A cell culture assay for the in vitro efficacy testing of amoebocidal compounds against Neoparamoeba pemaquidensis

Mark Powell*, Tim Green, Craig Plaisted

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The search for effective treatments against amoebic gill disease has to date relied upon the use of acute toxicity testing of treatments such as freshwater and oxidative chemicals in both fresh and sea water. The assays used are limited by the duration over which the assay can be conducted and since they rely upon isolated gill amoebae from the gills of moribund fish, the samples are contaminated heavily with fish mucus and gill epithelia. A toxicity assay was developed based upon the 7 day in vitro culture of gill isolated amoebae. The in vitro culture toxicity assay of amprolium, albendazole, toltrazuril and bithionol at a final concentration of 1 mg.l -1 indicated that amprolium had potential as an amoebostat and that bithionol was amoebocidal at 1 and 10 mg.l -1. The culture toxicity assay may be useful as a tool for the screening of potential amobostatic and amoebocidal drugs for the development as controls for amoebic gill disease.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalBulletin of the European Association of Fish Pathologists
Volume23
Issue number6
Publication statusPublished - 2003
Externally publishedYes

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