A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite

X. X. Xu; J. X. Zhang; F. Guo; W. Zheng; H. M. Zhou; B. L. Wang; Y. F. Zheng; Y. B. Wang; Y. Cheng; X. Lou; B. Z. Jang

doi:10.1016/j.colsurfb.2011.01.037

A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite

X. X. Xu, J. X. Zhang, F. Guo, W. Zheng^*, H. M. Zhou, B. L. Wang, Y. F. Zheng, Y. B. Wang, Y. Cheng, X. Lou, B. Z. Jang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

In this paper, an amperometric biosensor of hydrogen peroxide (H₂O₂) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ) and apparent heterogeneous electron transfer rate constant (k_s) were calculated to be (1.60±0.17)×10^-10molcm^-2 and 48.51s^-1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H₂O₂. The biosensor of H₂O₂ exhibited a linear response to H₂O₂ in the range of 10-150μM and a detection limit of 8.24μM (S/N=3) was obtained. The apparent Michaelis-Menten constant (Kmapp) was 45.35μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness.

Original language	English
Pages (from-to)	427-432
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	84
Issue number	2
DOIs	https://doi.org/10.1016/j.colsurfb.2011.01.037
Publication status	Published - 1 Jun 2011
Externally published	Yes

Keywords

Biosensor
Direct electron transfer
Electrocatalysis
Hemoglobin
Nanographene platelets

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10.1016/j.colsurfb.2011.01.037

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title = "A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite",

abstract = "In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60±0.17)×10-10molcm-2 and 48.51s-1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10-150μM and a detection limit of 8.24μM (S/N=3) was obtained. The apparent Michaelis-Menten constant (Kmapp) was 45.35μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness.",

keywords = "Biosensor, Direct electron transfer, Electrocatalysis, Hemoglobin, Nanographene platelets",

author = "Xu, {X. X.} and Zhang, {J. X.} and F. Guo and W. Zheng and Zhou, {H. M.} and Wang, {B. L.} and Zheng, {Y. F.} and Wang, {Y. B.} and Y. Cheng and X. Lou and Jang, {B. Z.}",

year = "2011",

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doi = "10.1016/j.colsurfb.2011.01.037",

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AU - Xu, X. X.

AU - Zhang, J. X.

AU - Guo, F.

AU - Zheng, W.

AU - Zhou, H. M.

AU - Wang, B. L.

AU - Zheng, Y. F.

AU - Wang, Y. B.

AU - Cheng, Y.

AU - Lou, X.

AU - Jang, B. Z.

PY - 2011/6/1

Y1 - 2011/6/1

N2 - In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60±0.17)×10-10molcm-2 and 48.51s-1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10-150μM and a detection limit of 8.24μM (S/N=3) was obtained. The apparent Michaelis-Menten constant (Kmapp) was 45.35μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness.

AB - In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60±0.17)×10-10molcm-2 and 48.51s-1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10-150μM and a detection limit of 8.24μM (S/N=3) was obtained. The apparent Michaelis-Menten constant (Kmapp) was 45.35μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness.

KW - Biosensor

KW - Direct electron transfer

KW - Electrocatalysis

KW - Hemoglobin

KW - Nanographene platelets

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A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite

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