A Method for in vivo quantification of cytokine IL-1β in the rat intrathecal space

Fei Deng, Azim Arman, Ewa M. Goldys, Mark R. Hutchinson, Guozhen Liu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL-1) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague-Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.

Original languageEnglish
Pages (from-to)539-546
Number of pages8
JournalACS Applied Bio Materials
Volume3
Issue number1
DOIs
Publication statusPublished - 21 Jan 2020
Externally publishedYes

Keywords

  • cytokine sensing
  • IL-1β
  • longitudinal experiments
  • rat intrathecal space
  • spinal cord

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