Domesticating WO phage as a controllable transformation vector for Wolbachia engineering

  • Jaschke, Paul (Primary Chief Investigator)
  • Logel, Dominic (Student)
  • Edwards, Owain (Co-Investigator)

Project: Other

Project Details


Wolbachia has well-established capabilities as a gene drive and arbovirus resistance vector, however significant impediments exist for transformation and engineering of the bacteria. Recent research has identified the Wolbachia bacteriophage, WO, as a likely transformation platform for Wolbachia but issues remain, such as integrase site verification, selectable markers, and standardisation (1,2). This project will address these challenges by engineering a controllable variant of the bacteriophage in the Aedes aegypti endosymbiont, wAlbB that can be assembled and packaged in vitro and delivered to transform naïve hosts with any given gene cargo
Effective start/end date1/01/1817/07/19