TY - JOUR
T1 - A novel assay detecting recall response to Mycobacterium tuberculosis
T2 - comparison with existing assays
AU - Hsu, Denise C.
AU - Zaunders, John J.
AU - Plit, Marshall
AU - Leeman, Craig
AU - Ip, Susanna
AU - Iampornsin, Thatri
AU - Pett, Sarah L.
AU - Bailey, Michelle
AU - Amin, Janaki
AU - Ubolyam, Sasiwimol
AU - Avihingsanon, Anchalee
AU - Ananworanich, Jintanat
AU - Ruxrungtham, Kiat
AU - Cooper, David A.
AU - Kelleher, Anthony D.
PY - 2012/7
Y1 - 2012/7
N2 - A strategy to reduce the burden of active TB is isoniazid preventive therapy for latent TB infection (LTBI). However, current assays used to diagnose LTBI all have limitations. In these proof of concept studies, we compared the agreement of a novel flow cytometry assay detecting CD25/CD134 co-expression with QuantiFERON-TB Gold In-Tube (QFN-GIT) and Tuberculin skin test (TST) in the detection of recall immune response to TB. The CD25/CD134 assay, QFN-GIT and TST were performed on 74 participants referred for TB screening in Sydney and on 50 participants with advanced HIV infection (CD4 ≤ 350 × 106 cells/L) in Bangkok. The agreement between CD25/CD134 assay and QFN-GIT was 93.2% (Kappa 0.631 95% CI 0.336-0.926) in Sydney and 90% (Kappa 0.747 95% CI 0.541-0.954) in Bangkok. Discordant results occurred around the cut off of both tests. The agreement between CD25/CD134 assay and TST was 73.6% (Kappa 0.206 95% CI 0.004-0.409) in Sydney and 84% (Kappa 0.551 95% CI 0.296-0.806) in Bangkok. The CD25/CD134 assay showed good agreement with QFN-GIT in detecting recall response to TB both in well and less resourced setting as well as in persons with advanced HIV infection. Further study into the performance of this assay is thus warranted.
AB - A strategy to reduce the burden of active TB is isoniazid preventive therapy for latent TB infection (LTBI). However, current assays used to diagnose LTBI all have limitations. In these proof of concept studies, we compared the agreement of a novel flow cytometry assay detecting CD25/CD134 co-expression with QuantiFERON-TB Gold In-Tube (QFN-GIT) and Tuberculin skin test (TST) in the detection of recall immune response to TB. The CD25/CD134 assay, QFN-GIT and TST were performed on 74 participants referred for TB screening in Sydney and on 50 participants with advanced HIV infection (CD4 ≤ 350 × 106 cells/L) in Bangkok. The agreement between CD25/CD134 assay and QFN-GIT was 93.2% (Kappa 0.631 95% CI 0.336-0.926) in Sydney and 90% (Kappa 0.747 95% CI 0.541-0.954) in Bangkok. Discordant results occurred around the cut off of both tests. The agreement between CD25/CD134 assay and TST was 73.6% (Kappa 0.206 95% CI 0.004-0.409) in Sydney and 84% (Kappa 0.551 95% CI 0.296-0.806) in Bangkok. The CD25/CD134 assay showed good agreement with QFN-GIT in detecting recall response to TB both in well and less resourced setting as well as in persons with advanced HIV infection. Further study into the performance of this assay is thus warranted.
KW - Antigen specific CD4, CD134, CD25
KW - Mycobacterium tuberculosis
KW - Quantiferon gold in tube
UR - http://www.scopus.com/inward/record.url?scp=84862002944&partnerID=8YFLogxK
U2 - 10.1016/j.tube.2012.03.008
DO - 10.1016/j.tube.2012.03.008
M3 - Article
C2 - 22542644
AN - SCOPUS:84862002944
SN - 1472-9792
VL - 92
SP - 321
EP - 327
JO - Tuberculosis
JF - Tuberculosis
IS - 4
ER -