A hemocyte classification scheme for the tunicate Styela plicata

Jane L. Radford, Aimee E. Hutchinson, Monika Burandt, David A. Raftos*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)


    Many classification systems have been developed to identify different types of tunicate hemocytes. However, no one system has used a multi- parameter approach to classify hemocytes from the solitary tunicate Styela plicata. This study provides such a multi-parameter classification scheme for S. plicata hemocytes. Three broad classes of hemocyte encompassing eight individual hemocyte types were identified. Classifications were based on bright and dark field microscopy, as well as immunofluorescence staining and immunohistochemistry using a suite of anti-hemocyte monoclonal antibodies. Flow cytometry was also used to complement the classification system as a rapid method to identify S. plicata hemocyte sub-populations.

    Original languageEnglish
    Pages (from-to)343-350
    Number of pages8
    JournalActa Zoologica
    Issue number4
    Publication statusPublished - Oct 1998


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