A nanofluidic device for rapid biologics quality control

Sung Hee Ko, Wei Ouyang, Divya Chandra, Annie Yu Wang, Pankaj Karande, William Hancock, Jongyoon Han*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

1 Citation (Scopus)

Abstract

We have designed a novel nanofluidic device enabling biologics purity and quality test for biologics in less than 1 hour, using a tiny volume of sample. The device developed here has periodically patterned slanted nanofilter arrays, which are utilized not only separate proteins by size but also concentrate them to achieve higher detection sensitivity. Using the device, we demonstrated purity test using simulated impurities (detecting and quantifying aggregation and fragment), as well as quality test using homogeneous peptide binding assay (quantifying proper folding and binding activity). With further development, this nanofluidic device could be used for point-of-care biologics quality assurance in pharmaceutical industry.

Original languageEnglish
Title of host publicationMicroTAS 2015
Subtitle of host publication19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages329-331
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Externally publishedYes
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period25/10/1529/10/15

Keywords

  • Biologics
  • Nanofilter
  • Purity and quality test
  • Size-base separation and concentration

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