A genetic and structural analysis of the -glycosylation capabilities

Renaud Leonard, Daniel Konrad Kolarich, Katharina Paschinger, Friedrich Altmann, Iain Wilson

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    39 Citations (Scopus)

    Abstract

    The recent draft sequencing of the rice (Oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. In this study, we have not only identified genes putatively encoding enzymes involved in N-glycosylation, but have examined by MALDI-TOF MS the structures of the N-glycans of rice and other monocotyledons (maize, wheat and dates; Zea mays, Triticum aestivum and Phoenix dactylifera); these data show that within the plant kingdom the types of N-glycans found are very similar between monocotyledons, dicotyledons and gymnosperms. Subsequently, we constructed expression vectors for the key enzymes forming plant-typical structures in rice, N-acetylglucosaminyltransferase I (GlcNAc-TI; EC 2.4.1.101), core α1,3-fucosyltransferase (FucTA; EC 2.4.1.214) and β1,2-xylosyltransferase (EC 2.4.2.38) and successfully expressed them in Pichia pastoris. Rice GlcNAc-TI, FucTA and xylosyltransferase are therefore the first monocotyledon glycosyltransferases involved in N-glycan biosynthesis to be characterised in a recombinant form.

    Original languageEnglish
    Pages (from-to)631-644
    Number of pages14
    JournalPlant Molecular Biology
    Volume55
    Issue number5
    DOIs
    Publication statusPublished - 2004

    Keywords

    • Fucosyltransferase
    • Glucosyltransferase
    • Glycan
    • Monocotyledon
    • N-acetylglucosaminyltransferase
    • Oligosaccharide
    • Rice
    • Xylosyltransferase

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