The search for effective treatments against amoebic gill disease has to date relied upon the use of acute toxicity testing of treatments such as freshwater and oxidative chemicals in both fresh and sea water. The assays used are limited by the duration over which the assay can be conducted and since they rely upon isolated gill amoebae from the gills of moribund fish, the samples are contaminated heavily with fish mucus and gill epithelia. A toxicity assay was developed based upon the 7 day in vitro culture of gill isolated amoebae. The in vitro culture toxicity assay of amprolium, albendazole, toltrazuril and bithionol at a final concentration of 1 mg.l -1 indicated that amprolium had potential as an amoebostat and that bithionol was amoebocidal at 1 and 10 mg.l -1. The culture toxicity assay may be useful as a tool for the screening of potential amobostatic and amoebocidal drugs for the development as controls for amoebic gill disease.
|Number of pages||6|
|Journal||Bulletin of the European Association of Fish Pathologists|
|Publication status||Published - 2003|