A comparison of metaphase arresting agents and tritiated thymidine. Autoradiography in measurement of the rate of entry of cells into mitosis in the crypts of Lieberkühn of the rat.

Rufus M. Clarke

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Three methods of estimating cell production rate were used: the rate of accumulation of metaphases blocked by Colcemid, or by vinblastine, and the rate of increase of labelled nuclei after administration of tritiated thymidine. These rates were determined for three sites in the small intestine by counts made on whole micro‐dissected crypts, fixed at various times after the administration of the agents. Within the limits of error of the methods, the cell production rate per crypt was the same when measured by each method (35/hr), and showed a slight fall from the proximal to the distal end of the small intestine (36/hr to 33/hr). the advantages and limitations of each method are discussed.

LanguageEnglish
Pages263-272
Number of pages10
JournalCell and Tissue Kinetics
Volume4
Issue number3
DOIs
Publication statusPublished - May 1971

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Metaphase
Autoradiography
Mitosis
Thymidine
Small Intestine
Demecolcine
Vinblastine

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A comparison of metaphase arresting agents and tritiated thymidine. Autoradiography in measurement of the rate of entry of cells into mitosis in the crypts of Lieberkühn of the rat. / Clarke, Rufus M.

In: Cell and Tissue Kinetics, Vol. 4, No. 3, 05.1971, p. 263-272.

Research output: Contribution to journalArticleResearchpeer-review

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