalArticleResearchpeer-review

Botrytis
Polygalacturonase
polygalacturonase
Botrytis cinerea
Tobacco

The regulation of Saccharomyces cerevisiae FLO gene expression and Ca2+-dependent flocculation by Flo8p and Mss11p

Bester, M. C., Pretorius, I. S. & Bauer, F. F., Jun 2006, In : Current Genetics. 49, 6, p. 375-383 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Flocculation
Saccharomyces cerevisiae
Phenotype
Gene Expression
Cell Adhesion