A monoclonal antibody suitable for the radioimmunoassay of prolactin in human serum

Margaret C. Stuart*, P. Anne Underwood, Lynette Boscato

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Monoclonal antibodies directed toward human PRL (hPRL) have been produced by fusion of mouse myeloma cells (Sp2/0-Ag 14) with spleen cells from mice immunized with hPRL. Total immunizing doses of 20 μg and 64 μg hPRL resulted in the production of three highly specific hPRL antibodies. The high affinity antibody, with a Ka value of 0.23 Times 1010 M-1, was used to establish a RIA highly suitable for the measurement of hPRL levels in human serum. The correlation of serum hPRL levels measured using the antibody and those in a conventional rabbit anti-hPRL assay was 0.99 (y = 1.16x - 7.2). These results demonstrate that using the mouse hybridoma technique, it is possible to produce high affinity monospecific monoclonal antibody suitable for the measurement of hPRL in human serum.

Original languageEnglish
Pages (from-to)881-884
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume54
Issue number5
DOIs
Publication statusPublished - 1982

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