A hemocyte classification scheme for the tunicate Styela plicata

Jane L. Radford, Aimee E. Hutchinson, Monika Burandt, David A. Raftos*

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Many classification systems have been developed to identify different types of tunicate hemocytes. However, no one system has used a multi- parameter approach to classify hemocytes from the solitary tunicate Styela plicata. This study provides such a multi-parameter classification scheme for S. plicata hemocytes. Three broad classes of hemocyte encompassing eight individual hemocyte types were identified. Classifications were based on bright and dark field microscopy, as well as immunofluorescence staining and immunohistochemistry using a suite of anti-hemocyte monoclonal antibodies. Flow cytometry was also used to complement the classification system as a rapid method to identify S. plicata hemocyte sub-populations.

Original languageEnglish
Pages (from-to)343-350
Number of pages8
JournalActa Zoologica
Volume79
Issue number4
DOIs
Publication statusPublished - Oct 1998

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