β-adrenoceptor agonists interfere with glucocorticoid receptor DNA binding in rat lung

Matthew J. Peters, Ian M. Adcock*, Carolanne R. Brown, Peter J. Barnes

*Corresponding author for this work

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Inhaled β2-adrenoceptor agonists are the most effective bronchodilator treatment in asthma, yet paradoxically high doses may be associated with increased asthma morbidity and mortality. Steroids are the most effective therapy in controlling asthmatic inflammation and act by binding to specific sequences of DNA (GRE), thus modulating gene transcription. We report that in rat lung, the β2-adrenoceptor agonists, salbutamol and fenoterol, decrease the binding of glucocorticoid receptors to GRE, by 46 ± 4% although it has no effect on the affinity or number of glucocorticoid receptors. The inhibition of GRE binding by salbutamol is concentration-dependent, can be blocked by propranolol and is seen following forskolin treatment. This effect appears to be due to an interaction between the glucocorticoid receptor and the transcription factor, cAMP response element binding protein (CREB), which is activated by high concentrations of β2-adrenoceptors agonists. We suggest that by this mechanism high doses of inhaled β2-adrenoceptor agonists may inhibit the anti-inflammatory effects of endogenous glucocorticoids and exogenous corticosteroids used for asthma therapy.

Original languageEnglish
Pages (from-to)275-281
Number of pages7
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume289
Issue number2
DOIs
Publication statusPublished - 28 Apr 1995
Externally publishedYes

Keywords

  • (Rat)
  • Dexamethasone
  • DNA binding
  • Lung
  • β-adrenoceptor agonists

Fingerprint Dive into the research topics of 'β-adrenoceptor agonists interfere with glucocorticoid receptor DNA binding in rat lung'. Together they form a unique fingerprint.

  • Cite this