A method for topical dosing of invertebrates with pesticide for use in feeding experiments

Paul Story, Kristine French, Kimberly Maute, Andrew C. Warden, Greg Dojchinov, Grant C. Hose*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to produce large numbers of pesticide-exposed insects (e.g. crickets) is important for feeding studies into the effects of pesticides on key predatory species. House crickets (Acheta domesticus L. 1758) were submersed in serial dilutions of the pesticides, fenitrothion and fipronil, used for the control of locusts in Australia, and then rapidly frozen for residue analysis. Good correlations were found between increasing concentrations of serial pesticide dilutions and the resultant residual concentrations of the parent compounds in crickets, with R2 values of 0.949 (fenitrothion) and 0.946 (fipronil). R2 values for the much less abundant fipronil metabolites were lower 0.858 (sulfone), 0.368 (desulfinyl) and 0.785 (sulfide). This method enables insecticide exposure mimicking the field conditions to be assessed, and can be done immediately prior to an experiment. This ensures locusts remain alive when introduced to the feeding chambers, and enables multiple prey items to be dosed with a known pesticide burden.

Original languageEnglish
Pages (from-to)381-386
Number of pages6
JournalEcotoxicology
Volume30
Issue number2
Early online date14 Jan 2021
DOIs
Publication statusPublished - Mar 2021

Keywords

  • Feeding studies
  • Fenitrothion
  • Fipronil
  • Invertebrate residues
  • Spiking method

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