A Method for in vivo quantification of cytokine IL-1β in the rat intrathecal space

Fei Deng; Azim Arman; Ewa M. Goldys; Mark R. Hutchinson; Guozhen Liu

doi:10.1021/acsabm.9b00958

A Method for in vivo quantification of cytokine IL-1β in the rat intrathecal space

Fei Deng, Azim Arman, Ewa M. Goldys, Mark R. Hutchinson, Guozhen Liu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL^-1) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague-Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.

Original language	English
Pages (from-to)	539-546
Number of pages	8
Journal	ACS Applied Bio Materials
Volume	3
Issue number	1
DOIs	https://doi.org/10.1021/acsabm.9b00958
Publication status	Published - 21 Jan 2020
Externally published	Yes

Keywords

cytokine sensing
IL-1β
longitudinal experiments
rat intrathecal space
spinal cord

Access to Document

10.1021/acsabm.9b00958

Cite this

@article{960b45c55ce74c1fb8c0c4e6463d32ae,

title = "A Method for in vivo quantification of cytokine IL-1β in the rat intrathecal space",

abstract = "IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL-1) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague-Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.",

keywords = "cytokine sensing, IL-1β, longitudinal experiments, rat intrathecal space, spinal cord",

author = "Fei Deng and Azim Arman and Goldys, {Ewa M.} and Hutchinson, {Mark R.} and Guozhen Liu",

year = "2020",

month = jan,

day = "21",

doi = "10.1021/acsabm.9b00958",

language = "English",

volume = "3",

pages = "539--546",

journal = "ACS Applied Bio Materials",

issn = "2576-6422",

publisher = "American Chemical Society",

number = "1",

}

TY - JOUR

T1 - A Method for in vivo quantification of cytokine IL-1β in the rat intrathecal space

AU - Deng, Fei

AU - Arman, Azim

AU - Goldys, Ewa M.

AU - Hutchinson, Mark R.

AU - Liu, Guozhen

PY - 2020/1/21

Y1 - 2020/1/21

N2 - IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL-1) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague-Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.

AB - IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL-1) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague-Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.

KW - cytokine sensing

KW - IL-1β

KW - longitudinal experiments

KW - rat intrathecal space

KW - spinal cord

UR - http://www.scopus.com/inward/record.url?scp=85077677984&partnerID=8YFLogxK

U2 - 10.1021/acsabm.9b00958

DO - 10.1021/acsabm.9b00958

M3 - Article

C2 - 35019397

AN - SCOPUS:85077677984

SN - 2576-6422

VL - 3

SP - 539

EP - 546

JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

IS - 1

ER -

A Method for in vivo quantification of cytokine IL-1β in the rat intrathecal space

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this