A humoral opsonin from the solitary urochordate Styela clava

Karen L. Kelly*, Edwin L. Cooper, David A. Raftos

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Opsonins play a key role in invertebrate humoral immune systems. An opsonin for yeast was identified in the plasma of the tunicate, Styela clava. In vitro cultures of hemocytes with homologous plasma-incubated yeast exhibited significantly higher levels of phagocytosis than controls. Studies indicated that the opsonic activity of Styela clava plasma increased the overall capacity for phagocytosis. Opsonization was inhibited by the carbohydrates mannan, N-acetyl-d-galactosamine, and galactose, and by the divalent cation chelator, EDTA. These data suggest that the Styela clava opsonin may share some functional similarities with a C-type lectin. Incubation of yeast with Styela clava and Styela plicata plasma prior to phagocytosis by hemocytes from both species indicated the Styela clava opsonin is species specific.

Original languageEnglish
Pages (from-to)29-39
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume17
Issue number1
DOIs
Publication statusPublished - 1993
Externally publishedYes

Keywords

  • Humoral immunity
  • Lectin
  • Opsonin
  • Tunicate
  • Urochordate

Fingerprint Dive into the research topics of 'A humoral opsonin from the solitary urochordate Styela clava'. Together they form a unique fingerprint.

  • Cite this