A novel assay detecting recall response to Mycobacterium tuberculosis

comparison with existing assays

Denise C. Hsu*, John J. Zaunders, Marshall Plit, Craig Leeman, Susanna Ip, Thatri Iampornsin, Sarah L. Pett, Michelle Bailey, Janaki Amin, Sasiwimol Ubolyam, Anchalee Avihingsanon, Jintanat Ananworanich, Kiat Ruxrungtham, David A. Cooper, Anthony D. Kelleher

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A strategy to reduce the burden of active TB is isoniazid preventive therapy for latent TB infection (LTBI). However, current assays used to diagnose LTBI all have limitations. In these proof of concept studies, we compared the agreement of a novel flow cytometry assay detecting CD25/CD134 co-expression with QuantiFERON-TB Gold In-Tube (QFN-GIT) and Tuberculin skin test (TST) in the detection of recall immune response to TB. The CD25/CD134 assay, QFN-GIT and TST were performed on 74 participants referred for TB screening in Sydney and on 50 participants with advanced HIV infection (CD4 ≤ 350 × 106 cells/L) in Bangkok. The agreement between CD25/CD134 assay and QFN-GIT was 93.2% (Kappa 0.631 95% CI 0.336-0.926) in Sydney and 90% (Kappa 0.747 95% CI 0.541-0.954) in Bangkok. Discordant results occurred around the cut off of both tests. The agreement between CD25/CD134 assay and TST was 73.6% (Kappa 0.206 95% CI 0.004-0.409) in Sydney and 84% (Kappa 0.551 95% CI 0.296-0.806) in Bangkok. The CD25/CD134 assay showed good agreement with QFN-GIT in detecting recall response to TB both in well and less resourced setting as well as in persons with advanced HIV infection. Further study into the performance of this assay is thus warranted.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalTuberculosis
Volume92
Issue number4
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

Keywords

  • Antigen specific CD4, CD134, CD25
  • Mycobacterium tuberculosis
  • Quantiferon gold in tube

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    Hsu, D. C., Zaunders, J. J., Plit, M., Leeman, C., Ip, S., Iampornsin, T., ... Kelleher, A. D. (2012). A novel assay detecting recall response to Mycobacterium tuberculosis: comparison with existing assays. Tuberculosis, 92(4), 321-327. https://doi.org/10.1016/j.tube.2012.03.008