A modular 3D printed microfluidic system: a potential solution for continuous cell harvesting in large-scale bioprocessing

Lin Ding; Sajad Razavi Bazaz; Mahsa Asadniaye Fardjahromi; Flyn McKinnirey; Brian Saputro; Balarka Banerjee; Graham Vesey; Majid Ebrahimi Warkiani

doi:10.1186/s40643-022-00550-2

A modular 3D printed microfluidic system: a potential solution for continuous cell harvesting in large-scale bioprocessing

Lin Ding, Sajad Razavi Bazaz, Mahsa Asadniaye Fardjahromi, Flyn McKinnirey, Brian Saputro, Balarka Banerjee, Graham Vesey, Majid Ebrahimi Warkiani^*

^*Corresponding author for this work

School of Engineering

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

25 Downloads (Pure)

Abstract

Microfluidic devices have shown promising applications in the bioprocessing industry. However, the lack of modularity and high cost of testing and error limit their implementation in the industry. Advances in 3D printing technologies have facilitated the conversion of microfluidic devices from research output to applicable industrial systems. Here, for the first time, we presented a 3D printed modular microfluidic system consisting of two micromixers, one spiral microfluidic separator, and one microfluidic concentrator. We showed that this system can detach and separate mesenchymal stem cells (MSCs) from microcarriers (MCs) in a short time while maintaining the cell’s viability and functionality. The system can be multiplexed and scaled up to process large volumes of the industry. Importantly, this system is a closed system with no human intervention and is promising for current good manufacturing practices.

[Graphical abstract presents]

Original language	English
Article number	64
Pages (from-to)	1-16
Number of pages	16
Journal	Bioresources and Bioprocessing
Volume	9
DOIs	https://doi.org/10.1186/s40643-022-00550-2
Publication status	Published - 2022

Bibliographical note

Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Microfluidics
Modular microfluidic system
Mesenchymal stem cells
3D printing
Bioprocessing

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10.1186/s40643-022-00550-2Licence: CC BY

Publisher version (open access)Final published version, 3.6 MBLicence: CC BY

Cite this

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abstract = "Microfluidic devices have shown promising applications in the bioprocessing industry. However, the lack of modularity and high cost of testing and error limit their implementation in the industry. Advances in 3D printing technologies have facilitated the conversion of microfluidic devices from research output to applicable industrial systems. Here, for the first time, we presented a 3D printed modular microfluidic system consisting of two micromixers, one spiral microfluidic separator, and one microfluidic concentrator. We showed that this system can detach and separate mesenchymal stem cells (MSCs) from microcarriers (MCs) in a short time while maintaining the cell{\textquoteright}s viability and functionality. The system can be multiplexed and scaled up to process large volumes of the industry. Importantly, this system is a closed system with no human intervention and is promising for current good manufacturing practices. [Graphical abstract presents]",

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AU - Razavi Bazaz, Sajad

AU - Asadniaye Fardjahromi, Mahsa

AU - McKinnirey, Flyn

AU - Saputro, Brian

AU - Banerjee, Balarka

AU - Vesey, Graham

AU - Ebrahimi Warkiani, Majid

N1 - Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2022

Y1 - 2022

N2 - Microfluidic devices have shown promising applications in the bioprocessing industry. However, the lack of modularity and high cost of testing and error limit their implementation in the industry. Advances in 3D printing technologies have facilitated the conversion of microfluidic devices from research output to applicable industrial systems. Here, for the first time, we presented a 3D printed modular microfluidic system consisting of two micromixers, one spiral microfluidic separator, and one microfluidic concentrator. We showed that this system can detach and separate mesenchymal stem cells (MSCs) from microcarriers (MCs) in a short time while maintaining the cell’s viability and functionality. The system can be multiplexed and scaled up to process large volumes of the industry. Importantly, this system is a closed system with no human intervention and is promising for current good manufacturing practices. [Graphical abstract presents]

AB - Microfluidic devices have shown promising applications in the bioprocessing industry. However, the lack of modularity and high cost of testing and error limit their implementation in the industry. Advances in 3D printing technologies have facilitated the conversion of microfluidic devices from research output to applicable industrial systems. Here, for the first time, we presented a 3D printed modular microfluidic system consisting of two micromixers, one spiral microfluidic separator, and one microfluidic concentrator. We showed that this system can detach and separate mesenchymal stem cells (MSCs) from microcarriers (MCs) in a short time while maintaining the cell’s viability and functionality. The system can be multiplexed and scaled up to process large volumes of the industry. Importantly, this system is a closed system with no human intervention and is promising for current good manufacturing practices. [Graphical abstract presents]

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A modular 3D printed microfluidic system: a potential solution for continuous cell harvesting in large-scale bioprocessing

Abstract

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