A genomic clone encoding a cryptophyte phycoerythrin α-subunit: Evidence for three α-subunits and an N-terminal membrane transit sequence

Jon Jenkins, Roger G. Hiller*, Jim Speirs, Jasminka Godovac-Zimmermann

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in λEMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin α-subunit has been obtained and sequenced. The principal α1 and α2-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 α-subunit genes. The nucleotide sequence of the α-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalFEBS Letters
Volume273
Issue number1-2
DOIs
Publication statusPublished - 29 Oct 1990

Bibliographical note

Corrigendum can be found at FEBS Letters, 279(2), p. 361, 1991.
https://doi.org/10.1016/0014-5793(91)80187-8

Keywords

  • Amino acid sequence
  • Chroomonas CS24
  • Cryptophyceae
  • Genomic clone
  • Phycoerythrin
  • Signal sequence
  • α-Subunit

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