A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome

Brenda D. Wingfield; Valerie J. Southgate; Isak S. Pretorius; Hendrik J J Van Vuuren

doi:10.1002/yea.320060210

A K₂ neutral Saccharomyces cerevisiae strain contains a variant K₂ M genome

Brenda D. Wingfield^*, Valerie J. Southgate, Isak S. Pretorius, Hendrik J J Van Vuuren

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

K₂ neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double‐stranded RNA (dsRNA) genome encoding for resistance to K₂ toxin. The M dsRNA was larger than the K₂ killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K₂ neutral M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K₂ M genome had homology with K₂ neutral M genome. Hybridization showed that the USM12 M dsRNA has significant homology with the K₂M dsRNA. Protein profiles of extracellular proteins from USMA12 and cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K₂ neutral yeast strain has been characterized.

Original language	English
Pages (from-to)	159-169
Number of pages	11
Journal	Yeast
Volume	6
Issue number	2
DOIs	https://doi.org/10.1002/yea.320060210
Publication status	Published - 1990
Externally published	Yes

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title = "A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome",

abstract = "K2 neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double‐stranded RNA (dsRNA) genome encoding for resistance to K2 toxin. The M dsRNA was larger than the K2 killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K2 neutral M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K2 M genome had homology with K2 neutral M genome. Hybridization showed that the USM12 M dsRNA has significant homology with the K2M dsRNA. Protein profiles of extracellular proteins from USMA12 and cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K2 neutral yeast strain has been characterized.",

author = "Wingfield, {Brenda D.} and Southgate, {Valerie J.} and Pretorius, {Isak S.} and {Van Vuuren}, {Hendrik J J}",

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T1 - A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome

AU - Wingfield, Brenda D.

AU - Southgate, Valerie J.

AU - Pretorius, Isak S.

AU - Van Vuuren, Hendrik J J

PY - 1990

Y1 - 1990

N2 - K2 neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double‐stranded RNA (dsRNA) genome encoding for resistance to K2 toxin. The M dsRNA was larger than the K2 killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K2 neutral M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K2 M genome had homology with K2 neutral M genome. Hybridization showed that the USM12 M dsRNA has significant homology with the K2M dsRNA. Protein profiles of extracellular proteins from USMA12 and cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K2 neutral yeast strain has been characterized.

AB - K2 neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double‐stranded RNA (dsRNA) genome encoding for resistance to K2 toxin. The M dsRNA was larger than the K2 killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K2 neutral M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K2 M genome had homology with K2 neutral M genome. Hybridization showed that the USM12 M dsRNA has significant homology with the K2M dsRNA. Protein profiles of extracellular proteins from USMA12 and cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K2 neutral yeast strain has been characterized.

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DO - 10.1002/yea.320060210

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VL - 6

SP - 159

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JO - Yeast

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ER -

A K₂ neutral Saccharomyces cerevisiae strain contains a variant K₂ M genome

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