A method for high-throughput image-based antifungal screening

Rosalie Sabburg; Aphrika Gregson; Andrew S. Urquhart; Elizabeth A. B. Aitken; Linda Smith; Louise F. Thatcher; Donald M. Gardiner

doi:10.1016/j.mimet.2021.106342

A method for high-throughput image-based antifungal screening

Rosalie Sabburg, Aphrika Gregson, Andrew S. Urquhart, Elizabeth A. B. Aitken, Linda Smith, Louise F. Thatcher, Donald M. Gardiner

Applied BioSciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Robust antifungal screening is technically challenging particularly for filamentous fungi. We present a method for undertaking antifungal screening assays that builds upon existing broth dilution protocols and incorporates time resolved image-based assessment of fungal growth. We show that the method performs with different fungi, particularly those for which spores can be used as inoculum, and with different compound classes, can accurately assess susceptibility or otherwise in only few hours and can even account for differences in inherent growth properties of strains.

Original language	English
Article number	106342
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of Microbiological Methods
Volume	190
Early online date	5 Oct 2021
DOIs	https://doi.org/10.1016/j.mimet.2021.106342
Publication status	Published - Nov 2021

Keywords

Antifungal
Verticillium
Aspergillus
Fusarium
Rapid
Susceptibility

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10.1016/j.mimet.2021.106342

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keywords = "Antifungal, Verticillium, Aspergillus, Fusarium, Rapid, Susceptibility",

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AU - Sabburg, Rosalie

AU - Gregson, Aphrika

AU - Urquhart, Andrew S.

AU - Aitken, Elizabeth A. B.

AU - Smith, Linda

AU - Thatcher, Louise F.

AU - Gardiner, Donald M.

PY - 2021/11

Y1 - 2021/11

N2 - Robust antifungal screening is technically challenging particularly for filamentous fungi. We present a method for undertaking antifungal screening assays that builds upon existing broth dilution protocols and incorporates time resolved image-based assessment of fungal growth. We show that the method performs with different fungi, particularly those for which spores can be used as inoculum, and with different compound classes, can accurately assess susceptibility or otherwise in only few hours and can even account for differences in inherent growth properties of strains.

AB - Robust antifungal screening is technically challenging particularly for filamentous fungi. We present a method for undertaking antifungal screening assays that builds upon existing broth dilution protocols and incorporates time resolved image-based assessment of fungal growth. We show that the method performs with different fungi, particularly those for which spores can be used as inoculum, and with different compound classes, can accurately assess susceptibility or otherwise in only few hours and can even account for differences in inherent growth properties of strains.

KW - Antifungal

KW - Verticillium

KW - Aspergillus

KW - Fusarium

KW - Rapid

KW - Susceptibility

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JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

M1 - 106342

ER -

A method for high-throughput image-based antifungal screening

Abstract

Keywords

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