A comparison of honeybee (Apis mellifera) queen, worker and drone larvae by RNA-Seq

Xu Jiang He, Wu Jun Jiang, Mi Zhou, Andrew B. Barron, Zhi Jiang Zeng

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Honeybees (Apis mellifera) have haplodiploid sex determination: males develop from unfertilized eggs and females develop from fertilized ones. The differences in larval food also determine the development of females. Here we compared the total somatic gene expression profiles of 2-day and 4-day-old drone, queen and worker larvae by RNA-Seq. The results from a co-expression network analysis on all expressed genes showed that 2-day-old drone and worker larvae were closer in gene expression profiles than 2-day-old queen larvae. This indicated that for young larvae (2-day-old) environmental factors such as larval diet have a greater effect on gene expression profiles than ploidy or sex determination. Drones had the most distinct gene expression profiles at the 4-day larval stage, suggesting that haploidy, or sex dramatically affects the gene expression of honeybee larvae. Drone larvae showed fewer differences in gene expression profiles at the 2-day and 4-day time points than the worker and queen larval comparisons (598 against 1190 and 1181), suggesting a different pattern of gene expression regulation during the larval development of haploid males compared to diploid females. This study indicates that early in development the queen caste has the most distinct gene expression profile, perhaps reflecting the very rapid growth and morphological specialization of this caste compared to workers and drones. Later in development the haploid male drones have the most distinct gene expression profile, perhaps reflecting the influence of ploidy or sex determination on gene expression.

LanguageEnglish
Pages499-509
Number of pages11
JournalInsect Science
Volume26
Issue number3
Early online date15 Dec 2017
DOIs
Publication statusPublished - Jun 2019

Fingerprint

drones (insects)
honeybee
Bees
queen insects
Transcriptome
Apis mellifera
Gene expression
gene expression
honey bees
Larva
RNA
larva
larvae
Haploidy
sex determination
Ploidies
haploidy
Social Class
gender
ploidy

Keywords

  • caste differentiation
  • environmental factors
  • gene expression
  • haploid and diploid
  • honeybees
  • larval development

Cite this

He, Xu Jiang ; Jiang, Wu Jun ; Zhou, Mi ; Barron, Andrew B. ; Zeng, Zhi Jiang. / A comparison of honeybee (Apis mellifera) queen, worker and drone larvae by RNA-Seq. In: Insect Science. 2019 ; Vol. 26, No. 3. pp. 499-509.
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A comparison of honeybee (Apis mellifera) queen, worker and drone larvae by RNA-Seq. / He, Xu Jiang; Jiang, Wu Jun; Zhou, Mi; Barron, Andrew B.; Zeng, Zhi Jiang.

In: Insect Science, Vol. 26, No. 3, 06.2019, p. 499-509.

Research output: Contribution to journalArticleResearchpeer-review

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