A method for the detection of IgE binding sequences of allergens based on a modification of epitope mapping

B. J. Walsh, M. E H Howden*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

An ELISA method for the rapid determination of IgE binding sites (allergenic determinants) of proteins is reported. The method utilizes the epitope mapping kit (Geysen et al., 1984) to synthesize hexapeptides of an allergen of interest, followed by a biotin-avidin system to detect peptide-bound IgE. The technique allows rapid localisation of determinants from allergens of known sequence without the need to purify large amounts of allergen nor to generate peptides by cleavage of it. Using the results of the epitope mapping experiments a putative allergenic peptide containing 18 amino acid residues from the sequence of a wheat allergen was identified and synthesised on polyamide resin. Testing of this peptide by radioallergosorbent test (RAST) inhibition showed that it bound specific IgE in the sera of patients allergic to wheat.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalJournal of Immunological Methods
Volume121
Issue number2
DOIs
Publication statusPublished - 26 Jul 1989

Keywords

  • Allergen-specific IgE
  • Avidin-biotin system
  • Epitope mapping

Fingerprint

Dive into the research topics of 'A method for the detection of IgE binding sequences of allergens based on a modification of epitope mapping'. Together they form a unique fingerprint.

Cite this