A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite

F. Guo; X. X. Xu; Z. Z. Sun; J. X. Zhang; Z. X. Meng; W. Zheng; H. M. Zhou; B. L. Wang; Y. F. Zheng

doi:10.1016/j.colsurfb.2011.03.032

A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite

F. Guo, X. X. Xu, Z. Z. Sun, J. X. Zhang, Z. X. Meng, W. Zheng^*, H. M. Zhou, B. L. Wang, Y. F. Zheng

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (k_s) was calculated to be 270.6s^-1. The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H₂O₂. The amperometric response of the biosensor varied linearly with the H₂O₂ concentration ranging from 5×10^-6molL^-1 to 30×10^-6molL^-1, with a detection limit of 0.37×10^-6molL^-1 (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (Kmapp) was 77.7μmolL^-1. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability.

Original language	English
Pages (from-to)	140-145
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	86
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfb.2011.03.032
Publication status	Published - 1 Aug 2011
Externally published	Yes

Keywords

Biosensor
Collagen
Direct electron transfer
Electrocatalysis
Electrospinning
Hemoglobin

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10.1016/j.colsurfb.2011.03.032

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title = "A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite",

abstract = "In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (ks) was calculated to be 270.6s-1. The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H2O2. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 5×10-6molL-1 to 30×10-6molL-1, with a detection limit of 0.37×10-6molL-1 (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (Kmapp) was 77.7μmolL-1. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability.",

keywords = "Biosensor, Collagen, Direct electron transfer, Electrocatalysis, Electrospinning, Hemoglobin",

author = "F. Guo and Xu, {X. X.} and Sun, {Z. Z.} and Zhang, {J. X.} and Meng, {Z. X.} and W. Zheng and Zhou, {H. M.} and Wang, {B. L.} and Zheng, {Y. F.}",

year = "2011",

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doi = "10.1016/j.colsurfb.2011.03.032",

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volume = "86",

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journal = "Colloids and Surfaces B: Biointerfaces",

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AU - Guo, F.

AU - Xu, X. X.

AU - Sun, Z. Z.

AU - Zhang, J. X.

AU - Meng, Z. X.

AU - Zheng, W.

AU - Zhou, H. M.

AU - Wang, B. L.

AU - Zheng, Y. F.

PY - 2011/8/1

Y1 - 2011/8/1

N2 - In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (ks) was calculated to be 270.6s-1. The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H2O2. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 5×10-6molL-1 to 30×10-6molL-1, with a detection limit of 0.37×10-6molL-1 (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (Kmapp) was 77.7μmolL-1. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability.

AB - In this paper, the hemoglobin (Hb)-collagen microbelt modified electrode with three-dimensional configuration was fabricated via the electrospinning method. Direct electron transfer of the Hb immobilized into the electrospun collagen microbelts was greatly facilitated. The apparent heterogeneous electron transfer rate constant (ks) was calculated to be 270.6s-1. The electrospun Hb-collagen microbelt modified electrode showed an excellent bioelectrocatalytic activity toward the reduction of H2O2. The amperometric response of the biosensor varied linearly with the H2O2 concentration ranging from 5×10-6molL-1 to 30×10-6molL-1, with a detection limit of 0.37×10-6molL-1 (signal-to-noise ratio of 3). The apparent Michaelis-Menten constant (Kmapp) was 77.7μmolL-1. The established biosensor exhibited fast amperometric response, high sensitivity, good reproducibility and stability.

KW - Biosensor

KW - Collagen

KW - Direct electron transfer

KW - Electrocatalysis

KW - Electrospinning

KW - Hemoglobin

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

IS - 1

ER -

A novel amperometric hydrogen peroxide biosensor based on electrospun Hb-collagen composite

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Keywords

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