Sakkie Pretorius

Professor

  • 12226 Citations
  • 54 h-Index
1983 …2020
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Research Outputs 1983 2019

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Article
1998

Identification of a functional TATA element in the STA2 glucoamylase gene promoter from Saccharomyces cerevisiae

Vivier, M. A. & Pretorius, I. S., Jan 1998, In : Current Genetics. 33, 1, p. 10-15 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Saccharomyces cerevisiae
Genes
TATA Box
Yeasts
1997

Cloning of the Bacillus pumilus β-xylosidase gene (xynB) and its expression in Saccharomyces cerevisiae

La Grange, D. C., Pretorius, I. S. & Van Zyl, W. H., 1997, In : Applied Microbiology and Biotechnology. 47, 3, p. 262-266 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Cloning
Bacilli
Yeast
Saccharomyces cerevisiae
Organism Cloning

Cloning of two β-xylanase-encoding genes from Aspergillus niger and their expression in Saccharomyces cerevisiae

Luttig, M., Pretorius, I. S. & Van Zyl, W. H., 1997, In : Biotechnology Letters. 19, 5, p. 411-415 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Endo-1,4-beta Xylanases
Gene encoding
Aspergillus niger
Cloning
Aspergillus

MSS11, a novel yeast gene involved in the regulation of starch metabolism

Webber, A. L., Lambrechts, M. G. & Pretorius, I. S., 1997, In : Current Genetics. 32, 4, p. 260-266 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Starch
Yeasts
Genes
Saccharomyces cerevisiae
Endo-1,3(4)-beta-Glucanase
Glucan 1,3-beta-Glucosidase
Cellulase
Bacilli
Bacillus subtilis
1996

A multicopy suppressor gene, MSS10, restores STA2 expression in Saccharomyces cerevisiae strains containing the STA10 represser gene

Lambrechts, M. G., Sollitti, P., Murmur, J. & Pretorius, I. S., 1996, In : Current Genetics. 29, 6, p. 523-529 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Suppressor Genes
Saccharomyces cerevisiae
Glucan 1,4-alpha-Glucosidase
Genes
beta-Fructofuranosidase

Cloning, mapping and characterization of a genomic copy of the Lipomyces kononenkoae α-amylase-encoding gene (LKA1)

Steyn, A. J. C., Marmur, J. & Pretorius, I. S., Aug 1996, In : Yeast. 12, 10, p. 925-937 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Amylases
Gene encoding
alpha-Amylases
Cloning

Cloning and expression of the Clostridium thermosulfurogenes d-xylose isomerase gene (xylA) in Saccharomyces cerevlsiae

Moes, C. J., Pretorius, I. S. & Van Zyl, W. H., 1996, In : Biotechnology Letters. 18, 3, p. 269-274 6 p.

Research output: Contribution to journalArticleResearchpeer-review

xylose isomerase
Clostridium
Saccharomyces
Xylose
Cloning

Cloning and expression of the α-L-arabinofuranosidase gene (ABF2) of Aspergillus niger in Saccharomyces cerevisiae

Crous, J. M., Pretorius, I. S. & Van Zyl, W. H., 1996, In : Applied Microbiology and Biotechnology. 46, 3, p. 256-260 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Aspergillus niger
Cloning
Aspergillus
Yeast
Saccharomyces cerevisiae

Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-β-1,4-glucanase gene in Saccharomyces cerevisiae

Van Rensburg, P., Van Zyl, W. H. & Pretorius, I. S., Aug 1996, In : Current Genetics. 30, 3, p. 246-250 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Cellulose 1,4-beta-Cellobiosidase
Phanerochaete
Cellulase
Saccharomyces cerevisiae
Genes

Expression of a Trichoderma reesei β;-xylanase gene (XYN2) in Saccharomyces cerevisiae

La Grange, D. C., Pretorius, I. S. & Van Zyl, W. H., Mar 1996, In : Applied and Environmental Microbiology. 62, 3, p. 1036-1044 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Endo-1,4-beta Xylanases
Trichoderma reesei
Trichoderma
xylanases
Saccharomyces cerevisiae

MUC1, a mucin-like protein that is regulated by MSS10, is critical for pseudohyphal differentiation in yeast

Lambrechts, M. G., Bauer, F. F., Marmur, J. & Pretorius, I. S., 6 Aug 1996, In : Proceedings of the National Academy of Sciences of the United States of America. 93, 16, p. 8419-8424 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Mucins
Yeasts
Diploidy
Starch
Saccharomyces cerevisiae
1995

Characterization of a novel α-amylase from Lipomyces kononenkoae and expression of its gene (LKA1) in Saccharomyces cerevisiae

Steyn, A. J. C. & Pretorius, I. S., Nov 1995, In : Current Genetics. 28, 6, p. 526-533 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
alpha-Amylases
Glycosylation
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae

Cloning, sequence analysis and expression in yeasts of a cDNA containing a Lipomyces kononenkoae α-amylase-encoding gene

Steyn, A. J. C., Marmur, J. & Pretorius, I. S., 1 Dec 1995, In : Gene. 166, 1, p. 65-71 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
alpha-Amylases
Sequence Analysis
Organism Cloning
Complementary DNA

Cloning and expression of an Aspergillus kawachii endo-1,4-β-xylanase gene in Saccharomyces cerevisiae

Crous, J. M., Pretorius, I. S. & van Zyl, W. H., Oct 1995, In : Current Genetics. 28, 5, p. 467-473 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Endo-1,4-beta Xylanases
Aspergillus
Saccharomyces cerevisiae
Organism Cloning
Genes

Expression of human p450c17 as an export protein in saccharomyces cerevisiae

Swart, A. C., Swart, P., Roux, S. P., van der Merwe, K. J., Pretorius, I. S. & Steyn, A. J. C., 1995, In : Endocrine Research. 21, 1-2, p. 289-295 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces cerevisiae Proteins
Yeasts
Adrenal Cortex
Steroid 21-Hydroxylase
Reading Frames

Expression of the Ruminococcus flavefaciens cellodextrinase gene in Saccharomyces cerevisiae

van Rensburg, P., van Zyl, W. H. & Pretorius, I. S., May 1995, In : Biotechnology Letters. 17, 5, p. 481-486 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Ruminococcus
Yeast
Saccharomyces cerevisiae
Genes
Yeasts

One-step enzymatic hydrolysis of starch using a recombinant strain of Saccharomyces cerevisiae producing α-amylase, glucoamylase and pullulanase

Janse, B. J. H. & Pretorius, I. S., Mar 1995, In : Applied Microbiology and Biotechnology. 42, 6, p. 878-883 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
alpha-Amylases
Starch
Saccharomyces cerevisiae
Hydrolysis

The S1, S2 and SGA1 ancestral genes for the STA glucoamylase genes all map to chromosome IX in Saccharomyces cerevisiae

Lambrechts, M. G., Pretorius, I. S., Marmur, J. & Sollitti, P., 1995, In : Yeast. 11, 8, p. 783-787 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Chromosomes
Yeast
Saccharomyces cerevisiae
Gene encoding
1994

Expression of the Butyrivibrio fibrisolvens endo-β-1,4-glucanase gene together with the Erwinia pectate lyase and polygalacturonase genes in Saccharomyces cerevisiae

van Rensburg, P., van Zyl, W. H. & Pretorius, I. S., Dec 1994, In : Current Genetics. 27, 1, p. 17-22 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Erwinia
Polygalacturonase
Cellulase
Saccharomyces cerevisiae
Yeasts

Multiple positive and negative cis-acting elements of the STA2 gene regulate glucoamylase synthesis in Saccharomyces cerevisiae

Lambrechts, M. G., Pretorius, I. S., D'Aguanno, V. S., Sollitti, P. & Marmur, J., 2 Sep 1994, In : Gene. 146, 2, p. 137-144 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Saccharomyces cerevisiae
Genes
Genetic Promoter Regions
Transcriptional Activation

Optimization of α-amylase and glucoamylase production by recombinant strains of Saccharomyces cerevisiae

D'Aguanno, V. S. & Pretorius, I. S., Jul 1994, In : Biotechnology Letters. 16, 7, p. 727-732 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Amylases
alpha-Amylases
Yeast
Saccharomyces cerevisiae
1993
Pectobacterium carotovorum
Polygalacturonase
Saccharomyces cerevisiae
Yeasts
Mating Factor
Pectobacterium chrysanthemi
Polygalacturonase
Gene encoding
Yeast
Saccharomyces cerevisiae

Expression of the Klebsiella pneumoniae pullulanase-encoding gene in Saccharomyces cerevisiae

Janse, B. J. H. & Pretorius, I. S., Jul 1993, In : Current Genetics. 24, 1-2, p. 32-37 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Klebsiella pneumoniae
Saccharomyces cerevisiae
Yeasts
Mating Factor
Genes

Regional sequence homologies in starch-degrading enzymes

Janse, B. J. H., Steyn, A. J. C. & Pretorius, I. S., Nov 1993, In : Current Genetics. 24, 5, p. 400-407 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Sequence Homology
Starch
alpha-Amylases
Enzymes
1992

Synthesis and secretion of an Erwinia chrysanthemi pectate lyase in Saccharomyces cerevisiae regulated by different combinations of bacterial and yeast promoter and signal sequences

Laing, E. & Pretorius, I. S., 2 Nov 1992, In : Gene. 121, 1, p. 35-45 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Pectobacterium chrysanthemi
Protein Sorting Signals
Saccharomyces cerevisiae
Yeasts
Mating Factor

The value of electrophoretic fingerprinting and karyotyping in wine yeast breeding programmes

van der Westhuizen, T. J. & Pretorius, I. S., May 1992, In : Antonie van Leeuwenhoek. 61, 4, p. 249-257 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Karyotyping
Wine
Breeding
Yeasts
DNA Fingerprinting
1991

Co-expression of a Saccharomyces diastaticus glucoamylase-encoding gene and a Bacillus amyloliquefaciens α-amylase-encoding gene in Saccharomyces cerevisiae

Steyn, A. J. C. & Pretorius, I. S., 1991, In : Gene. 100, C, p. 85-93 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Saccharomyces
alpha-Amylases
Saccharomyces cerevisiae
Plasmids

Primary structure and regulation of a glucoamylase-encoding gene (STA2) in Saccharomyces diastaticus

Lambrechts, M. G., Pretorius, I. S., Sollitti, P. & Marmur, J., 1991, In : Gene. 100, C, p. 95-103 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Saccharomyces
Genes
Open Reading Frames
Repressor Proteins

The glucoamylase multigene family in saccharomyces cerevisiae var. diastaticus: An overview

Pretorius, I. S., Lambrechts, M. G., Marmur, J. & Mattoon, J. R., 1991, In : Critical Reviews in Biochemistry and Molecular Biology. 26, 1, p. 53-76 24 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Multigene Family
Yeast
Saccharomyces cerevisiae
Genes
1990

A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome

Wingfield, B. D., Southgate, V. J., Pretorius, I. S. & Van Vuuren, H. J. J., 1990, In : Yeast. 6, 2, p. 159-169 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Double-Stranded RNA
RNA
Yeast
Saccharomyces cerevisiae
Genes

K3 killer yeast is a mutant K2 killer yeast

Wingfield, B. D., Van Der Meer, L. J., Pretorius, I. S. & Van Vuuren, H. J. J., 1990, In : Mycological Research. 94, 7, p. 901-906 6 p.

Research output: Contribution to journalArticleResearchpeer-review

homology
toxin
yeast
Saccharomyces cerevisiae
Yeasts
1988

Expression of a Bacillus α-amylase gene in yeast

Pretorius, I. S., Laing, E., Pretorius, G. H. J. & Marmu, J., Jul 1988, In : Current Genetics. 14, 1, p. 1-8 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
alpha-Amylases
Bacillus subtilis
Bacillus
Yeasts

Localization of yeast glucoamylase genes by PFGE and OFAGE

Pretorius, I. S. & Marmu, J., Jul 1988, In : Current Genetics. 14, 1, p. 9-13 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Pulsed Field Gel Electrophoresis
Electrophoresis
Yeasts
Gels
1987

Plasmids in Leuconostoc oenos

Janse, B. J. H., Wingfield, B. D., Pretorius, I. S. & van Vuuren, H. J. J., 1987, In : Plasmid. 17, 2, p. 173-175 3 p.

Research output: Contribution to journalArticleResearchpeer-review

Leuconostoc
Plasmids
DNA
1986

Identification and physical characterization of yeast glucoamylase structural genes

Pretorius, I. S., Chow, T. & Marmur, J., Apr 1986, In : MGG Molecular & General Genetics. 203, 1, p. 36-41 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Yeasts
Genes
Sequence Homology
Saccharomyces

Molecular cloning and characterization of the STA2 glucoamylase gene of Saccharomyces diastaticus

Pretorius, I. S., Chow, T., Modena, D. & Marmur, J., Apr 1986, In : MGG Molecular & General Genetics. 203, 1, p. 29-35 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucan 1,4-alpha-Glucosidase
Saccharomyces
Molecular Cloning
Genes
Yeasts

Numerical taxonomy of α‐amylase producing Bacillus species

Pretorius, I. S., Kock, M. J. D., Britz, T. J., Potgieter, H. J. & Lategan, P. M., 1986, In : Journal of Applied Bacteriology. 60, 4, p. 351-360 10 p.

Research output: Contribution to journalArticleResearchpeer-review

alpha-Amylases
Bacillus
Amylases
Bacillus subtilis

Transcriptional control of glucoamylase synthesis in vegetatively growing and sporulating Saccharomyces species

Pretorius, I. S., Modena, D., Vanoni, M., Englard, S. & Marmur, J., 1986, In : Molecular and Cellular Biology. 6, 9, p. 3034-3041 8 p.

Research output: Contribution to journalArticleResearchpeer-review

1983

Nutritional, temperature, pH, and oxygen requirements of Candida wickerhamii

Kilian, S. G., Prior, B. A., Pretorius, I. S., du Preez, J. C., Venter, J. J. & Potgieter, H. J., Nov 1983, In : European Journal of Applied Microbiology and Biotechnology. 17, 6, p. 334-338 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Candida
Oxygen
Cellobiose
Temperature
Ethanol