200+ protein concentrations in healthy human blood plasma

targeted quantitative SRM SIS screening of chromosomes 18, 13, Y, and the mitochondrial chromosome encoded proteome

Arthur T. Kopylov, Elena A. Ponomarenko*, Ekaterina V. Ilgisonis, Mikhail A. Pyatnitskiy, Andrey V. Lisitsa, Ekaterina V. Poverennaya, Olga I. Kiseleva, Tatiana E. Farafonova, Olga V. Tikhonova, Maria G. Zavialova, Svetlana E. Novikova, Sergey A. Moshkovskii, Sergey P. Radko, Boris V. Morukov, Anatoly I. Grigoriev, Young-Ki Paik, Ghasem Hosseini Salekdeh, Andrea Urbani, Victor G. Zgoda, Alexander I. Archakov

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This work continues the series of the quantitative measurements of the proteins encoded by different chromosomes in the blood plasma of a healthy person. Selected Reaction Monitoring with Stable Isotope-labeled peptide Standards (SRM SIS) and a gene-centric approach, which is the basis for the implementation of the international Chromosome-centric Human Proteome Project (C-HPP), were applied for the quantitative measurement of proteins in human blood plasma. Analyses were carried out in the frame of C-HPP for each protein-coding gene of the four human chromosomes: 18, 13, Y, and mitochondrial. Concentrations of proteins encoded by 667 genes were measured in 54 blood plasma samples of the volunteers, whose health conditions were consistent with requirements for astronauts. The gene list included 276, 329, 47, and 15 genes of chromosomes 18, 13, Y, and the mitochondrial chromosome, respectively. This paper does not make claims about the detection of missing proteins. Only 205 proteins (30.7%) were detected in the samples. Of them, 84, 106, 10, and 5 belonged to chromosomes 18, 13, and Y and the mitochondrial chromosome, respectively. Each detected protein was found in at least one of the samples analyzed. The SRM SIS raw data are available in the ProteomeXchange repository (PXD004374, PASS01192).

Original languageEnglish
Pages (from-to)120-129
Number of pages10
JournalJournal of Proteome Research
Volume18
Issue number1
DOIs
Publication statusPublished - 4 Jan 2019

Keywords

  • plasma proteome
  • targeted proteomics
  • selected reaction monitoring (SRM SIS)
  • healthy human
  • Chromosome-centric Human Proteome Project (C-HPP)
  • Human Plasma Proteome Project (HPPP)

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