A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers

J. Li, H. Mei, W. Zheng*, P. Pan, X. J. Sun, F. Li, F. Guo, H. M. Zhou, J. Y. Ma, X. X. Xu, Y. F. Zheng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (ks) of the direct electron transfer between Hb and electrodes was 5.3s-1. In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H2O2 with a detection limit of 0.91μM (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (Kmapp) of 32.6μM.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Publication statusPublished - 1 Jun 2014
Externally publishedYes


  • Biosensor
  • Electrochemistry
  • Electrospinning
  • Hemoglobin-collagen-CNTs nanofibers


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