A genomic clone encoding a cryptophyte phycoerythrin α-subunit: Evidence for three α-subunits and an N-terminal membrane transit sequence

Jon Jenkins; Roger G. Hiller; Jim Speirs; Jasminka Godovac-Zimmermann

doi:10.1016/0014-5793(90)81082-Y

A genomic clone encoding a cryptophyte phycoerythrin α-subunit: Evidence for three α-subunits and an N-terminal membrane transit sequence

Jon Jenkins, Roger G. Hiller^*, Jim Speirs, Jasminka Godovac-Zimmermann

^*Corresponding author for this work

Faculty of Science and Engineering

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in λEMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin α-subunit has been obtained and sequenced. The principal α₁ and α₂-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 α-subunit genes. The nucleotide sequence of the α-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.

Original language	English
Pages (from-to)	191-194
Number of pages	4
Journal	FEBS Letters
Volume	273
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(90)81082-Y
Publication status	Published - 29 Oct 1990

Bibliographical note

Corrigendum can be found at FEBS Letters, 279(2), p. 361, 1991.
https://doi.org/10.1016/0014-5793(91)80187-8

Keywords

Amino acid sequence
Chroomonas CS24
Cryptophyceae
Genomic clone
Phycoerythrin
Signal sequence
α-Subunit

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10.1016/0014-5793(90)81082-Y

Cite this

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title = "A genomic clone encoding a cryptophyte phycoerythrin α-subunit: Evidence for three α-subunits and an N-terminal membrane transit sequence ",

abstract = "A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in λEMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin α-subunit has been obtained and sequenced. The principal α1 and α2-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 α-subunit genes. The nucleotide sequence of the α-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.",

keywords = "Amino acid sequence, Chroomonas CS24, Cryptophyceae, Genomic clone, Phycoerythrin, Signal sequence, α-Subunit",

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note = "Corrigendum can be found at FEBS Letters, 279(2), p. 361, 1991. https://doi.org/10.1016/0014-5793(91)80187-8",

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AU - Hiller, Roger G.

AU - Speirs, Jim

AU - Godovac-Zimmermann, Jasminka

N1 - Corrigendum can be found at FEBS Letters, 279(2), p. 361, 1991. https://doi.org/10.1016/0014-5793(91)80187-8

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N2 - A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in λEMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin α-subunit has been obtained and sequenced. The principal α1 and α2-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 α-subunit genes. The nucleotide sequence of the α-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.

AB - A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in λEMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin α-subunit has been obtained and sequenced. The principal α1 and α2-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 α-subunit genes. The nucleotide sequence of the α-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.

KW - Amino acid sequence

KW - Chroomonas CS24

KW - Cryptophyceae

KW - Genomic clone

KW - Phycoerythrin

KW - Signal sequence

KW - α-Subunit

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A genomic clone encoding a cryptophyte phycoerythrin α-subunit: Evidence for three α-subunits and an N-terminal membrane transit sequence

Abstract

Bibliographical note

Keywords

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