Sakkie Pretorius

Professor

  • 12214 Citations
  • 54 h-Index
1983 …2020
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Research Outputs 1983 2019

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Article
2005

Amylolytic enzymes from the yeast Lipomyces kononenkoae

Ramachandran, N., Pretorius, I. S. & Cordero Otero, R. R., 2005, In : Biologia - Section Cellular and Molecular Biology. 60, SUPPL. 16, p. 103-110 8 p.

Research output: Contribution to journalArticleResearch

Lipomyces
starch
Starch
Yeast
yeast

Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae

Bayly, J. C., Douglas, L. M., Pretorius, I. S., Bauer, F. F. & Dranginis, A. M., Dec 2005, In : FEMS Yeast Research. 5, 12, p. 1151-1156 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Flocculation
Saccharomyces cerevisiae
Maltose
Mannose
Sucrose

Development and assessment of a recombinant Saccharomyces cerevisiae wine yeast producing two aroma-enhancing β-glucosidases encoded by the Saccharomycopsis fibuligera BGL1 and BGL2 genes

Van Rensburg, P., Stidwell, T., Lambrechts, M. G., Cordero Otero, R. & Pretorius, I. S., 2005, In : Annals of Microbiology. 55, 1, p. 33-42 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomycopsis
Cellulases
Wine
Saccharomyces cerevisiae
Yeasts

Domain engineering of Saccharomyces cerevisiae exoglucanases

Gundllapalli Moses, S. B., Cordero Otero, R. R. & Pretorius, I. S., Mar 2005, In : Biotechnology Letters. 27, 5, p. 355-362 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Cellulose
Yeast
Saccharomyces cerevisiae
Enzymes
Cellulose 1,4-beta-Cellobiosidase

Genetic determinants of volatile-thiol release by Saccharomyces cerevisiae during wine fermentation

Howell, K. S., Klein, M., Swiegers, J. H., Hayasaka, Y., Elsey, G. M., Fleet, G. H., Høj, P. B., Pretorius, I. S. & De Barros Lopes, M. A., Sep 2005, In : Applied and Environmental Microbiology. 71, 9, p. 5420-5426 7 p.

Research output: Contribution to journalArticleResearchpeer-review

carbon-sulfur lyases
thiol
Wine
thiols
Sulfhydryl Compounds

Grape and wine biotechnology: Challenges, opportunities and potential benefits

Pretorius, I. S. & Høj, P. B., 2005, In : Australian Journal of Grape and Wine Research. 11, 2, p. 83-108 26 p.

Research output: Contribution to journalArticleResearchpeer-review

biotechnology
wines
grapes
arts
winemaking

Mss11p is a central element of the regulatory network that controls FLO11 expression and invasive growth in Saccharomyces cerevisiae

Van Dyk, D., Pretorius, I. S. & Bauer, F. F., Jan 2005, In : Genetics. 169, 1, p. 91-106 16 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces cerevisiae
Growth
Proteins
Cell Wall
Genes

Olfaction and taste: Human perception, physiology and genetics

Swiegers, J. H., Chambers, P. J. & Pretorius, I. S., 2005, In : Australian Journal of Grape and Wine Research. 11, 2, p. 109-113 5 p.

Research output: Contribution to journalArticleResearchpeer-review

smell
physiology
odor compounds
wines
olfactory receptors

The effect of sulphur dioxide and oxygen on the viability and culturability of a strain of Acetobacter pasteurianus and a strain of Brettanomyces bruxellensis isolated from wine

Du Toit, W. J., Pretorius, I. S. & Lonvaud-Funel, A., 2005, In : Journal of Applied Microbiology. 98, 4, p. 862-871 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Brettanomyces
Acetobacter pasteurianus
Acetobacter
Sulfur Dioxide
Wine

Yeast and bacterial modulation of wine aroma and flavour

Swiegers, J. H., Bartowsky, E. J., Henschke, P. A. & Pretorius, I. S., 2005, In : Australian Journal of Grape and Wine Research. 11, 2, p. 139-173 35 p.

Research output: Contribution to journalArticleResearchpeer-review

wines
flavor
odors
yeasts
grapes
2004

Discrepancy in glucose and fructose utilisation during fermentation by Saccharomyces cerevisiae wine yeast strains

Berthels, N. J., Cordero Otero, R. R., Bauer, F. F., Thevelein, J. M. & Pretorius, I. S., May 2004, In : FEMS Yeast Research. 4, 7, p. 683-689 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Fructose
Fermentation
Saccharomyces cerevisiae
Yeasts

Expression of 2 Lipomyces kononenkoae α-amylase genes in selected whisky yeast strains

La Grange-Nel, K., Smit, A., Cordero Otero, R. R., Lambrechts, M. G., Willemse, Q., Van Rensburg, P. & Pretorius, I. S., Sep 2004, In : Journal of Food Science. 69, 7, p. M175-M181 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
whisky
alpha-Amylases
alpha-amylase
Yeasts

Identification of lactic acid bacteria isolated from South African brandy base wines

Du Plessis, H. W., Dicks, L. M. T., Pretorius, I. S., Lambrechts, M. G. & Du Toit, M., 15 Feb 2004, In : International Journal of Food Microbiology. 91, 1, p. 19-29 11 p.

Research output: Contribution to journalArticleResearchpeer-review

brandy
Wine
lactic acid bacteria
wines
Lactic Acid

Principal component analysis applied to Fourier transform infrared spectroscopy for the design of calibration sets for glycerol prediction models in wine and for the detection and classification of outlier samples

Nieuwoudt, H. H., Prior, B. A., Pretorius, I. S., Manley, M. & Bauer, F. F., 16 Jun 2004, In : Journal of Agricultural and Food Chemistry. 52, 12, p. 3726-3735 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Fourier transform infrared spectroscopy
Fourier Transform Infrared Spectroscopy
Principal Component Analysis
Glycerol

Redesign of carnitine acetyltransferase specificity by protein engineering

Cordente, A. G., López-Viñas, E., Vázquez, M. I., Swiegers, J. H., Pretorius, I. S., Gómez-Puertas, P., Hegardt, F. G., Asins, G. & Serra, D., 6 Aug 2004, In : Journal of Biological Chemistry. 279, 32, p. 33899-33908 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Carnitine O-Acetyltransferase
Protein Engineering
Proteins
Acetylcarnitine
Substrates

Variation in 4-mercapto-4-methyl-pentan-2-one release by Saccharomyces cerevisiae commercial wine strains

Howell, K. S., Swiegers, J. H., Elsey, G. M., Siebert, T. E., Bartowsky, E. J., Fleet, G. H., Pretorius, I. S. & De Barros Lopes, M. A., 15 Nov 2004, In : FEMS Microbiology Letters. 240, 2, p. 125-129 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Saccharomyces cerevisiae
Yeasts
Fermentation
Vitis
2003

Cellular Differentiation in Response to Nutrient Availability: The Repressor of Meiosis, Rme1p, Positively Regulates Invasive Growth in Saccharomyces cerevisiae

Van Dyk, D., Hansson, G., Pretorius, I. S. & Bauer, F. F., Nov 2003, In : Genetics. 165, 3, p. 1045-1058 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Meiosis
Saccharomyces cerevisiae
Food
Growth
Haploidy

Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts

Cordero Otero, R. R., Ubeda Iranzo, J. F., Briones-Perez, A. I., Potgieter, N., Villena, M. A., Pretorius, I. S. & Van Rensburg, P., Sep 2003, In : Journal of Food Science. 68, 8, p. 2564-2569 6 p.

Research output: Contribution to journalArticleResearchpeer-review

beta-Glucosidase
Wine
beta-glucosidase
wines
Yeasts

Enhancing volatile phenol concentrations in wine by expressing various phenolic acid decarboxylase genes in Saccharomyces cerevisiae

Smit, A., Cordero Otero, R. R., Lambrechts, M. G., Pretorius, I. S. & Van Rensburg, P., 13 Aug 2003, In : Journal of Agricultural and Food Chemistry. 51, 17, p. 4909-4915 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Phenol
phenolic acids
Yeast
phenol

Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters

Verstrepen, K. J., Van Laere, S. D. M., Vanderhaegen, B. M. P., Derdelinckx, G., Dufour, J. P., Pretorius, I. S., Winderickx, J., Thevelein, J. M. & Delvaux, F. R., 1 Sep 2003, In : Applied and Environmental Microbiology. 69, 9, p. 5228-5237 10 p.

Research output: Contribution to journalArticleResearchpeer-review

acetyltransferases
Acetyltransferases
ester
yeast
alcohol

Expression of the Aspergillus niger glucose oxidase gene in Saccharomyces cerevisiae and its potential applications in wine production

Malherbe, D. F., Du Toit, M., Cordero Otero, R. R., Van Rensburg, P. & Pretorius, I. S., Jun 2003, In : Applied Microbiology and Biotechnology. 61, 5-6, p. 502-511 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucose Oxidase
Glucose oxidase
Wine
Aspergillus niger
Aspergillus

Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol

Becker, J. V. W., Armstrong, G. O., van der Merwe, M. J., Lambrechts, M. G., Vivier, M. A. & Pretorius, I. S., Oct 2003, In : FEMS Yeast Research. 4, 1, p. 79-85 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Metabolic Engineering
Wine
Saccharomyces cerevisiae
Antioxidants
Vitis

Mss11p is a transcription factor regulating pseudohyphal differentiation, invasive growth and starch metabolism in Saccharomyces cerevisiae in response to nutrient availability

Gagiano, M., Bester, M., Van Dyk, D., Franken, J., Bauer, F. F. & Pretorius, I. S., 2003, In : Molecular Microbiology. 47, 1, p. 119-134 16 p.

Research output: Contribution to journalArticleResearchpeer-review

Starch
Saccharomyces cerevisiae
Transcription Factors
Food
Transcriptional Activation

Overexpression, secretion and antifungal activity of the Saccharomyces cerevisiae chitinase

Carstens, M., Vivier, M. A., Van Rensburg, P. & Pretorius, I. S., 2003, In : Annals of Microbiology. 53, 1, p. 15-28 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Chitinases
Saccharomyces cerevisiae
Yeasts
Pesticides
Agricultural Crops

Starch Fermentation by Recombinant Saccharomyces cerevisiae Strains Expressing the α-Amylase and Glucoamylase Genes from Lipomyces Kononenkoae and Saccharomycopsis fibuligera

Eksteen, J. M., Van Rensburg, P., Cordero Otero, R. R. & Pretorius, I. S., 20 Dec 2003, In : Biotechnology and Bioengineering. 84, 6, p. 639-646 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Saccharomycopsis
Glucan 1,4-alpha-Glucosidase
Amylases
alpha-Amylases

The connection between yeast and alcohol reduction in wine: managing the double-edged sword of bottled sunshine

de Barros Lopes, M., Eglinton, J., Henschke, P., Høj, P. & Pretorius, I., 2003, In : Australian and New Zealand wine industry journal. 18, 4, p. 17-22 6 p.

Research output: Contribution to journalArticleResearch

The Saccharomyces cerevisiae alcohol acetyl transferase gene ATF1 is a target of the cAMP/PKA and FGM nutrient-signalling pathways

Verstrepen, K. J., Derdelinckx, G., Dufour, J. P., Winderickx, J., Pretorius, I. S., Thevelein, J. M. & Delvaux, F. R., Dec 2003, In : FEMS Yeast Research. 4, 3, p. 285-296 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Transferases
Cyclic AMP-Dependent Protein Kinases
Saccharomyces cerevisiae
Esters
Alcohols

The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene, confers antifungal activity against Botrytis cinerea to transgenic tobacco

Carstens, M., Vivier, M. A. & Pretorius, I. S., Aug 2003, In : Transgenic Research. 12, 4, p. 497-508 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Botrytis
Chitinases
chitinase
Botrytis cinerea
Tobacco
2002

Carnitine biosynthesis in Neurospora crassa: Identification of a cDNA coding for ε-N-trimethyllysine hydroxylase and its functional expression in Saccharomyces cerevisiae

Swiegers, J. H., Vaz, F. M., Pretorius, I. S., Wanders, R. J. A. & Bauer, F. F., 23 Apr 2002, In : FEMS Microbiology Letters. 210, 1, p. 19-23 5 p.

Research output: Contribution to journalArticleResearchpeer-review

epsilon-N-trimethyllysine hydroxylase
Neurospora crassa
Carnitine
Saccharomyces cerevisiae
Complementary DNA

Comparison of commercial enzymes for the processing of marula pulp, wine, and spirits

Fundira, M., Blom, M., Pretorius, I. S. & Van Rensburg, P., Aug 2002, In : Journal of Food Science. 67, 6, p. 2346-2351 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Anacardiaceae
Wine
pulp
wines
juices

Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae α-amylase in industrial strains of Saccharomyces cerevisiae

Gundllapalli Moses, S. B., Cordero Otero, R. R., La Grange, D. C., Van Rensburg, P. & Pretorius, I. S., 2002, In : Biotechnology Letters. 24, 8, p. 651-656 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Amylases
alpha-Amylases
Starch
Yeast

Identification and characterization of Saccharomyces cerevisiae and Saccharomyces paradoxus strains isolated from Croatian vineyards

Redžepović, S., Orlić, S., Sikora, S., Majdak, A. & Pretorius, I. S., 2002, In : Letters in Applied Microbiology. 35, 4, p. 305-310 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
vineyard
Yeast
Saccharomyces cerevisiae
Vitis

Microbial cellulose utilization: fundamentals and biotechnology

Lynd, L. R., Weimer, P. J., Van Zyl, W. H. & Pretorius, I. S., Sep 2002, In : Microbiology and Molecular Biology Reviews. 66, 3, p. 506-577 72 p.

Research output: Contribution to journalArticleResearchpeer-review

Biotechnology
Cellulose
Hydrolysis
Cellulase
Biomass

Selection of yeast starter culture strains for the production of marula fruit wines and distillates

Fundira, M., Blom, M., Pretorius, I. S. & Van Rensburg, P., 13 Mar 2002, In : Journal of Agricultural and Food Chemistry. 50, 6, p. 1535-1542 8 p.

Research output: Contribution to journalArticleResearchpeer-review

fruit wines
Anacardiaceae
distillates
Starters
Wine

The occurrence, control and esoteric effect of acetic acid bacteria in winemaking

Du Toit, W. J. & Pretorius, I. S., 2002, In : Annals of Microbiology. 52, 2, p. 155-179 25 p.

Research output: Contribution to journalArticleResearchpeer-review

Acetic Acid
Wine
Bacteria
Ethanol
Vitis
2001

Carnitine-dependent metabolic activities in Saccharomyces cerevisiae: Three carnitine acetyltransferases are essential in a carnitine-dependent strain

Swiegers, J. H., Dippenaar, N., Pretorius, I. S. & Bauer, F. F., 2001, In : Yeast. 18, 7, p. 585-595 11 p.

Research output: Contribution to journalArticleResearchpeer-review

carnitine acetyltransferase
Carnitine O-Acetyltransferase
Carnitine
carnitine
Yeast

Degradation of Xylan to D-Xylose by Recombinant Saccharomyces cerevisiae Coexpressing the Aspergillus niger β-Xylosidase (xlnD) and the Trichoderma reesei Xylanase II (xyn2) Genes

La Grange, D. C., Pretorius, I. S., Claeyssens, M. & Van Zyl, W. H., Dec 2001, In : Applied and Environmental Microbiology. 67, 12, p. 5512-5519 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Endo-1,4-beta Xylanases
xylan 1,4-beta-xylosidase
Xylans
Trichoderma reesei
Trichoderma

Late fermentation expression of FLO1 in Saccharomyces cerevisiae

Verstrepen, K. J., Derdelinckx, G., Delvaux, F. R., Winderickx, J., Thevelein, J. M., Bauer, F. F. & Pretorius, I. S., 2001, In : Journal of the American Society of Brewing Chemists. 59, 2, p. 69-76 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Flocculation
flocculation
Fermentation
Saccharomyces cerevisiae
fermentation

Wine and health

Armstrong, G. O., Lambrechts, M. G., Mansvelt, E. P. G., Van Velden, D. P. & Pretorius, I. S., 2001, In : South African Journal of Science. 97, 7-8, p. 279-282 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
wines
Health
drinking
Drinking
2000

Coexpression of the Bacillus pumilus β-xylosidase (xynB) gene with the Trichoderma reesei β-xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae

La Grange, D. C., Claeyssens, M., Pretorius, I. S. & Van Zyl, W. H., 2000, In : Applied Microbiology and Biotechnology. 54, 2, p. 195-200 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Endo-1,4-beta Xylanases
Trichoderma
Bacilli
Yeast
Saccharomyces cerevisiae

Effect of increased yeast alcohol acetyltransferase activity on flavor profiles of wine and distillates

Lilly, M., Lambrechts, M. G. & Pretorius, I. S., Feb 2000, In : Applied and Environmental Microbiology. 66, 2, p. 744-753 10 p.

Research output: Contribution to journalArticleResearchpeer-review

distillates
acetyltransferases
Acetyltransferases
Wine
yeast

Electron microscopy of the K2 killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast

Vadasz, A. S., Jagganath, D. B., Pretorius, I. S. & Gupthar, A. S., 2000, In : Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 78, 2, p. 117-122 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Saccharomyces cerevisiae
Electron Microscopy
Yeasts
Methylene Blue

Selection and improvement of wine yeasts

Rainieri, S. & Pretorius, I. S., 2000, In : Annals of Microbiology. 50, 1, p. 15-31 17 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Yeasts
Saccharomyces cerevisiae
Genetic Techniques
Saccharomyces
1999

Divergent regulation of the evolutionarily closely related promoters of the Saccharomyces cerevisiae STA2 and MUC1 genes

Gagiano, M., Van Dyk, D., Bauer, F. F., Lambrechts, M. G. & Pretorius, I. S., Oct 1999, In : Journal of Bacteriology. 181, 20, p. 6497-6508 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces cerevisiae
Genes
Flocculation
Catabolite Repression
Glucan 1,4-alpha-Glucosidase

Functional analysis of multiple AUG codons in the transcripts of the STA2 glucoamylase gene from Saccharomyces cerevisiae

Vivier, M. A., Sollitti, P. & Pretorius, I. S., 1999, In : Molecular and General Genetics. 261, 1, p. 11-20 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Codon
Saccharomyces cerevisiae
Genes
Open Reading Frames

Msn1p/Mss10p, Mss11p and Muc1p/Flo11p are part of a signal transduction pathway downstream of Mep2p regulating invasive growth and pseudohyphal differentiation in Saccharomyces cerevisiae

Gagiano, M., Van Dyk, D., Bauer, F. F., Lambrechts, M. G. & Pretorius, I. S., 1999, In : Molecular Microbiology. 31, 1, p. 103-116 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces cerevisiae
Signal Transduction
Growth
MAP Kinase Signaling System
Starch

The development of bactericidal yeast strains by expressing the Pediococcus acidilactici pediocin gene (pedA) in Saccharomyces cerevisiae

Schoeman, H., Vivier, M. A., Du Toit, M., Dicks, L. M. T. & Pretorius, I. S., 15 Jun 1999, In : Yeast. 15, 8, p. 647-656 10 p.

Research output: Contribution to journalArticleResearchpeer-review

pediocins
Pediococcus acidilactici
Yeast
Saccharomyces cerevisiae
Genes
1998

Development of a polysaccharide degrading strain of Saccharomyces cerevisiae

Petersen, S. H., Van Zyl, W. H. & Pretorius, I. S., 1998, In : Biotechnology Techniques. 12, 8, p. 615-619 5 p.

Research output: Contribution to journalArticleResearchpeer-review

beta-Glucosidase
Yeast
Biomass
Polysaccharides
Saccharomyces cerevisiae

Engineering yeast for efficient cellulose degradation

Van Rensburg, P., Van Zyl, W. H. & Pretorius, I. S., 15 Jan 1998, In : Yeast. 14, 1, p. 67-76 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Cellulase
Cellulose
Yeast
Yeasts
Saccharomyces cerevisiae

Genetic and fermentation properties of the K2 killer yeast, Saccharomyces cerevisiae T206

Franken, D. B., Ariatti, M., Pretorius, I. S. & Gupthar, A. S., 1998, In : Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 73, 3, p. 263-269 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Fermentation
Saccharomyces cerevisiae
Yeasts
RNA
Chromosomes, Human, Pair 13