Abstract
A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in λEMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin α-subunit has been obtained and sequenced. The principal α1 and α2-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 α-subunit genes. The nucleotide sequence of the α-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.
Original language | English |
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Pages (from-to) | 191-194 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 273 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 29 Oct 1990 |
Bibliographical note
Corrigendum can be found at FEBS Letters, 279(2), p. 361, 1991.https://doi.org/10.1016/0014-5793(91)80187-8
Keywords
- Amino acid sequence
- Chroomonas CS24
- Cryptophyceae
- Genomic clone
- Phycoerythrin
- Signal sequence
- α-Subunit