TY - JOUR
T1 - 3D printing of alginate/chitosan-based scaffold empowered by tyrosol-loaded niosome for wound healing applications
T2 - in vitro and in vivo performances
AU - Beram, Farzaneh Mahmoudi
AU - Ali, Saba Naeimaei
AU - Mesbahian, Ghazal
AU - Pashizeh, Fatemeh
AU - Keshvadi, Mohammadhosein
AU - Mashayekhi, Farzaneh
AU - Khodadadi, Behnoosh
AU - Bashiri, Zahra
AU - Moeinzadeh, Alaa
AU - Rezaei, Niloufar
AU - Namazifard, Saina
AU - Hossein-Khannazer, Nikoo
AU - Tavakkoli Yaraki, Mohammad
PY - 2024/3/18
Y1 - 2024/3/18
N2 - This study introduces a tyrosol-loaded niosome integrated into a chitosan-alginate scaffold (Nio-Tyro@CS-AL), employing advanced electrospinning and 3D printing techniques for wound healing applications. The niosomes, measuring 185.40 ± 6.40 nm with a polydispersity index of 0.168 ± 0.012, encapsulated tyrosol with an efficiency of 77.54 ± 1.25%. The scaffold’s microsized porous structure (600-900 μm) enhances water absorption, promoting cell adhesion, migration, and proliferation. Mechanical property assessments revealed the scaffold’s enhanced resilience, with niosomes increasing the compressive strength, modulus, and strain to failure, indicative of its suitability for wound healing. Controlled tyrosol release was demonstrated in vitro, essential for therapeutic efficacy. The scaffold exhibited significant antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus, with substantial biofilm inhibition and downregulation of bacterial genes (ndvb and icab). A wound healing assay highlighted a notable increase in MMP-2 and MMP-9 mRNA expression and the wound closure area (69.35 ± 2.21%) in HFF cells treated with Nio-Tyro@CS-AL. In vivo studies in mice confirmed the scaffold’s biocompatibility, showing no significant inflammatory response, hypertrophic scarring, or foreign body reaction. Histological evaluations revealed increased fibroblast and macrophage activity, enhanced re-epithelialization, and angiogenesis in wounds treated with Nio-Tyro@CS-AL, indicating effective tissue integration and repair. Overall, the Nio-Tyro@CS-AL scaffold presents a significant advancement in wound-healing materials, combining antibacterial properties with enhanced tissue regeneration, and holds promising potential for clinical applications in wound management.
AB - This study introduces a tyrosol-loaded niosome integrated into a chitosan-alginate scaffold (Nio-Tyro@CS-AL), employing advanced electrospinning and 3D printing techniques for wound healing applications. The niosomes, measuring 185.40 ± 6.40 nm with a polydispersity index of 0.168 ± 0.012, encapsulated tyrosol with an efficiency of 77.54 ± 1.25%. The scaffold’s microsized porous structure (600-900 μm) enhances water absorption, promoting cell adhesion, migration, and proliferation. Mechanical property assessments revealed the scaffold’s enhanced resilience, with niosomes increasing the compressive strength, modulus, and strain to failure, indicative of its suitability for wound healing. Controlled tyrosol release was demonstrated in vitro, essential for therapeutic efficacy. The scaffold exhibited significant antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus, with substantial biofilm inhibition and downregulation of bacterial genes (ndvb and icab). A wound healing assay highlighted a notable increase in MMP-2 and MMP-9 mRNA expression and the wound closure area (69.35 ± 2.21%) in HFF cells treated with Nio-Tyro@CS-AL. In vivo studies in mice confirmed the scaffold’s biocompatibility, showing no significant inflammatory response, hypertrophic scarring, or foreign body reaction. Histological evaluations revealed increased fibroblast and macrophage activity, enhanced re-epithelialization, and angiogenesis in wounds treated with Nio-Tyro@CS-AL, indicating effective tissue integration and repair. Overall, the Nio-Tyro@CS-AL scaffold presents a significant advancement in wound-healing materials, combining antibacterial properties with enhanced tissue regeneration, and holds promising potential for clinical applications in wound management.
KW - 3D printing
KW - antibacterial and antibiofilm activity
KW - chitosan-alginate scaffold
KW - tyrosol
KW - wound dressing
UR - http://www.scopus.com/inward/record.url?scp=85187188455&partnerID=8YFLogxK
U2 - 10.1021/acsabm.3c00814
DO - 10.1021/acsabm.3c00814
M3 - Article
C2 - 38442406
AN - SCOPUS:85187188455
SN - 2576-6422
VL - 7
SP - 1449
EP - 1468
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 3
ER -