A decade of advances in transposon-insertion sequencing

Amy K. Cain*, Lars Barquist, Andrew L. Goodman, Ian T. Paulsen, Julian Parkhill, Tim van Opijnen

*Corresponding author for this work

Research output: Contribution to journalReview article

Abstract

It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS) methods, which combine genome-wide transposon mutagenesis with high-throughput sequencing to estimate the fitness contribution or essentiality of each genetic component in a bacterial genome. Four TIS variations were published in 2009: transposon sequencing (Tn-Seq), transposon-directed insertion site sequencing (TraDIS), insertion sequencing (INSeq) and high-throughput insertion tracking by deep sequencing (HITS). TIS has since become an important tool for molecular microbiologists, being one of the few genome-wide techniques that directly links phenotype to genotype and ultimately can assign gene function. In this Review, we discuss the recent applications of TIS to answer overarching biological questions. We explore emerging and multidisciplinary methods that build on TIS, with an eye towards future applications.

Original languageEnglish
JournalNature Reviews Genetics
DOIs
Publication statusE-pub ahead of print - 12 Jun 2020

Fingerprint Dive into the research topics of 'A decade of advances in transposon-insertion sequencing'. Together they form a unique fingerprint.

  • Cite this