A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite

X. X. Xu, J. X. Zhang, F. Guo, W. Zheng*, H. M. Zhou, B. L. Wang, Y. F. Zheng, Y. B. Wang, Y. Cheng, X. Lou, B. Z. Jang

*Corresponding author for this work

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60±0.17)×10-10molcm-2 and 48.51s-1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10-150μM and a detection limit of 8.24μM (S/N=3) was obtained. The apparent Michaelis-Menten constant (Kmapp) was 45.35μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness.

Original languageEnglish
Pages (from-to)427-432
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume84
Issue number2
DOIs
Publication statusPublished - 1 Jun 2011
Externally publishedYes

Keywords

  • Biosensor
  • Direct electron transfer
  • Electrocatalysis
  • Hemoglobin
  • Nanographene platelets

Fingerprint Dive into the research topics of 'A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite'. Together they form a unique fingerprint.

  • Cite this