A method for the detection of IgE binding sequences of allergens based on a modification of epitope mapping

B. J. Walsh, M. E H Howden*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    59 Citations (Scopus)

    Abstract

    An ELISA method for the rapid determination of IgE binding sites (allergenic determinants) of proteins is reported. The method utilizes the epitope mapping kit (Geysen et al., 1984) to synthesize hexapeptides of an allergen of interest, followed by a biotin-avidin system to detect peptide-bound IgE. The technique allows rapid localisation of determinants from allergens of known sequence without the need to purify large amounts of allergen nor to generate peptides by cleavage of it. Using the results of the epitope mapping experiments a putative allergenic peptide containing 18 amino acid residues from the sequence of a wheat allergen was identified and synthesised on polyamide resin. Testing of this peptide by radioallergosorbent test (RAST) inhibition showed that it bound specific IgE in the sera of patients allergic to wheat.

    Original languageEnglish
    Pages (from-to)275-280
    Number of pages6
    JournalJournal of Immunological Methods
    Volume121
    Issue number2
    DOIs
    Publication statusPublished - 26 Jul 1989

    Keywords

    • Allergen-specific IgE
    • Avidin-biotin system
    • Epitope mapping

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