双层蛛丝蛋白血管支架的体外降解研究

The preparation of vascular scaffold becomes a necessary condition for cure for cardiovascular disease. In this paper electrospinning technique was used to prepare the (pNSR16/PCL/CS)/(pNSR16/PCL/Gt) bilayer vascular scaffold, and its degradation in vitro was studied after being soaked in phosphate buffer solution (pH=7.14) and multiple enzyme solution for different time. The changes of weight loss rate, water absorption rate, pH value of degradation solution, mechanical property and molecular weight were tested after sampling at 2nd, 4 th, 8 ht, 12 th week respectively, and at the same time the morphology was observed using scanning electron microscope. The results showed that the initial bending strength and molecular weight of (pNSR16/PCL/CS)/(pNSR16/PCL/Gt) bilayer vascular scaffold were greater than those of (PCL/CS)/(PCL/Gt), the degradation velocity and decreasement velocity of molecular weight and weight loss rate of the former were faster than the latter, and the addition of pNSR16 promoted the degradation of vascular scaffold. In the process of degradation, pH value of degradation solution was weakly acidic and neutral, the scaffold decreased rapidly in the prophase and showed stable trend in the late period. The degradation speed of scaffold in the enzyme solution was faster than it in the hydrolysis soultion.