A gloverin-like antibacterial protein is synthesized in Helicoverpa armigera following bacterial challenge

James A. Mackintosh, Andrew A. Gooley, Peter H. Karuso, Andrew J. Beattie, Daniel R. Jardine, Duncan A. Veal

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A bacteria inducible antibacterial protein, P2, was isolated from the old world boll-worm Helicoverpa armigera. Fifth-instar larvae were injected with live Escherichia coli NCTC 8196. P2 was isolated by HPLC using reversed- phase and size-exclusion columns. In addition, P2 was isolated by an alternative method of sequential cation-exchange and reversed-phase HPLC. The structure of P2 was determined by N-terminal Edman degradation and mass spectrometry. P2 had similar mass (14.1 kDa) structure and activity to gloverin, an inducible glycine-rich antibacterial protein isolated from Hyalophora gloveri [Axen, A.; Carlsson, A.; Engstrom, Å.; Bennich, H. Eur. J. Biochem. 247:614-619; 1997]. At the N-terminus P2 had ~60% identity with gloverin. P2 is basic, heat stable, and displayed rapid antibacterial action. P2 was active against the Gram-negative bacteria tested and was inactive against the Gram-positive bacteria, Candida albicans, a bovine turbinate cell line, and pestivirus.

LanguageEnglish
Pages387-399
Number of pages13
JournalDevelopmental and Comparative Immunology
Volume22
Issue number4
DOIs
Publication statusPublished - 1 Jul 1998

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Pestivirus
High Pressure Liquid Chromatography
Turbinates
Gram-Positive Bacteria
Gram-Negative Bacteria
Candida albicans
Glycine
Larva
Cations
Mass Spectrometry
Proteins
Hot Temperature
Escherichia coli
Bacteria
Cell Line
gloverin

Keywords

  • gloverin
  • helicoverpa armigera
  • insect immunity
  • peptide antibiotics

Cite this

Mackintosh, James A. ; Gooley, Andrew A. ; Karuso, Peter H. ; Beattie, Andrew J. ; Jardine, Daniel R. ; Veal, Duncan A. / A gloverin-like antibacterial protein is synthesized in Helicoverpa armigera following bacterial challenge. In: Developmental and Comparative Immunology. 1998 ; Vol. 22, No. 4. pp. 387-399.
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A gloverin-like antibacterial protein is synthesized in Helicoverpa armigera following bacterial challenge. / Mackintosh, James A.; Gooley, Andrew A.; Karuso, Peter H.; Beattie, Andrew J.; Jardine, Daniel R.; Veal, Duncan A.

In: Developmental and Comparative Immunology, Vol. 22, No. 4, 01.07.1998, p. 387-399.

Research output: Contribution to journalArticleResearchpeer-review

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