A microwave technique for double indirect immunostaining of human brain tissue cultures with mouse monoclonal antibodies

Nicholas E. Jufas, Ben Roediger, Patricia J. Armati*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The use of 2 monoclonal antibodies during double immunohistochemistry would enable the use of a greater variety of antibody combinations. Here, we demonstrate a simple, cost effective method of double indirect immunostaining of cultured cells using primary antibodies from the same species. This method uses microwaving of cell samples immediately after the application of the first secondary antibody, and significantly reduces the level of nonspecific staining. This technique does not elute the antibodies, nor raise the sample temperature above 37 degrees C.

Original languageEnglish
Pages (from-to)83-86
Number of pages4
JournalApplied immunohistochemistry & molecular morphology
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 2008
Externally publishedYes

Keywords

  • microwaves
  • cell culture
  • immunohistochemistry
  • PEROXIDASE
  • REACTIVITY
  • ANTIGENS

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