A CRISPR-Cas12a powered electrochemical sensor based on gold nanoparticles and MXene composite for enhanced nucleic acid detection

Haowei Duan, Yizhou Wang, Shi-Yang Tang, Ting-Hui Xiao, Keisuke Goda, Ming Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Rapid and accurate detection of nucleic acids plays a critical role in public health, food safety and environmental management. Clustered regularly interspaced short palindromic repeats powered electrochemical sensor (E-CRISPR) is attractive as a point-of-care (POC) testing platform to fulfil this purpose. Yet, bulk noble metals (e.g., gold and platinum) based electrodes that have been widely adopted in E-CRISPR suffer from limited analytical performance and high manufacturing costs. Here, to address this limitation, we present a carbon-based E-CRISPR modified with gold nanoparticles and MXene Ti3C2 (a class of two-dimensional transition metal carbide nanomaterials) that provides highly stable and sensitive transduction of CRISPR-Cas12a trans-cleavage activity. Through systematic evaluation and optimization, our AuNPs/MXene Ti3C2 based E-CRISPR achieve the quantification of human papillomavirus 18 (HPV-18) DNA with a wide range of concentrations from 10 pM to 500 nM with a detection limit of 1.95 pM. We further evaluate the selectivity, degradation resistance and detection capability of the developed sensor during long-term storage. Notably, AuNPs/MXene based E-CRISPR retain more than 70% of initial current after 2 months and deliver reliable analytical results that are unaffected over 42-day storage. Owing to its excellent biofouling-resistant and analytical performance and robust shelf life, our E-CRISPR sensor offers a universal, scalable and low-cost strategy for POC nucleic acid testing.
Original languageEnglish
Article number133342
Pages (from-to)1-9
Number of pages9
JournalSensors and Actuators B: Chemical
Volume380
DOIs
Publication statusPublished - 1 Apr 2023

Keywords

  • CRISPR diagnosis
  • Electrochemical biosensor
  • Gold nanoparticles
  • MXene Ti₃C₂
  • Human papillomavirus detection

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