A decade of advances in transposon-insertion sequencing

Amy K. Cain*, Lars Barquist, Andrew L. Goodman, Ian T. Paulsen, Julian Parkhill, Tim van Opijnen

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    62 Citations (Scopus)
    17 Downloads (Pure)


    It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS) methods, which combine genome-wide transposon mutagenesis with high-throughput sequencing to estimate the fitness contribution or essentiality of each genetic component in a bacterial genome. Four TIS variations were published in 2009: transposon sequencing (Tn-Seq), transposon-directed insertion site sequencing (TraDIS), insertion sequencing (INSeq) and high-throughput insertion tracking by deep sequencing (HITS). TIS has since become an important tool for molecular microbiologists, being one of the few genome-wide techniques that directly links phenotype to genotype and ultimately can assign gene function. In this Review, we discuss the recent applications of TIS to answer overarching biological questions. We explore emerging and multidisciplinary methods that build on TIS, with an eye towards future applications.

    Original languageEnglish
    Pages (from-to)526-540
    Number of pages15
    JournalNature Reviews Genetics
    Issue number9
    Early online date12 Jun 2020
    Publication statusPublished - Sep 2020

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