Abstract
The gene coding for a β-d-xylosidase (E.C. 3.2.1.37) of the thermophile Caldocellum saccharolyticum was isolated previously as part of a gene cluster which has been cloned in Escherichia coli. The enzyme characteristics were determined in E. coli using toluenized cell extracts. The pH optimum was 6.5 and temperature optimum 70°C. The enzyme was stable at 60°C and the half life at 80°C was 45 minutes. The temperature optimum and the temperature stability exceed those reported for other bacterial or fungal β-d-xylosidases. The enzyme showed no other detectable xylanolytic or cellulolytic enzyme activity.
| Original language | English |
|---|---|
| Pages (from-to) | 291-294 |
| Number of pages | 4 |
| Journal | FEMS Microbiology Letters |
| Volume | 67 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1990 |
| Externally published | Yes |
Keywords
- Caldocellum saccharolyticum
- Escherichia coli
- Thermophile
- β-d-Xylosidase
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