A continuous-flow automated assay for iodometric estimation of hydroperoxides

Sian M. Thomas; Wendy Jessup; Janusz M. Gebicki; Roger T. Dean

doi:10.1016/0003-2697(89)90322-9

A continuous-flow automated assay for iodometric estimation of hydroperoxides

Sian M. Thomas^*, Wendy Jessup, Janusz M. Gebicki, Roger T. Dean

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

An iodometric method for the analysis of hydroperoxides has been automated to allow analysis of aqueous biological samples (containing less than 20 mg/ml protein) and lipid hydroperoxide extracts. The evolution of triiodide ions is measured spectrophotometrically at 370 nm. Dependent on the type of sample, 30-60 samples can be analyzed per hour and the system allows detection of less than 100 pmol of peroxide. The assay is linear over a range of 100 pmol to 25 nmol. The sample volume used routinely was 80 μl.

Original language	English
Pages (from-to)	353-359
Number of pages	7
Journal	Analytical Biochemistry
Volume	176
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(89)90322-9
Publication status	Published - 1 Feb 1989

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abstract = "An iodometric method for the analysis of hydroperoxides has been automated to allow analysis of aqueous biological samples (containing less than 20 mg/ml protein) and lipid hydroperoxide extracts. The evolution of triiodide ions is measured spectrophotometrically at 370 nm. Dependent on the type of sample, 30-60 samples can be analyzed per hour and the system allows detection of less than 100 pmol of peroxide. The assay is linear over a range of 100 pmol to 25 nmol. The sample volume used routinely was 80 μl.",

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AB - An iodometric method for the analysis of hydroperoxides has been automated to allow analysis of aqueous biological samples (containing less than 20 mg/ml protein) and lipid hydroperoxide extracts. The evolution of triiodide ions is measured spectrophotometrically at 370 nm. Dependent on the type of sample, 30-60 samples can be analyzed per hour and the system allows detection of less than 100 pmol of peroxide. The assay is linear over a range of 100 pmol to 25 nmol. The sample volume used routinely was 80 μl.

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A continuous-flow automated assay for iodometric estimation of hydroperoxides

Abstract

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