Sakkie Pretorius

Professor

  • 12288 Citations
  • 55 h-Index
1983 …2020
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Research Outputs 1983 2020

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Article

A breeding strategy to harness flavor diversity of Saccharomyces interspecific hybrids and minimize hydrogen sulfide production

Bizaj, E., Cordente, A. G., Bellon, J. R., Raspor, P., Curtin, C. D. & Pretorius, I. S., Jun 2012, In : FEMS Yeast Research. 12, 4, p. 456-465 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Hydrogen Sulfide
Saccharomyces
Wine
Breeding
Yeasts

A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation

Thanvanthri Gururajan, V., Gorwa-Grauslund, M. F., Hahn-Hägerdal, B., Pretorius, I. S. & Cordero Otero, R. R., 2007, In : Annals of Microbiology. 57, 1, p. 85-92 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Catabolite Repression
Xylose
Fermentation
Saccharomyces cerevisiae
Glucose

A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome

Wingfield, B. D., Southgate, V. J., Pretorius, I. S. & Van Vuuren, H. J. J., 1990, In : Yeast. 6, 2, p. 159-169 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Double-Stranded RNA
RNA
Yeast
Saccharomyces cerevisiae
Genes

A multicopy suppressor gene, MSS10, restores STA2 expression in Saccharomyces cerevisiae strains containing the STA10 represser gene

Lambrechts, M. G., Sollitti, P., Murmur, J. & Pretorius, I. S., 1996, In : Current Genetics. 29, 6, p. 523-529 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Suppressor Genes
Saccharomyces cerevisiae
Glucan 1,4-alpha-Glucosidase
Genes
beta-Fructofuranosidase

Amylolytic enzymes from the yeast Lipomyces kononenkoae

Ramachandran, N., Pretorius, I. S. & Cordero Otero, R. R., 2005, In : Biologia - Section Cellular and Molecular Biology. 60, SUPPL. 16, p. 103-110 8 p.

Research output: Contribution to journalArticleResearch

Lipomyces
starch
Starch
Yeast
yeast
Pectobacterium carotovorum
Polygalacturonase
Saccharomyces cerevisiae
Yeasts
Mating Factor

Assimilable nitrogen utilisation and production of volatile and non-volatile compounds in chemically defined medium by Saccharomyces cerevisiae wine yeasts

Vilanova, M., Ugliano, M., Varela, C., Siebert, T., Pretorius, I. S. & Henschke, P. A., Nov 2007, In : Applied Microbiology and Biotechnology. 77, 1, p. 145-157 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Yeast
Saccharomyces cerevisiae
Nitrogen
Yeasts
Open Access
File
Benchmarking
Metabolism
Yeast
Methanol
Saccharomyces cerevisiae

Building a global alliance of biofoundries

Hillson, N. J., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., Bell, D. J., Le Feuvre, R., Friedman, D. C., Fu, X., Gold, N. D., Herrgård, M. J., Holowko, M. B., Johnson, J. R., Johnson, R. A., Keasling, J. D., Kitney, R. I., Kondo, A., Liu, C., Martin, V. J. J. & 16 othersMenolascina, F., Ogino, C., Patron, N. J., Pavan, M., Poh, C. L., Pretorius, I. S., Rosser, S. J., Scrutton, N. S., Storch, M., Tekotte, H., Travnik, E., Vickers, C. E., Yew, W. S., Yuan, Y., Zhao, H. & Freemont, P. S., 9 May 2019, In : Nature Communications. 10, 10, p. 1-4 4 p., 2040.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
biotechnology
Biotechnology
organisms
Testing
Research

Carnitine biosynthesis in Neurospora crassa: Identification of a cDNA coding for ε-N-trimethyllysine hydroxylase and its functional expression in Saccharomyces cerevisiae

Swiegers, J. H., Vaz, F. M., Pretorius, I. S., Wanders, R. J. A. & Bauer, F. F., 23 Apr 2002, In : FEMS Microbiology Letters. 210, 1, p. 19-23 5 p.

Research output: Contribution to journalArticleResearchpeer-review

epsilon-N-trimethyllysine hydroxylase
Neurospora crassa
Carnitine
Saccharomyces cerevisiae
Complementary DNA

Carnitine-dependent metabolic activities in Saccharomyces cerevisiae: Three carnitine acetyltransferases are essential in a carnitine-dependent strain

Swiegers, J. H., Dippenaar, N., Pretorius, I. S. & Bauer, F. F., 2001, In : Yeast. 18, 7, p. 585-595 11 p.

Research output: Contribution to journalArticleResearchpeer-review

carnitine acetyltransferase
Carnitine O-Acetyltransferase
Carnitine
carnitine
Yeast

Cellular Differentiation in Response to Nutrient Availability: The Repressor of Meiosis, Rme1p, Positively Regulates Invasive Growth in Saccharomyces cerevisiae

Van Dyk, D., Hansson, G., Pretorius, I. S. & Bauer, F. F., Nov 2003, In : Genetics. 165, 3, p. 1045-1058 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Meiosis
Saccharomyces cerevisiae
Food
Growth
Haploidy

Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae

Bayly, J. C., Douglas, L. M., Pretorius, I. S., Bauer, F. F. & Dranginis, A. M., Dec 2005, In : FEMS Yeast Research. 5, 12, p. 1151-1156 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Flocculation
Saccharomyces cerevisiae
Maltose
Mannose
Sucrose

Characterization of a novel α-amylase from Lipomyces kononenkoae and expression of its gene (LKA1) in Saccharomyces cerevisiae

Steyn, A. J. C. & Pretorius, I. S., Nov 1995, In : Current Genetics. 28, 6, p. 526-533 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
alpha-Amylases
Glycosylation
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae

Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts

Cordero Otero, R. R., Ubeda Iranzo, J. F., Briones-Perez, A. I., Potgieter, N., Villena, M. A., Pretorius, I. S. & Van Rensburg, P., Sep 2003, In : Journal of Food Science. 68, 8, p. 2564-2569 6 p.

Research output: Contribution to journalArticleResearchpeer-review

beta-Glucosidase
Wine
beta-glucosidase
wines
Yeasts

Cloning, mapping and characterization of a genomic copy of the Lipomyces kononenkoae α-amylase-encoding gene (LKA1)

Steyn, A. J. C., Marmur, J. & Pretorius, I. S., Aug 1996, In : Yeast. 12, 10, p. 925-937 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Amylases
Gene encoding
alpha-Amylases
Cloning

Cloning, sequence analysis and expression in yeasts of a cDNA containing a Lipomyces kononenkoae α-amylase-encoding gene

Steyn, A. J. C., Marmur, J. & Pretorius, I. S., 1 Dec 1995, In : Gene. 166, 1, p. 65-71 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
alpha-Amylases
Sequence Analysis
Organism Cloning
Complementary DNA

Cloning and expression of an Aspergillus kawachii endo-1,4-β-xylanase gene in Saccharomyces cerevisiae

Crous, J. M., Pretorius, I. S. & van Zyl, W. H., Oct 1995, In : Current Genetics. 28, 5, p. 467-473 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Endo-1,4-beta Xylanases
Aspergillus
Saccharomyces cerevisiae
Organism Cloning
Genes

Cloning and expression of the Clostridium thermosulfurogenes d-xylose isomerase gene (xylA) in Saccharomyces cerevlsiae

Moes, C. J., Pretorius, I. S. & Van Zyl, W. H., 1996, In : Biotechnology Letters. 18, 3, p. 269-274 6 p.

Research output: Contribution to journalArticleResearchpeer-review

xylose isomerase
Clostridium
Saccharomyces
Xylose
Cloning

Cloning and expression of the α-L-arabinofuranosidase gene (ABF2) of Aspergillus niger in Saccharomyces cerevisiae

Crous, J. M., Pretorius, I. S. & Van Zyl, W. H., 1996, In : Applied Microbiology and Biotechnology. 46, 3, p. 256-260 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Aspergillus niger
Cloning
Aspergillus
Yeast
Saccharomyces cerevisiae

Cloning of the Bacillus pumilus β-xylosidase gene (xynB) and its expression in Saccharomyces cerevisiae

La Grange, D. C., Pretorius, I. S. & Van Zyl, W. H., 1997, In : Applied Microbiology and Biotechnology. 47, 3, p. 262-266 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Cloning
Bacilli
Yeast
Saccharomyces cerevisiae
Organism Cloning

Cloning of two β-xylanase-encoding genes from Aspergillus niger and their expression in Saccharomyces cerevisiae

Luttig, M., Pretorius, I. S. & Van Zyl, W. H., 1997, In : Biotechnology Letters. 19, 5, p. 411-415 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Endo-1,4-beta Xylanases
Gene encoding
Aspergillus niger
Cloning
Aspergillus
Pectobacterium chrysanthemi
Polygalacturonase
Gene encoding
Yeast
Saccharomyces cerevisiae

Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-β-1,4-glucanase gene in Saccharomyces cerevisiae

Van Rensburg, P., Van Zyl, W. H. & Pretorius, I. S., Aug 1996, In : Current Genetics. 30, 3, p. 246-250 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Cellulose 1,4-beta-Cellobiosidase
Phanerochaete
Cellulase
Saccharomyces cerevisiae
Genes

Co-expression of a Saccharomyces diastaticus glucoamylase-encoding gene and a Bacillus amyloliquefaciens α-amylase-encoding gene in Saccharomyces cerevisiae

Steyn, A. J. C. & Pretorius, I. S., 1991, In : Gene. 100, C, p. 85-93 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Saccharomyces
alpha-Amylases
Saccharomyces cerevisiae
Plasmids

Coexpression of the Bacillus pumilus β-xylosidase (xynB) gene with the Trichoderma reesei β-xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae

La Grange, D. C., Claeyssens, M., Pretorius, I. S. & Van Zyl, W. H., 2000, In : Applied Microbiology and Biotechnology. 54, 2, p. 195-200 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Endo-1,4-beta Xylanases
Trichoderma
Bacilli
Yeast
Saccharomyces cerevisiae

Coinoculated fermentations using Saccharomyces yeasts affect the volatile composition and sensory properties of Vitis vinifera L. cv. Sauvignon Blanc wines

King, E. S., Swiegers, J. H., Travis, B., Francis, I. L., Bastian, S. E. P. & Pretorius, I. S., 26 Nov 2008, In : Journal of Agricultural and Food Chemistry. 56, 22, p. 10829-10837 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
Wine
Vitis
Vitis vinifera
Yeast

Comparative genome analysis of a Saccharomyces cerevisiae wine strain

Borneman, A. R., Forgan, A. H., Pretorius, I. S. & Chambers, P. J., Nov 2008, In : FEMS Yeast Research. 8, 7, p. 1185-1195 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Saccharomyces cerevisiae
Genome
Single Nucleotide Polymorphism
Ethanol

Comparison of commercial enzymes for the processing of marula pulp, wine, and spirits

Fundira, M., Blom, M., Pretorius, I. S. & Van Rensburg, P., Aug 2002, In : Journal of Food Science. 67, 6, p. 2346-2351 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Anacardiaceae
Wine
pulp
wines
juices

Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae

Govender, P., Domingo, J. L., Bester, M. C., Pretorius, I. S. & Bauer, F. F., Oct 2008, In : Applied and Environmental Microbiology. 74, 19, p. 6041-6052 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Flocculation
Dominant Genes
flocculation
Fermentation
fermentation

Correlation between glucose/fructose discrepancy and hexokinase kinetic properties in different Saccharomyces cerevisiae wine yeast strains

Berthels, N. J., Cordero Otero, R. R., Bauer, F. F., Pretorius, I. S. & Thevelein, J. M., Jan 2008, In : Applied Microbiology and Biotechnology. 77, 5, p. 1083-1091 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Fructose
Hexokinase
Wine
Yeast
Glucose

Degradation of Xylan to D-Xylose by Recombinant Saccharomyces cerevisiae Coexpressing the Aspergillus niger β-Xylosidase (xlnD) and the Trichoderma reesei Xylanase II (xyn2) Genes

La Grange, D. C., Pretorius, I. S., Claeyssens, M. & Van Zyl, W. H., Dec 2001, In : Applied and Environmental Microbiology. 67, 12, p. 5512-5519 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Endo-1,4-beta Xylanases
xylan 1,4-beta-xylosidase
Xylans
Trichoderma reesei
Trichoderma

De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast dekkera bruxellensis AWRI1499

Curtin, C. D., Borneman, A. R., Chambers, P. J. & Pretorius, I. S., 28 Mar 2012, In : PLoS ONE. 7, 3, p. 1-10 10 p., e33840.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
File
Dekkera
Spoilage
Triploidy
Wine
triploidy

Development and assessment of a recombinant Saccharomyces cerevisiae wine yeast producing two aroma-enhancing β-glucosidases encoded by the Saccharomycopsis fibuligera BGL1 and BGL2 genes

Van Rensburg, P., Stidwell, T., Lambrechts, M. G., Cordero Otero, R. & Pretorius, I. S., 2005, In : Annals of Microbiology. 55, 1, p. 33-42 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomycopsis
Cellulases
Wine
Saccharomyces cerevisiae
Yeasts

Development and characterisation of a recombinant Saccharomyces cerevisiae mutant strain with enhanced xylose fermentation properties

Thanvanthri Gururajan, V., Van Rensburg, P., Hahn-Hägerdal, B., Pretorius, I. S. & Cordero Otero, R. R., 2007, In : Annals of Microbiology. 57, 4, p. 599-607 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Xylose
Fermentation
Saccharomyces cerevisiae
Genes
D-Xylulose Reductase

Development of a polysaccharide degrading strain of Saccharomyces cerevisiae

Petersen, S. H., Van Zyl, W. H. & Pretorius, I. S., 1998, In : Biotechnology Techniques. 12, 8, p. 615-619 5 p.

Research output: Contribution to journalArticleResearchpeer-review

beta-Glucosidase
Yeast
Biomass
Polysaccharides
Saccharomyces cerevisiae

Development of a screening method for the identification of a novel Saccharomyces cerevisiae mutant over-expressing Trichoderma reesei cellobiohydrolase II

Gundllapalli, S. B., Otero, R. R. C. & Pretorius, I. S., 2006, In : Annals of Microbiology. 56, 2, p. 143-150 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Cellulose 1,4-beta-Cellobiosidase
Trichoderma
Saccharomyces cerevisiae
Enzymes
Cellulose

Differences among AGT1-encoded α-glucoside transporters and their ability to transport maltotriose in Saccharomyces yeasts

Smit, A., Cordero Otero, R. R. & Pretorius, I. S., 2007, In : Annals of Microbiology. 57, 1, p. 77-84 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
Glucosides
Yeasts
Maltose
Membrane Transport Proteins

Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae α-amylase in industrial strains of Saccharomyces cerevisiae

Gundllapalli Moses, S. B., Cordero Otero, R. R., La Grange, D. C., Van Rensburg, P. & Pretorius, I. S., 2002, In : Biotechnology Letters. 24, 8, p. 651-656 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Amylases
alpha-Amylases
Starch
Yeast

Differential synthesis of fermentative aroma compounds of two related commercial wine yeast strains

Molina, A. M., Guadalupe, V., Varela, C., Swiegers, J. H., Pretorius, I. S. & Agosin, E., 15 Nov 2009, In : Food Chemistry. 117, 2, p. 189-195 7 p.

Research output: Contribution to journalArticleResearchpeer-review

wine yeasts
Wine
Flavors
odor compounds
Yeast

Discrepancy in glucose and fructose utilisation during fermentation by Saccharomyces cerevisiae wine yeast strains

Berthels, N. J., Cordero Otero, R. R., Bauer, F. F., Thevelein, J. M. & Pretorius, I. S., May 2004, In : FEMS Yeast Research. 4, 7, p. 683-689 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Fructose
Fermentation
Saccharomyces cerevisiae
Yeasts

Divergent regulation of the evolutionarily closely related promoters of the Saccharomyces cerevisiae STA2 and MUC1 genes

Gagiano, M., Van Dyk, D., Bauer, F. F., Lambrechts, M. G. & Pretorius, I. S., Oct 1999, In : Journal of Bacteriology. 181, 20, p. 6497-6508 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces cerevisiae
Genes
Flocculation
Catabolite Repression
Glucan 1,4-alpha-Glucosidase

Domain engineering of Saccharomyces cerevisiae exoglucanases

Gundllapalli Moses, S. B., Cordero Otero, R. R. & Pretorius, I. S., Mar 2005, In : Biotechnology Letters. 27, 5, p. 355-362 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Cellulose
Yeast
Saccharomyces cerevisiae
Enzymes
Cellulose 1,4-beta-Cellobiosidase

Effect of ammonium nitrogen supplementation of grape juice on wine volatiles and non-volatiles composition of the aromatic grape variety Albarino

Vilanova, M., Siebert, T. E., Varela, C., Pretorius, I. S. & Henschke, P. A., 1 Jul 2012, In : Food Chemistry. 133, 1, p. 124-131 8 p.

Research output: Contribution to journalArticleResearchpeer-review

grape juice
ammonium nitrogen
Wine
Vitis
Ammonium Compounds

Effect of increased yeast alcohol acetyltransferase activity on flavor profiles of wine and distillates

Lilly, M., Lambrechts, M. G. & Pretorius, I. S., Feb 2000, In : Applied and Environmental Microbiology. 66, 2, p. 744-753 10 p.

Research output: Contribution to journalArticleResearchpeer-review

distillates
acetyltransferases
Acetyltransferases
Wine
yeast

Effect of the cellulose-binding domain on the catalytic activity of a β-glucosidase from Saccharomycopsis fibuligera

Gundllapalli, S. B., Pretorius, I. S. & Cordero Otero, R. R., Jun 2007, In : Journal of Industrial Microbiology and Biotechnology. 34, 6, p. 413-421 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomycopsis
beta-Glucosidase
Cellulose
Catalyst activity
Catalytic Domain

Electron microscopy of the K2 killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast

Vadasz, A. S., Jagganath, D. B., Pretorius, I. S. & Gupthar, A. S., 2000, In : Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 78, 2, p. 117-122 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Saccharomyces cerevisiae
Electron Microscopy
Yeasts
Methylene Blue

Engineering volatile thiol release in Saccharomyces cerevisiae for improved wine aroma

Swiegers, J. H., Capone, D. L., Pardon, K. H., Elsey, G. M., Sefton, M. A., Francis, I. L. & Pretorius, I. S., Jul 2007, In : Yeast. 24, 7, p. 561-574 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
thiols
Sulfhydryl Compounds
Yeast
Saccharomyces cerevisiae

Engineering yeast for efficient cellulose degradation

Van Rensburg, P., Van Zyl, W. H. & Pretorius, I. S., 15 Jan 1998, In : Yeast. 14, 1, p. 67-76 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Cellulase
Cellulose
Yeast
Yeasts
Saccharomyces cerevisiae

Enhancing volatile phenol concentrations in wine by expressing various phenolic acid decarboxylase genes in Saccharomyces cerevisiae

Smit, A., Cordero Otero, R. R., Lambrechts, M. G., Pretorius, I. S. & Van Rensburg, P., 13 Aug 2003, In : Journal of Agricultural and Food Chemistry. 51, 17, p. 4909-4915 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Phenol
phenolic acids
Yeast
phenol