一种灵敏检测节球藻毒素的电化学适体传感器的制备方法

本发明涉及一种用于节球藻毒素检测的电化学适体传感器制备方法，包括以下步骤：采用煅烧法和水热法分别制备功能化含碳过渡双金属氧化物纳米材料和钙钛矿。利用氧化还原法制备贵金属颗粒，并原为生长在钙钛矿上，并通过化学键键合得到贵金属@钙钛矿/功能化含碳双过渡金属氧化物纳米材料。体外合成双嵌段Y型探针后，依次在电极表面滴加cDNA2和双嵌段Y型探针，制得双嵌段Y型探针/cDNA2/贵金属@钙钛矿/功能化含碳双过渡金属氧化物纳米材料/金电极。以双嵌段Y型探针/cDNA2/贵金属@钙钛矿/功能化含碳双过渡金属氧化物纳米材料/金电极为工作电极，银|氯化银电极为参比电极，铂丝电极为对电极，通过峰电流信号变化指示样品中节球藻毒素的含量，得到了用于节球藻毒素检测的电化学适体传感器。与传统检测节球藻毒素含量的仪器相比，所制备的电化学适体传感器具有操作简单、价格低廉、快速便携的优势。

The invention relates to a preparation method of an electrochemical aptamer sensor for nodularin detection. The preparation method comprises the following steps: respectively preparing a functionalized carbon-containing transition bimetallic oxide nano material and perovskite by adopting a calcination method and a hydrothermal method; precious metal particles are prepared through an oxidation-reduction method and originally grow on perovskite, and the precious metal and perovskite/functional carbon-containing double-transition metal oxide nano material is obtained through chemical bond bonding. After a double-block Y-type probe is synthesized in vitro, cDNA2 and the double-block Y-type probe are sequentially dropwise added to the surface of the electrode, and the double-block Y-type probe/cDNA2/precious metal perovskite/functionalized carbon-containing double transition metal oxide nano material/gold electrode is prepared. A double-block Y-type probe/cDNA2/precious metal at perovskite/functionalized carbon-containing double transition metal oxide nanomaterial/gold electrode is used as a working electrode, a silver-silver chloride electrode is used as a reference electrode, a platinum wire electrode is used as a counter electrode, and the content of nodularin in a sample is indicated through peak current signal change. The electrochemical aptamer sensor for nodularia toxin detection is obtained. Compared with a traditional instrument for detecting the content of nodularin, the prepared electrochemical aptamer sensor has the advantages of simplicity in operation, low price, rapidness and portability.