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Abstract
Rapid and accurate detection of nucleic acids plays a critical role in public health, food safety and environmental management. Clustered regularly interspaced short palindromic repeats powered electrochemical sensor (E-CRISPR) is attractive as a point-of-care (POC) testing platform to fulfil this purpose. Yet, bulk noble metals (e.g., gold and platinum) based electrodes that have been widely adopted in E-CRISPR suffer from limited analytical performance and high manufacturing costs. Here, to address this limitation, we present a carbon-based E-CRISPR modified with gold nanoparticles and MXene Ti3C2 (a class of two-dimensional transition metal carbide nanomaterials) that provides highly stable and sensitive transduction of CRISPR-Cas12a trans-cleavage activity. Through systematic evaluation and optimization, our AuNPs/MXene Ti3C2 based E-CRISPR achieve the quantification of human papillomavirus 18 (HPV-18) DNA with a wide range of concentrations from 10 pM to 500 nM with a detection limit of 1.95 pM. We further evaluate the selectivity, degradation resistance and detection capability of the developed sensor during long-term storage. Notably, AuNPs/MXene based E-CRISPR retain more than 70% of initial current after 2 months and deliver reliable analytical results that are unaffected over 42-day storage. Owing to its excellent biofouling-resistant and analytical performance and robust shelf life, our E-CRISPR sensor offers a universal, scalable and low-cost strategy for POC nucleic acid testing.
Original language | English |
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Article number | 133342 |
Pages (from-to) | 1-9 |
Number of pages | 9 |
Journal | Sensors and Actuators B: Chemical |
Volume | 380 |
DOIs | |
Publication status | Published - 1 Apr 2023 |
Keywords
- CRISPR diagnosis
- Electrochemical biosensor
- Gold nanoparticles
- MXene Ti₃C₂
- Human papillomavirus detection
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Dive into the research topics of 'A CRISPR-Cas12a powered electrochemical sensor based on gold nanoparticles and MXene composite for enhanced nucleic acid detection'. Together they form a unique fingerprint.Projects
- 1 Finished
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Cell-Sort MultiTool: a Novel Platform for Single-cell Bacteria Analysis
Li, M., Cain, A., Tang, S. & Goda, K.
5/08/20 → 4/08/23
Project: Research