A new fluorescent chemosensor for highly selective and sensitive detection of inorganic phosphate (Pi) in aqueous solution and living cells

Qingtao Meng*, Yue Wang, Ming Yang, Run Zhang, Renjie Wang, Zhiqiang Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

In this work, a new fluorescence chemosensor, FP-Fe3+ was developed for the detection of Pi in aqueous solution and living cells. The unique ligand, FP displayed a high affinity to Fe3+ (Ka = 1.40 × 106 M-1) in the presence of other competing cations, accompanied with a dramatic fluorescence quenching. The specific interaction of Pi and FP-Fe3+ ensemble led to the liberation of fluorophore, FP, and thus the fluorescence was recovered. The dose-dependent fluorescence enhancement of FP-Fe3+ showed a good linearity with a detection limit of 300 nM for Pi. The extraordinary performance of the present chemosensor, including high sensitivity, selectivity, and good biocompatibility enable the investigation of fluorescent response for Pi in living cells by confocal microscope. Quantitative monitoring of intracellular Pi was achieved by the flow cytometry analysis.

Original languageEnglish
Pages (from-to)53189-53197
Number of pages9
JournalRSC Advances
Volume5
Issue number66
DOIs
Publication statusPublished - 2015

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