Genomics has led to the discovery of both the fine and gross characteristics of specific microbial physiologies. This project aims to take the genetic diversity inherent in microbial systems and exploit it for biotechnology applications, using the cutting-edge facilities available for synthetic biology. The acquisition of a long read sequencing platform, droplet digital PCR machine, DNA library system and small scale bioreactor will address the limitations of short read sequencing, large fragment cloning and gene expression technologies, currently creating bottlenecks for synthetic biologists.