A genetic and structural analysis of the -glycosylation capabilities

Renaud Leonard, Daniel Konrad Kolarich, Katharina Paschinger, Friedrich Altmann, Iain Wilson

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The recent draft sequencing of the rice (Oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. In this study, we have not only identified genes putatively encoding enzymes involved in N-glycosylation, but have examined by MALDI-TOF MS the structures of the N-glycans of rice and other monocotyledons (maize, wheat and dates; Zea mays, Triticum aestivum and Phoenix dactylifera); these data show that within the plant kingdom the types of N-glycans found are very similar between monocotyledons, dicotyledons and gymnosperms. Subsequently, we constructed expression vectors for the key enzymes forming plant-typical structures in rice, N-acetylglucosaminyltransferase I (GlcNAc-TI; EC, core α1,3-fucosyltransferase (FucTA; EC and β1,2-xylosyltransferase (EC and successfully expressed them in Pichia pastoris. Rice GlcNAc-TI, FucTA and xylosyltransferase are therefore the first monocotyledon glycosyltransferases involved in N-glycan biosynthesis to be characterised in a recombinant form.

Original languageEnglish
Pages (from-to)631-644
Number of pages14
JournalPlant Molecular Biology
Issue number5
Publication statusPublished - 2004


  • Fucosyltransferase
  • Glucosyltransferase
  • Glycan
  • Monocotyledon
  • N-acetylglucosaminyltransferase
  • Oligosaccharide
  • Rice
  • Xylosyltransferase

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    Leonard, R., Kolarich, D. K., Paschinger, K., Altmann, F., & Wilson, I. (2004). A genetic and structural analysis of the -glycosylation capabilities. Plant Molecular Biology, 55(5), 631-644. https://doi.org/10.1007/s11103-004-1558-3