Abstract
Objective: To explore the optimal technology of celastrol extraction and purification from C.anticulatus Thunb.
Methods: By comparing the extraction efficiency of refluxing extraction, supercritieal C02 extraction and ultrasound-assisted extraction, ultrasound-assisted extraction was chosen eventually and optimized by orthogonal experiments; silica-gel column chromatography process for purification was also investigated.
Results: The optimal extraction process consisted of a liquid-solid ratio of l0 ：1 （ v/w ）, 400 W of extracting power, 5 seconds of once radiated time and 40 minutes of extraction time.The content of celastrol was not less than 90%, when purified by silica-gel column chromatography process.
Conclusions: The application of ultrasonic method showed to be highly efficient in the extraction of celastrol, compared with classical methods ; silica-gel column chromatography process was simple and the purification degree was high.
Methods: By comparing the extraction efficiency of refluxing extraction, supercritieal C02 extraction and ultrasound-assisted extraction, ultrasound-assisted extraction was chosen eventually and optimized by orthogonal experiments; silica-gel column chromatography process for purification was also investigated.
Results: The optimal extraction process consisted of a liquid-solid ratio of l0 ：1 （ v/w ）, 400 W of extracting power, 5 seconds of once radiated time and 40 minutes of extraction time.The content of celastrol was not less than 90%, when purified by silica-gel column chromatography process.
Conclusions: The application of ultrasonic method showed to be highly efficient in the extraction of celastrol, compared with classical methods ; silica-gel column chromatography process was simple and the purification degree was high.
| Translated title of the contribution | Study on Extraction and Purification of Celastrol from C.Anticulatus Thunb |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | 48-50 |
| Number of pages | 3 |
| Journal | Journal of Liaoning University of Traditional Chinese Medicine |
| Volume | 2013 |
| Issue number | 6 |
| Publication status | Published - 2013 |
| Externally published | Yes |
Keywords
- celastrol
- ultrasound-assisted extraction
- Orthogonal experiment
- column chromatography