Sakkie Pretorius

Professor

  • 12941 Citations
  • 56 h-Index
1983 …2020

Research output per year

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Research Outputs

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Article
2020

Bio-informational futures: the convergence of artificial intelligence and synthetic biology

Dixon, T. A., Curach, N. & Pretorius, I. S., 4 Mar 2020, In : EMBO Reports. 21, 3, p. 1-5 5 p., e50036.

Research output: Contribution to journalArticle

Blending wine yeast phenotypes with the aid of CRISPR DNA editing technologies

van Wyk, N., Kroukamp, H., Espinosa, M. I., von Wallbrunn, C., Wendland, J. & Pretorius, I. S., 2 Jul 2020, In : International Journal of Food Microbiology. 324, 7 p., 108615.

Research output: Contribution to journalArticle

2019
Open Access
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18 Downloads (Pure)

Building a global alliance of biofoundries

Hillson, N. J., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 others, Menolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., 9 May 2019, In : Nature Communications. 10, 1, p. 1-4 4 p., 2040.

Research output: Contribution to journalArticle

Open Access
File
20 Citations (Scopus)
38 Downloads (Pure)
Open Access
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2 Citations (Scopus)
10 Downloads (Pure)

Saccharomyces cerevisiae

Belda, I., Ruiz, J., Santos, A., Van Wyk, N. & Pretorius, I. S., Dec 2019, In : Trends in Genetics. 35, 12, p. 956-957 2 p.

Research output: Contribution to journalArticle

1 Citation (Scopus)
2018

Population sequencing reveals clonal diversity and ancestral inbreeding in the grapevine cultivar Chardonnay

Roach, M. J., Johnson, D. L., Bohlmann, J., van Vuuren, H. J. J., Jones, S. J. M., Pretorius, I. S., Schmidt, S. A. & Borneman, A. R., 20 Nov 2018, In : PLoS Genetics. 14, 11, p. 1-24 24 p., e1007807.

Research output: Contribution to journalArticle

Open Access
File
13 Citations (Scopus)
10 Downloads (Pure)

Systems-based approaches enable identification of gene targets which improve the flavour profile of low-ethanol wine yeast strains

Varela, C., Schmidt, S. A., Borneman, A. R., Pang, C. N. I., Krömerx, J. O., Khan, A., Song, X., Hodson, M. P., Solomon, M., Mayr, C. M., Hines, W., Pretorius, I. S., Baker, M. S., Roessner, U., Mercurio, M., Henschke, P. A., Wilkins, M. R. & Chambers, P. J., 1 Sep 2018, In : Metabolic Engineering. 49, p. 178-191 14 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)

Yeast 2.0—connecting the dots in the construction of the world's first functional synthetic eukaryotic genome

Pretorius, I. S. & Boeke, J. D., 1 Jun 2018, In : FEMS Yeast Research. 18, 4, p. 1-15 15 p., foy032.

Research output: Contribution to journalArticle

Open Access
File
28 Citations (Scopus)
40 Downloads (Pure)
2017
Open Access
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33 Downloads (Pure)
2016

Heterologous production of raspberry ketone in the wine yeast Saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion

Lee, D., Lloyd, N. D. R., Pretorius, I. S. & Borneman, A. R., 4 Mar 2016, In : Microbial Cell Factories. 15, p. 1-7 7 p., 49.

Research output: Contribution to journalArticle

Open Access
File
35 Citations (Scopus)
17 Downloads (Pure)
2015

Genomic insights into the Saccharomyces sensu stricto complex

Borneman, A. R. & Pretorius, I. S., 1 Feb 2015, In : Genetics. 199, 2, p. 281-291 11 p.

Research output: Contribution to journalArticle

48 Citations (Scopus)
2013

Novel wine yeast with mutations in YAP1 that produce less acetic acid during fermentation

Cordente, A. G., Cordero-Bueso, G., Pretorius, I. S. & Curtin, C. D., Jan 2013, In : FEMS Yeast Research. 13, 1, p. 62-73 12 p.

Research output: Contribution to journalArticle

24 Citations (Scopus)
2012

A breeding strategy to harness flavor diversity of Saccharomyces interspecific hybrids and minimize hydrogen sulfide production

Bizaj, E., Cordente, A. G., Bellon, J. R., Raspor, P., Curtin, C. D. & Pretorius, I. S., Jun 2012, In : FEMS Yeast Research. 12, 4, p. 456-465 10 p.

Research output: Contribution to journalArticle

33 Citations (Scopus)

De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast dekkera bruxellensis AWRI1499

Curtin, C. D., Borneman, A. R., Chambers, P. J. & Pretorius, I. S., 28 Mar 2012, In : PLoS ONE. 7, 3, p. 1-10 10 p., e33840.

Research output: Contribution to journalArticle

Open Access
File
81 Citations (Scopus)
15 Downloads (Pure)

Effect of ammonium nitrogen supplementation of grape juice on wine volatiles and non-volatiles composition of the aromatic grape variety Albarino

Vilanova, M., Siebert, T. E., Varela, C., Pretorius, I. S. & Henschke, P. A., 1 Jul 2012, In : Food Chemistry. 133, 1, p. 124-131 8 p.

Research output: Contribution to journalArticle

33 Citations (Scopus)

Evaluation of gene modification strategies for the development of low-alcohol-wine Yeasts

Varela, C., Kutyna, D. R., Solomon, M. R., Black, C. A., Borneman, A., Henschke, P. A., Pretorius, I. S. & Chambers, P. J., Sep 2012, In : Applied and Environmental Microbiology. 78, 17, p. 6068-6077 10 p.

Research output: Contribution to journalArticle

60 Citations (Scopus)

The genome sequence of the wine yeast VIN7 reveals an allotriploid hybrid genome with Saccharomyces cerevisiae and Saccharomyces kudriavzevii origins

Borneman, A. R., Desany, B. A., Riches, D., Affourtit, J. P., Forgan, A. H., Pretorius, I. S., Egholm, M. & Chambers, P. J., Feb 2012, In : FEMS Yeast Research. 12, 1, p. 88-96 9 p.

Research output: Contribution to journalArticle

78 Citations (Scopus)
2011

Whole-genome comparison reveals novel genetic elements that characterize the genome of industrial strains of saccharomyces cerevisiae

Borneman, A. R., Desany, B. A., Riches, D., Affourtit, J. P., Forgan, A. H., Pretorius, I. S., Egholm, M. & Chambers, P. J., 7 Feb 2011, In : PLoS Genetics. 7, 2, p. 1-10 10 p., e1001287.

Research output: Contribution to journalArticle

Open Access
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183 Citations (Scopus)
9 Downloads (Pure)
2010

Preliminary evaluation of infrared spectroscopy for the differentiation of Brettanomyces bruxellensis strains isolated from red wines

Oelofse, A., Malherbe, S., Pretorius, I. S. & Du Toit, M., 15 Oct 2010, In : International Journal of Food Microbiology. 143, 3, p. 136-142 7 p.

Research output: Contribution to journalArticle

11 Citations (Scopus)

The effect of multiple yeasts co-inoculations on Sauvignon Blanc wine aroma composition, sensory properties and consumer preference

King, E. S., Kievit, R. L., Curtin, C., Swiegers, J. H., Pretorius, I. S., Bastian, S. E. P. & Leigh Francis, I., 1 Oct 2010, In : Food Chemistry. 122, 3, p. 618-626 9 p.

Research output: Contribution to journalArticle

51 Citations (Scopus)
2009

Differential synthesis of fermentative aroma compounds of two related commercial wine yeast strains

Molina, A. M., Guadalupe, V., Varela, C., Swiegers, J. H., Pretorius, I. S. & Agosin, E., 15 Nov 2009, In : Food Chemistry. 117, 2, p. 189-195 7 p.

Research output: Contribution to journalArticle

61 Citations (Scopus)

Establishing a risk-assessment process for release of genetically modified wine yeast into the environment

Schoeman, H., Wolfaardt, G. M., Botha, A., Van Rensburg, P. & Pretorius, I. S., Aug 2009, In : Canadian Journal of Microbiology. 55, 8, p. 990-1002 13 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Identification of yeast population dynamics of spontaneous fermentation in Beijing wine region, China

Sun, H., Ma, H., Hao, M., Pretorius, I. S. & Chen, S., 2009, In : Annals of Microbiology. 59, 1, p. 69-76 8 p.

Research output: Contribution to journalArticle

29 Citations (Scopus)

Isolation of sulfite reductase variants of a commercial wine yeast with significantly reduced hydrogen sulfide production

Cordente, A. G., Heinrich, A., Pretorius, I. S. & Swiegers, J. H., May 2009, In : FEMS Yeast Research. 9, 3, p. 446-459 14 p.

Research output: Contribution to journalArticle

57 Citations (Scopus)

The influence of yeast on the aroma of Sauvignon Blanc wine

Swiegers, J. H., Kievit, R. L., Siebert, T., Lattey, K. A., Bramley, B. R., Francis, I. L., King, E. S. & Pretorius, I. S., Apr 2009, In : Food Microbiology. 26, 2, p. 204-211 8 p.

Research output: Contribution to journalArticle

99 Citations (Scopus)
2008

Coinoculated fermentations using Saccharomyces yeasts affect the volatile composition and sensory properties of Vitis vinifera L. cv. Sauvignon Blanc wines

King, E. S., Swiegers, J. H., Travis, B., Francis, I. L., Bastian, S. E. P. & Pretorius, I. S., 26 Nov 2008, In : Journal of Agricultural and Food Chemistry. 56, 22, p. 10829-10837 9 p.

Research output: Contribution to journalArticle

53 Citations (Scopus)

Comparative genome analysis of a Saccharomyces cerevisiae wine strain

Borneman, A. R., Forgan, A. H., Pretorius, I. S. & Chambers, P. J., Nov 2008, In : FEMS Yeast Research. 8, 7, p. 1185-1195 11 p.

Research output: Contribution to journalArticle

114 Citations (Scopus)

Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae

Govender, P., Domingo, J. L., Bester, M. C., Pretorius, I. S. & Bauer, F. F., Oct 2008, In : Applied and Environmental Microbiology. 74, 19, p. 6041-6052 12 p.

Research output: Contribution to journalArticle

90 Citations (Scopus)

Correlation between glucose/fructose discrepancy and hexokinase kinetic properties in different Saccharomyces cerevisiae wine yeast strains

Berthels, N. J., Cordero Otero, R. R., Bauer, F. F., Pretorius, I. S. & Thevelein, J. M., Jan 2008, In : Applied Microbiology and Biotechnology. 77, 5, p. 1083-1091 9 p.

Research output: Contribution to journalArticle

43 Citations (Scopus)

Significance of Brettanomyces and Dekkera during Winemaking: A Synoptic Review

Oelofse, A., Pretorius, I. S. & du Toit, M., 2008, In : South African Journal of Enology and Viticulture. 29, 2, p. 128-144 17 p.

Research output: Contribution to journalArticle

97 Citations (Scopus)

The effect of flocculation on the efficiency of raw-starch fermentation by Saccharomyces cerevisiae producing the Lipomyces kononenkoae LKA1-encoded α-amylase

Ramachandran, N., Joubert, L., Gundlapalli, S. B., Cordero Otero, R. R. & Pretorius, I. S., 2008, In : Annals of Microbiology. 58, 1, p. 99-108 10 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)

The Thr505 and Ser557 residues of the AGT1-encoded α-glucoside transporter are critical for maltotriose transport in Saccharomyces cerevisiae

Smit, A., Moses, S. G., Pretorius, I. S. & Cordero Otero, R. R., Apr 2008, In : Journal of Applied Microbiology. 104, 4, p. 1103-1111 9 p.

Research output: Contribution to journalArticle

9 Citations (Scopus)
2007

A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation

Thanvanthri Gururajan, V., Gorwa-Grauslund, M. F., Hahn-Hägerdal, B., Pretorius, I. S. & Cordero Otero, R. R., 2007, In : Annals of Microbiology. 57, 1, p. 85-92 8 p.

Research output: Contribution to journalArticle

10 Citations (Scopus)

Assimilable nitrogen utilisation and production of volatile and non-volatile compounds in chemically defined medium by Saccharomyces cerevisiae wine yeasts

Vilanova, M., Ugliano, M., Varela, C., Siebert, T., Pretorius, I. S. & Henschke, P. A., Nov 2007, In : Applied Microbiology and Biotechnology. 77, 1, p. 145-157 13 p.

Research output: Contribution to journalArticle

147 Citations (Scopus)

Development and characterisation of a recombinant Saccharomyces cerevisiae mutant strain with enhanced xylose fermentation properties

Thanvanthri Gururajan, V., Van Rensburg, P., Hahn-Hägerdal, B., Pretorius, I. S. & Cordero Otero, R. R., 2007, In : Annals of Microbiology. 57, 4, p. 599-607 9 p.

Research output: Contribution to journalArticle

4 Citations (Scopus)

Differences among AGT1-encoded α-glucoside transporters and their ability to transport maltotriose in Saccharomyces yeasts

Smit, A., Cordero Otero, R. R. & Pretorius, I. S., 2007, In : Annals of Microbiology. 57, 1, p. 77-84 8 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)

Effect of the cellulose-binding domain on the catalytic activity of a β-glucosidase from Saccharomycopsis fibuligera

Gundllapalli, S. B., Pretorius, I. S. & Cordero Otero, R. R., Jun 2007, In : Journal of Industrial Microbiology and Biotechnology. 34, 6, p. 413-421 9 p.

Research output: Contribution to journalArticle

16 Citations (Scopus)

Engineering volatile thiol release in Saccharomyces cerevisiae for improved wine aroma

Swiegers, J. H., Capone, D. L., Pardon, K. H., Elsey, G. M., Sefton, M. A., Francis, I. L. & Pretorius, I. S., Jul 2007, In : Yeast. 24, 7, p. 561-574 14 p.

Research output: Contribution to journalArticle

104 Citations (Scopus)

Influence of wine fermentation temperature on the synthesis of yeast-derived volatile aroma compounds

Molina, A. M., Swiegers, J. H., Varela, C., Pretorius, I. S. & Agosin, E., Nov 2007, In : Applied Microbiology and Biotechnology. 77, 3, p. 675-687 13 p.

Research output: Contribution to journalArticle

143 Citations (Scopus)

Modulating aroma compounds during wine fermentation by manipulating carnitine acetyltransferases in Saccharomyces cerevisiae

Cordente, A. G., Swiegers, J. H., Hegardt, F. G. & Pretorius, I. S., Feb 2007, In : FEMS Microbiology Letters. 267, 2, p. 159-166 8 p.

Research output: Contribution to journalArticle

17 Citations (Scopus)

Molecular cloning and functional expression of a novel Neurospora crassa xylose reductase in Saccharomyces cerevisiae in the development of a xylose fermenting strain

Thanvanthri Gururajan, V., Pretorius, I. S. & Cordero Otero, R. R., 2007, In : Annals of Microbiology. 57, 2, p. 223-231 9 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)

The heterologous expression of polysaccharidase-encoding genes with oenological relevance in Saccharomyces cerevisiae

Van Rensburg, P., Strauss, M. L. A., Lambrechts, M. G., Cordero Otero, R. R. & Pretorius, I. S., Dec 2007, In : Journal of Applied Microbiology. 103, 6, p. 2248-2257 10 p.

Research output: Contribution to journalArticle

18 Citations (Scopus)
2006
5 Citations (Scopus)

Modulation of volatile thiol and ester aromas by modified wine yeast

Swiegers, J. H., Willmott, R., Hill-Ling, A., Capone, D. L., Pardon, K. H., Elsey, G. M., Howell, K. S., de Barros Lopes, M. A., Sefton, M. A., Lilly, M. & Pretorius, I. S., 2006, In : Developments in Food Science. 43, C, p. 113-116 4 p.

Research output: Contribution to journalArticle

38 Citations (Scopus)

Rapid screening of the fermentation profiles of wine yeasts by Fourier transform infrared spectroscopy

Nieuwoudt, H. H., Pretorius, I. S., Bauer, F. F., Nel, D. G. & Prior, B. A., Nov 2006, In : Journal of Microbiological Methods. 67, 2, p. 248-256 9 p.

Research output: Contribution to journalArticle

35 Citations (Scopus)

Regulation of respiratory growth by Ras: The glyoxylate cycle mutant, cit2Δ, is suppressed by RAS2

Swiegers, J. H., Pretorius, I. S. & Bauer, F. F., Sep 2006, In : Current Genetics. 50, 3, p. 161-171 11 p.

Research output: Contribution to journalArticle

9 Citations (Scopus)

The effect of increased branched-chain amino acid transaminase activity in yeast on the production of higher alcohols and on the flavour profiles of wine and distillates

Lilly, M., Bauer, F. F., Styger, G., Lambrechts, M. G. & Pretorius, I. S., Aug 2006, In : FEMS Yeast Research. 6, 5, p. 726-743 18 p.

Research output: Contribution to journalArticle

103 Citations (Scopus)

The effect of increased yeast alchohol acetyltransferase and esterase activity on the flavour profiles of wine and distillates

Lilly, M., Bauer, F. F., Lambrechts, M. G., Swiegers, J. H., Cozzolino, D. & Pretorius, I. S., 15 Jul 2006, In : Yeast. 23, 9, p. 641-659 19 p.

Research output: Contribution to journalArticle

136 Citations (Scopus)

The effect of polysaccharide-degrading wine yeast transformants on the efficiency of wine processing and wine flavour

Louw, C., La Grange, D., Pretorius, I. S. & van Rensburg, P., 1 Oct 2006, In : Journal of Biotechnology. 125, 4, p. 447-461 15 p.

Research output: Contribution to journalArticle

27 Citations (Scopus)