TY - JOUR
T1 - A gloverin-like antibacterial protein is synthesized in Helicoverpa armigera following bacterial challenge
AU - Mackintosh, James A.
AU - Gooley, Andrew A.
AU - Karuso, Peter H.
AU - Beattie, Andrew J.
AU - Jardine, Daniel R.
AU - Veal, Duncan A.
PY - 1998/7/1
Y1 - 1998/7/1
N2 - A bacteria inducible antibacterial protein, P2, was isolated from the old world boll-worm Helicoverpa armigera. Fifth-instar larvae were injected with live Escherichia coli NCTC 8196. P2 was isolated by HPLC using reversed- phase and size-exclusion columns. In addition, P2 was isolated by an alternative method of sequential cation-exchange and reversed-phase HPLC. The structure of P2 was determined by N-terminal Edman degradation and mass spectrometry. P2 had similar mass (14.1 kDa) structure and activity to gloverin, an inducible glycine-rich antibacterial protein isolated from Hyalophora gloveri [Axen, A.; Carlsson, A.; Engstrom, Å.; Bennich, H. Eur. J. Biochem. 247:614-619; 1997]. At the N-terminus P2 had ~60% identity with gloverin. P2 is basic, heat stable, and displayed rapid antibacterial action. P2 was active against the Gram-negative bacteria tested and was inactive against the Gram-positive bacteria, Candida albicans, a bovine turbinate cell line, and pestivirus.
AB - A bacteria inducible antibacterial protein, P2, was isolated from the old world boll-worm Helicoverpa armigera. Fifth-instar larvae were injected with live Escherichia coli NCTC 8196. P2 was isolated by HPLC using reversed- phase and size-exclusion columns. In addition, P2 was isolated by an alternative method of sequential cation-exchange and reversed-phase HPLC. The structure of P2 was determined by N-terminal Edman degradation and mass spectrometry. P2 had similar mass (14.1 kDa) structure and activity to gloverin, an inducible glycine-rich antibacterial protein isolated from Hyalophora gloveri [Axen, A.; Carlsson, A.; Engstrom, Å.; Bennich, H. Eur. J. Biochem. 247:614-619; 1997]. At the N-terminus P2 had ~60% identity with gloverin. P2 is basic, heat stable, and displayed rapid antibacterial action. P2 was active against the Gram-negative bacteria tested and was inactive against the Gram-positive bacteria, Candida albicans, a bovine turbinate cell line, and pestivirus.
KW - gloverin
KW - helicoverpa armigera
KW - insect immunity
KW - peptide antibiotics
UR - http://www.scopus.com/inward/record.url?scp=0031846514&partnerID=8YFLogxK
U2 - 10.1016/S0145-305X(98)00025-1
DO - 10.1016/S0145-305X(98)00025-1
M3 - Article
C2 - 9699484
AN - SCOPUS:0031846514
SN - 0145-305X
VL - 22
SP - 387
EP - 399
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 4
ER -