Abstract
شپشک آردآلود پنبه (Hemiptera: Pseudococcidae)Tinsely Phenacoccus solenopsis یکی از آفات مهم بسیاری از گیاهان در اکوسیستمهای کشاورزی و فضاهای سبز شهری میباشد. در این مطالعه، امکان به کارگیری سه جدایه ایرانی از قارچ Akanthomyces lecanii (جدایههای PAL6، PAL7 و PAL8) و یک جدایه ایرانی از گونه A. muscarius (جدایه GAM5) در کنترل میکروبی این آفت در شرایط گلخانهای و روی گیاه حسن یوسف (به عنوان میزبان گیاهی شپشک) مورد ارزیابی قرار گرفت. برای این منظور، گیاهان آلوده به پنج پورههای سن آخر شپشک توسط غلظتهای مختلف (104، 105، 106، 107 و 108 کنیدی در هر میلیلیتر) هر جدایه تیمار شده و میزان مرگ و میر شپشکها تا هشت روز و به صورت روزانه ثبت شد. میزان مرگ و میر از 3/14 درصد (در جدایه PAL7) تا 79/1 درصد (در جدایه PAL8) متغیر بود. بیشترین میزان مرگ و میر در غلظت 108 کنیدی در هر میلیلیتر بیمارگرهای مزبور دیده شد. بیشترین مرگ و میر ناشی از بیماریزایی جدایهها در روز ششم پس از تیمار ثبت شد. به طور کلی، سوسپانسیون کنیدی جدایه PAL7 قارچ A. lecanii با غلظت 108 کنیدی در هر میلیلیتر بهترین گزینه بین بیمارگرهای مورد مطالعه برای کنترل میکروبی شپشک آردآلود پنبه بود که در شش روز پس از تیمار موجب 17/56 درصد مرگ و میر در جمعیت این آفت شد. از نتایج این تحقیق میتوان برای توسعه کنترل میکروبی شپشک آرد آلود پنبه به ویژه در محیطهای گلخانهای استفاده نمود.
The cotton mealybug, Phenacoccus solenopsis Tinsely (Hemiptera: Pseudococcidae), is an important pest of many plants in agroecosystems and urban green landscapes. In this study, potential of three isolates of Akanthomyces lecanii, isolates PAL6, PAL7, and PAL8, and an isolate of A. muscarius, isolate GAM5, to microbial biocontrol of this pest on Coleus blumei, as the pest host plant, were investigated under greenhouse conditions. The infested host plants to five last instar nymphs were treated using different conidial suspension concentrations (104,105, 106, 107, and 108 conidia/mL) of each isolate and the mealybug mortalities were recorded every day, for eight days. The mortalities were varied from 14.3% (isolate PAL7) to 1.79% (isolate PAL8). The highest mortality was observed in concentration of 108 conidia/mL of the pathogens. The highest mortality caused by the pathogens was recorded six days after treatment. Totally, conidial suspension concentration of 108 conidia/mL from A. lecanii PAL7 was the best candidate for microbial biocontrol of the mealybug among the pathogens causing 56.17% mortality in the pest population six days after treatment. The results can be used for developing microbial biocontrol of the cotton mealybug especially in greenhouse conditions.
The cotton mealybug, Phenacoccus solenopsis Tinsely (Hemiptera: Pseudococcidae), is an important pest of many plants in agroecosystems and urban green landscapes. In this study, potential of three isolates of Akanthomyces lecanii, isolates PAL6, PAL7, and PAL8, and an isolate of A. muscarius, isolate GAM5, to microbial biocontrol of this pest on Coleus blumei, as the pest host plant, were investigated under greenhouse conditions. The infested host plants to five last instar nymphs were treated using different conidial suspension concentrations (104,105, 106, 107, and 108 conidia/mL) of each isolate and the mealybug mortalities were recorded every day, for eight days. The mortalities were varied from 14.3% (isolate PAL7) to 1.79% (isolate PAL8). The highest mortality was observed in concentration of 108 conidia/mL of the pathogens. The highest mortality caused by the pathogens was recorded six days after treatment. Totally, conidial suspension concentration of 108 conidia/mL from A. lecanii PAL7 was the best candidate for microbial biocontrol of the mealybug among the pathogens causing 56.17% mortality in the pest population six days after treatment. The results can be used for developing microbial biocontrol of the cotton mealybug especially in greenhouse conditions.
| Translated title of the contribution | Potential of some Irianian isolates of Akanthomyces for microbial control of cotton mealybug, Phenacoccus solenopsis, under greenhouse conditions |
|---|---|
| Original language | Persian |
| Pages (from-to) | 57-70 |
| Number of pages | 14 |
| Journal | Plant Pest Research |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Dec 2022 |
| Externally published | Yes |
Bibliographical note
Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.Keywords
- Greenhouse pests
- IPM
- microbiological control
- pathogenesis