@inbook{577d660544464ef692c95fd81489ed40,
title = "A high-throughput colorimetric microplate assay for determination of plasma arginase activity",
abstract = "Arginase, an enzyme involved in the urea cycle, is gaining attention as a critical player in numerous chronic pathologies. Additionally, increased activity of this enzyme has been shown to correlate with poor prognosis in a range of cancers. Colorimetric assays that measure the conversion of arginine to ornithine have long been used to determine the activity of arginase. However, this analysis is hindered by a lack of standardization across protocols. Here, we describe in detail a novel revision of the Chinard{\textquoteright}s colorimetric assay used to determine arginase activity. Dilution series of patient plasma are plotted to form a logistic function, from which activity can be interpolated by comparison to an ornithine standard curve. Inclusion of patient dilution series rather than a single point increases the robustness of the assay. This high-throughput microplate assay analyzes 10 samples per plate to produce highly reproducible results.",
keywords = "Arginase, Arginine, Colorimetric assay, Enzyme activity, Microplate",
author = "Smith, {Natalie J.} and Mahnaz Maddahfar and Bavani Gunasegaran and McGuire, {Helen M.} and {Fazekas de St Groth}, Barbara",
year = "2023",
month = apr,
day = "18",
doi = "10.1007/978-1-0716-2942-0_29",
language = "English",
isbn = "9781071629413",
series = "Methods in Molecular Biology",
publisher = "The Humana Press",
pages = "273--286",
editor = "Kashina, {Anna S.}",
booktitle = "Protein arginylation",
edition = "2",
}