A fluorescence-based assay for indoleamine 2,3-dioxygenase

Azadeh Matin, Isla M. Streete, Ian M. Jamie, Roger J W Truscott, Joanne F. Jamie*

*Corresponding author for this work

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A rapid and sensitive fluorescence-based bioassay for determination of indoleamine 2,3-dioxygenase (IDO) activity has been developed. This assay relies on the quantification of the amount of kynurenine produced in the assay medium by fluorescence and complements the standard absorbance and high-performance liquid chromatography (HPLC) assay methods. The fluorescence method has limits of detection similar to those of the standard assay methods. Measured activities of IDO, including in the presence of tryptophan-based inhibitors, were in statistical agreement with the absorbance and HPLC assay methods. The fluorescence-based assay was also suitable for assessment of IDO inhibition by compounds that are incompatible with the absorbance method.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalAnalytical Biochemistry
Volume349
Issue number1
DOIs
Publication statusPublished - 1 Feb 2006

Keywords

  • Fluorescence assay
  • Inhibitors
  • Kynurenine
  • Recombinant human indoleamine 2,3-dioxygenase

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