A continuous-flow automated assay for iodometric estimation of hydroperoxides

Sian M. Thomas*, Wendy Jessup, Janusz M. Gebicki, Roger T. Dean

*Corresponding author for this work

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

An iodometric method for the analysis of hydroperoxides has been automated to allow analysis of aqueous biological samples (containing less than 20 mg/ml protein) and lipid hydroperoxide extracts. The evolution of triiodide ions is measured spectrophotometrically at 370 nm. Dependent on the type of sample, 30-60 samples can be analyzed per hour and the system allows detection of less than 100 pmol of peroxide. The assay is linear over a range of 100 pmol to 25 nmol. The sample volume used routinely was 80 μl.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalAnalytical Biochemistry
Volume176
Issue number2
DOIs
Publication statusPublished - 1 Feb 1989

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