Abstract
We have designed a novel nanofluidic device enabling biologics purity and quality test for biologics in less than 1 hour, using a tiny volume of sample. The device developed here has periodically patterned slanted nanofilter arrays, which are utilized not only separate proteins by size but also concentrate them to achieve higher detection sensitivity. Using the device, we demonstrated purity test using simulated impurities (detecting and quantifying aggregation and fragment), as well as quality test using homogeneous peptide binding assay (quantifying proper folding and binding activity). With further development, this nanofluidic device could be used for point-of-care biologics quality assurance in pharmaceutical industry.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 |
| Subtitle of host publication | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 329-331 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 |
| Externally published | Yes |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 |
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country/Territory | Korea, Republic of |
| City | Gyeongju |
| Period | 25/10/15 → 29/10/15 |
Keywords
- Biologics
- Nanofilter
- Purity and quality test
- Size-base separation and concentration