A method for high-throughput image-based antifungal screening

Rosalie Sabburg, Aphrika Gregson, Andrew S. Urquhart, Elizabeth A. B. Aitken, Linda Smith, Louise F. Thatcher, Donald M. Gardiner

Research output: Contribution to journalArticlepeer-review


Robust antifungal screening is technically challenging particularly for filamentous fungi. We present a method for undertaking antifungal screening assays that builds upon existing broth dilution protocols and incorporates time resolved image-based assessment of fungal growth. We show that the method performs with different fungi, particularly those for which spores can be used as inoculum, and with different compound classes, can accurately assess susceptibility or otherwise in only few hours and can even account for differences in inherent growth properties of strains.
Original languageEnglish
Article number106342
Pages (from-to)1-7
Number of pages7
JournalJournal of Microbiological Methods
Early online date5 Oct 2021
Publication statusPublished - Nov 2021


  • Antifungal
  • Verticillium
  • Aspergillus
  • Fusarium
  • Rapid
  • Susceptibility


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