A β-d-xylosidase from the thermophile Caldocellum saccharolyticum expressed in Escherichia coli

Ernst Lüthi, Peter L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The gene coding for a β-d-xylosidase (E.C. 3.2.1.37) of the thermophile Caldocellum saccharolyticum was isolated previously as part of a gene cluster which has been cloned in Escherichia coli. The enzyme characteristics were determined in E. coli using toluenized cell extracts. The pH optimum was 6.5 and temperature optimum 70°C. The enzyme was stable at 60°C and the half life at 80°C was 45 minutes. The temperature optimum and the temperature stability exceed those reported for other bacterial or fungal β-d-xylosidases. The enzyme showed no other detectable xylanolytic or cellulolytic enzyme activity.

Original languageEnglish
Pages (from-to)291-294
Number of pages4
JournalFEMS Microbiology Letters
Volume67
Issue number3
DOIs
Publication statusPublished - 1990
Externally publishedYes

Keywords

  • Caldocellum saccharolyticum
  • Escherichia coli
  • Thermophile
  • β-d-Xylosidase

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