TY - JOUR
T1 - A new ensemble approach based chemosensor for the reversible detection of bio-thiols and its application in live cell imaging
AU - Wang, Yue
AU - Zhang, Zhiqiang
AU - Meng, Qingtao
AU - He, Cheng
AU - Zhang, Run
AU - Duan, Chunying
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Based on an aldazine-copper chemosensing ensemble (NP-Cu2+), a new fluorescence chemosensor for the detection of biothiols (Cys, Hcy and GSH) was designed and synthesized. In aqueous solution, the ligand NP exhibited high selectivity toward Cu2+ ions by forming a 2:1 complex, accompanied with a dramatic fluorescence quenching and a notable bathochromic-shift of the absorbance band. Due to the high affinity of thiols and copper, the specific interaction of thiols (Cys, Hcy and GSH) with NP-Cu2+ ensemble led to the liberation of the NP. As the result, recovery of fluorescence and UV-vis absorbance was observed. The detection limits of NP-Cu2+ to Cys, Hcy and GSH were estimated to be 1.5 μM, 1.8 μM and 2.2 μM, respectively. The fluorescence "OFF-ON" circle can be repeated to a minimum of 5 times by the alternative addition of thiols and Cu2+, implying that NP-Cu2+ is a recyclable chemosensor for thiols. Results of fluorescence microscopy imaging suggested that NP-Cu2+ has potential to be used as a powerful tool for the detection of intracellular thiols.
AB - Based on an aldazine-copper chemosensing ensemble (NP-Cu2+), a new fluorescence chemosensor for the detection of biothiols (Cys, Hcy and GSH) was designed and synthesized. In aqueous solution, the ligand NP exhibited high selectivity toward Cu2+ ions by forming a 2:1 complex, accompanied with a dramatic fluorescence quenching and a notable bathochromic-shift of the absorbance band. Due to the high affinity of thiols and copper, the specific interaction of thiols (Cys, Hcy and GSH) with NP-Cu2+ ensemble led to the liberation of the NP. As the result, recovery of fluorescence and UV-vis absorbance was observed. The detection limits of NP-Cu2+ to Cys, Hcy and GSH were estimated to be 1.5 μM, 1.8 μM and 2.2 μM, respectively. The fluorescence "OFF-ON" circle can be repeated to a minimum of 5 times by the alternative addition of thiols and Cu2+, implying that NP-Cu2+ is a recyclable chemosensor for thiols. Results of fluorescence microscopy imaging suggested that NP-Cu2+ has potential to be used as a powerful tool for the detection of intracellular thiols.
KW - Chemosensing ensemble
KW - Detection
KW - Fluorescence imaging
KW - NBD
KW - Thiols
UR - http://www.scopus.com/inward/record.url?scp=84960110654&partnerID=8YFLogxK
U2 - 10.1016/j.jlumin.2016.02.032
DO - 10.1016/j.jlumin.2016.02.032
M3 - Article
AN - SCOPUS:84960110654
VL - 175
SP - 122
EP - 128
JO - Journal of Luminescence
JF - Journal of Luminescence
SN - 0022-2313
ER -