TY - JOUR
T1 - A genetic and structural analysis of the -glycosylation capabilities
AU - Leonard, Renaud
AU - Kolarich, Daniel Konrad
AU - Paschinger, Katharina
AU - Altmann, Friedrich
AU - Wilson, Iain
PY - 2004
Y1 - 2004
N2 - The recent draft sequencing of the rice (Oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. In this study, we have not only identified genes putatively encoding enzymes involved in N-glycosylation, but have examined by MALDI-TOF MS the structures of the N-glycans of rice and other monocotyledons (maize, wheat and dates; Zea mays, Triticum aestivum and Phoenix dactylifera); these data show that within the plant kingdom the types of N-glycans found are very similar between monocotyledons, dicotyledons and gymnosperms. Subsequently, we constructed expression vectors for the key enzymes forming plant-typical structures in rice, N-acetylglucosaminyltransferase I (GlcNAc-TI; EC 2.4.1.101), core α1,3-fucosyltransferase (FucTA; EC 2.4.1.214) and β1,2-xylosyltransferase (EC 2.4.2.38) and successfully expressed them in Pichia pastoris. Rice GlcNAc-TI, FucTA and xylosyltransferase are therefore the first monocotyledon glycosyltransferases involved in N-glycan biosynthesis to be characterised in a recombinant form.
AB - The recent draft sequencing of the rice (Oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. In this study, we have not only identified genes putatively encoding enzymes involved in N-glycosylation, but have examined by MALDI-TOF MS the structures of the N-glycans of rice and other monocotyledons (maize, wheat and dates; Zea mays, Triticum aestivum and Phoenix dactylifera); these data show that within the plant kingdom the types of N-glycans found are very similar between monocotyledons, dicotyledons and gymnosperms. Subsequently, we constructed expression vectors for the key enzymes forming plant-typical structures in rice, N-acetylglucosaminyltransferase I (GlcNAc-TI; EC 2.4.1.101), core α1,3-fucosyltransferase (FucTA; EC 2.4.1.214) and β1,2-xylosyltransferase (EC 2.4.2.38) and successfully expressed them in Pichia pastoris. Rice GlcNAc-TI, FucTA and xylosyltransferase are therefore the first monocotyledon glycosyltransferases involved in N-glycan biosynthesis to be characterised in a recombinant form.
KW - Fucosyltransferase
KW - Glucosyltransferase
KW - Glycan
KW - Monocotyledon
KW - N-acetylglucosaminyltransferase
KW - Oligosaccharide
KW - Rice
KW - Xylosyltransferase
UR - http://www.scopus.com/inward/record.url?scp=16544370449&partnerID=8YFLogxK
U2 - 10.1007/s11103-004-1558-3
DO - 10.1007/s11103-004-1558-3
M3 - Article
C2 - 15604706
AN - SCOPUS:16544370449
SN - 0167-4412
VL - 55
SP - 631
EP - 644
JO - Plant Molecular Biology
JF - Plant Molecular Biology
IS - 5
ER -