A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite

F. Guo, X. X. Xu, Z. Z. Sun, J. X. Zhang, Z. X. Meng, W. Zheng*, H. M. Zhou, B. L. Wang, Y. F. Zheng

*Corresponding author for this work

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (ks) was calculated to be 270.6s-1. The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H2O2. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 5×10-6molL-1 to 30×10-6molL-1, with a detection limit of 0.37×10-6molL-1 (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (Kmapp) was 77.7μmolL-1. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume86
Issue number1
DOIs
Publication statusPublished - 1 Aug 2011
Externally publishedYes

Keywords

  • Biosensor
  • Collagen
  • Direct electron transfer
  • Electrocatalysis
  • Electrospinning
  • Hemoglobin

Fingerprint Dive into the research topics of 'A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite'. Together they form a unique fingerprint.

  • Cite this