A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers

J. Li; H. Mei; W. Zheng; P. Pan; X. J. Sun; F. Li; F. Guo; H. M. Zhou; J. Y. Ma; X. X. Xu; Y. F. Zheng

doi:10.1016/j.colsurfb.2014.03.035

A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers

J. Li, H. Mei, W. Zheng^*, P. Pan, X. J. Sun, F. Li, F. Guo, H. M. Zhou, J. Y. Ma, X. X. Xu, Y. F. Zheng

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (k_s) of the direct electron transfer between Hb and electrodes was 5.3s^-1. In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H₂O₂ with a detection limit of 0.91μM (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (Kmapp) of 32.6μM.

Original language	English
Pages (from-to)	77-82
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	118
DOIs	https://doi.org/10.1016/j.colsurfb.2014.03.035
Publication status	Published - 1 Jun 2014
Externally published	Yes

Keywords

Biosensor
Electrochemistry
Electrospinning
Hemoglobin-collagen-CNTs nanofibers

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10.1016/j.colsurfb.2014.03.035

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@article{4e0d53bc544349a9ba46013b936af1fd,

title = "A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers",

abstract = "In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (ks) of the direct electron transfer between Hb and electrodes was 5.3s-1. In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H2O2 with a detection limit of 0.91μM (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (Kmapp) of 32.6μM.",

keywords = "Biosensor, Electrochemistry, Electrospinning, Hemoglobin-collagen-CNTs nanofibers",

author = "J. Li and H. Mei and W. Zheng and P. Pan and Sun, {X. J.} and F. Li and F. Guo and Zhou, {H. M.} and Ma, {J. Y.} and Xu, {X. X.} and Zheng, {Y. F.}",

year = "2014",

month = jun,

day = "1",

doi = "10.1016/j.colsurfb.2014.03.035",

language = "English",

volume = "118",

pages = "77--82",

journal = "Colloids and Surfaces B: Biointerfaces",

issn = "0927-7765",

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TY - JOUR

T1 - A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers

AU - Li, J.

AU - Mei, H.

AU - Zheng, W.

AU - Pan, P.

AU - Sun, X. J.

AU - Li, F.

AU - Guo, F.

AU - Zhou, H. M.

AU - Ma, J. Y.

AU - Xu, X. X.

AU - Zheng, Y. F.

PY - 2014/6/1

Y1 - 2014/6/1

N2 - In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (ks) of the direct electron transfer between Hb and electrodes was 5.3s-1. In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H2O2 with a detection limit of 0.91μM (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (Kmapp) of 32.6μM.

AB - In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (ks) of the direct electron transfer between Hb and electrodes was 5.3s-1. In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H2O2 with a detection limit of 0.91μM (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (Kmapp) of 32.6μM.

KW - Biosensor

KW - Electrochemistry

KW - Electrospinning

KW - Hemoglobin-collagen-CNTs nanofibers

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U2 - 10.1016/j.colsurfb.2014.03.035

DO - 10.1016/j.colsurfb.2014.03.035

M3 - Article

C2 - 24732396

AN - SCOPUS:84922392084

SN - 0927-7765

VL - 118

SP - 77

EP - 82

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers

Abstract

Keywords

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