A case-control study of the role of human papillomavirus in oesophageal squamous cell carcinoma in Australia

Surabhi S. Liyanage; Eva Segelov; Aisha Malik; Suzanne M. Garland; Sepehr N. Tabrizi; Eleanor Cummins; Holly Seale; Bayzidur Rahman; Aye Moa; Andrew P. Barbour; Philip J. Crowe; C. Raina Macintyre

doi:10.1155/2014/236482

A case-control study of the role of human papillomavirus in oesophageal squamous cell carcinoma in Australia

Surabhi S. Liyanage^*, Eva Segelov, Aisha Malik, Suzanne M. Garland, Sepehr N. Tabrizi, Eleanor Cummins, Holly Seale, Bayzidur Rahman, Aye Moa, Andrew P. Barbour, Philip J. Crowe, C. Raina Macintyre

^*Corresponding author for this work

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Abstract

Objective. We investigate the prevalence of human papillomavirus (HPV) in oesophageal squamous cell carcinoma (OSCC) tissues compared to oesophageal tissue from healthy controls, in an Australian cohort. Methods. We conducted a hospital-based case-control study of 99 patients with OSCC and 100 healthy controls to examine the presence of HPV DNA. Paraffin tissues were tested using the PapType high-risk HPV detection and genotyping kit and with INNO-LiPA HPV Genotyping Extra. The biopsy samples were tested for HPV using a PCR-ELISA method based on the L1 consensus primer set PGMY09-PGMY11. Results. HPV DNA of the oncogenic genotype 16 was detected in 1/99 case specimens, a rate of 1010 per 100,000 (95% CI: 30-5500). All control specimens were negative for HPV. Significantly higher rates of smoking, other aerodigestive cancers, and mortality were seen among cases than controls. A pooled analysis of this study and the only other Australian case-control study found that 9/321 cases and 0/155 controls were positive for HPV. The pooled odds ratio for HPV being a risk factor for OSCC was 9.35 (95% CI: 0.47-190.33). Conclusion. Our results suggest that in this multifactorial cancer HPV may be an additional risk factor; although a larger, better powered study is needed.

Original language	English
Article number	236482
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of Oncology
Volume	2014
DOIs	https://doi.org/10.1155/2014/236482
Publication status	Published - 2014
Externally published	Yes

Bibliographical note

Copyright the Author(s) 2014. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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10.1155/2014/236482Licence: CC BY

Publisher version (open access)Final published version, 1.22 MBLicence: CC BY

Cite this

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title = "A case-control study of the role of human papillomavirus in oesophageal squamous cell carcinoma in Australia",

abstract = "Objective. We investigate the prevalence of human papillomavirus (HPV) in oesophageal squamous cell carcinoma (OSCC) tissues compared to oesophageal tissue from healthy controls, in an Australian cohort. Methods. We conducted a hospital-based case-control study of 99 patients with OSCC and 100 healthy controls to examine the presence of HPV DNA. Paraffin tissues were tested using the PapType high-risk HPV detection and genotyping kit and with INNO-LiPA HPV Genotyping Extra. The biopsy samples were tested for HPV using a PCR-ELISA method based on the L1 consensus primer set PGMY09-PGMY11. Results. HPV DNA of the oncogenic genotype 16 was detected in 1/99 case specimens, a rate of 1010 per 100,000 (95% CI: 30-5500). All control specimens were negative for HPV. Significantly higher rates of smoking, other aerodigestive cancers, and mortality were seen among cases than controls. A pooled analysis of this study and the only other Australian case-control study found that 9/321 cases and 0/155 controls were positive for HPV. The pooled odds ratio for HPV being a risk factor for OSCC was 9.35 (95% CI: 0.47-190.33). Conclusion. Our results suggest that in this multifactorial cancer HPV may be an additional risk factor; although a larger, better powered study is needed.",

author = "Liyanage, {Surabhi S.} and Eva Segelov and Aisha Malik and Garland, {Suzanne M.} and Tabrizi, {Sepehr N.} and Eleanor Cummins and Holly Seale and Bayzidur Rahman and Aye Moa and Barbour, {Andrew P.} and Crowe, {Philip J.} and Macintyre, {C. Raina}",

note = "Copyright the Author(s) 2014. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

year = "2014",

doi = "10.1155/2014/236482",

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AU - Liyanage, Surabhi S.

AU - Segelov, Eva

AU - Malik, Aisha

AU - Garland, Suzanne M.

AU - Tabrizi, Sepehr N.

AU - Cummins, Eleanor

AU - Seale, Holly

AU - Rahman, Bayzidur

AU - Moa, Aye

AU - Barbour, Andrew P.

AU - Crowe, Philip J.

AU - Macintyre, C. Raina

N1 - Copyright the Author(s) 2014. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2014

Y1 - 2014

N2 - Objective. We investigate the prevalence of human papillomavirus (HPV) in oesophageal squamous cell carcinoma (OSCC) tissues compared to oesophageal tissue from healthy controls, in an Australian cohort. Methods. We conducted a hospital-based case-control study of 99 patients with OSCC and 100 healthy controls to examine the presence of HPV DNA. Paraffin tissues were tested using the PapType high-risk HPV detection and genotyping kit and with INNO-LiPA HPV Genotyping Extra. The biopsy samples were tested for HPV using a PCR-ELISA method based on the L1 consensus primer set PGMY09-PGMY11. Results. HPV DNA of the oncogenic genotype 16 was detected in 1/99 case specimens, a rate of 1010 per 100,000 (95% CI: 30-5500). All control specimens were negative for HPV. Significantly higher rates of smoking, other aerodigestive cancers, and mortality were seen among cases than controls. A pooled analysis of this study and the only other Australian case-control study found that 9/321 cases and 0/155 controls were positive for HPV. The pooled odds ratio for HPV being a risk factor for OSCC was 9.35 (95% CI: 0.47-190.33). Conclusion. Our results suggest that in this multifactorial cancer HPV may be an additional risk factor; although a larger, better powered study is needed.

AB - Objective. We investigate the prevalence of human papillomavirus (HPV) in oesophageal squamous cell carcinoma (OSCC) tissues compared to oesophageal tissue from healthy controls, in an Australian cohort. Methods. We conducted a hospital-based case-control study of 99 patients with OSCC and 100 healthy controls to examine the presence of HPV DNA. Paraffin tissues were tested using the PapType high-risk HPV detection and genotyping kit and with INNO-LiPA HPV Genotyping Extra. The biopsy samples were tested for HPV using a PCR-ELISA method based on the L1 consensus primer set PGMY09-PGMY11. Results. HPV DNA of the oncogenic genotype 16 was detected in 1/99 case specimens, a rate of 1010 per 100,000 (95% CI: 30-5500). All control specimens were negative for HPV. Significantly higher rates of smoking, other aerodigestive cancers, and mortality were seen among cases than controls. A pooled analysis of this study and the only other Australian case-control study found that 9/321 cases and 0/155 controls were positive for HPV. The pooled odds ratio for HPV being a risk factor for OSCC was 9.35 (95% CI: 0.47-190.33). Conclusion. Our results suggest that in this multifactorial cancer HPV may be an additional risk factor; although a larger, better powered study is needed.

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A case-control study of the role of human papillomavirus in oesophageal squamous cell carcinoma in Australia

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