Abstract
Time-gated luminescence images were obtained by analog summation of a series of sequential images that were obtained with a cooled modified interline CCD camera, and a fluorescence microscope modified to use a UV LED for illumination. The interline CCD camera obtains an analog sum of a multi-frame image by not reading out the storage line after each frame is acquired; instead, the charges from the acquisition pixels are transferred to the storage pixels, which adds them to those previously stored; subsequently, the sum of the images is readout from the storage pixels and digitized. The length of the exposure is limited by the capacity of the storage pixels and the rate of generation of background (noise). Previously, the quality of the images obtained with the room temperature camera was degraded by the buildup of thermal noise. The interline transfer, electronically shuttered, cooled astronomy CCD camera, which was modified for analog summation rapidly produced low noise images; yet permitted long exposures. The past problems with lanthanide dyes of low extinction coefficients and equipment cost have now been solved.
Original language | English |
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Title of host publication | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X |
Subtitle of host publication | Proceedings of SPIE Vol. 8225 |
Editors | Daniel L. Farkas, Dan V. Nicolau, Robert C. Leif |
Place of Publication | Bellingham, Washington |
Publisher | SPIE |
Pages | 1-8 |
Number of pages | 8 |
Volume | 8225 |
ISBN (Print) | 9780819488688 |
DOIs | |
Publication status | Published - 2012 |
Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X - San Francisco, CA, United States Duration: 21 Jan 2012 → 23 Jan 2012 |
Other
Other | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X |
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Country/Territory | United States |
City | San Francisco, CA |
Period | 21/01/12 → 23/01/12 |