A novel method for marking microinjection sites using methylene blue and diaminobenzidine

Todd A. Verner; Ann K. Goodchild; Paul M. Pilowsky

doi:10.1016/S0165-0270(03)00018-9

A novel method for marking microinjection sites using methylene blue and diaminobenzidine

Todd A. Verner, Ann K. Goodchild, Paul M. Pilowsky^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A number of different compounds have been microinjected into the brains of animals for the purpose of marking injection sites. In the present study, we describe a novel method for visualising and permanently preserving sites in the brainstem of rats marked by microinjections of methylene blue. Subsequent exposure of the sections to diaminobenzidine (DAB) tetrahydrochloride in the presence of glucose oxidase or infrared light produced an insoluble black precipitate at the injection site. A major advantage of this method is that the injection sites are marked simultaneously with immunohistochemical processing. Crown

Original language	English
Pages (from-to)	207-211
Number of pages	5
Journal	Journal of Neuroscience Methods
Volume	124
Issue number	2
DOIs	https://doi.org/10.1016/S0165-0270(03)00018-9
Publication status	Published - 15 Apr 2003
Externally published	Yes

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10.1016/S0165-0270(03)00018-9

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abstract = "A number of different compounds have been microinjected into the brains of animals for the purpose of marking injection sites. In the present study, we describe a novel method for visualising and permanently preserving sites in the brainstem of rats marked by microinjections of methylene blue. Subsequent exposure of the sections to diaminobenzidine (DAB) tetrahydrochloride in the presence of glucose oxidase or infrared light produced an insoluble black precipitate at the injection site. A major advantage of this method is that the injection sites are marked simultaneously with immunohistochemical processing. Crown",

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AU - Goodchild, Ann K.

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AB - A number of different compounds have been microinjected into the brains of animals for the purpose of marking injection sites. In the present study, we describe a novel method for visualising and permanently preserving sites in the brainstem of rats marked by microinjections of methylene blue. Subsequent exposure of the sections to diaminobenzidine (DAB) tetrahydrochloride in the presence of glucose oxidase or infrared light produced an insoluble black precipitate at the injection site. A major advantage of this method is that the injection sites are marked simultaneously with immunohistochemical processing. Crown

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A novel method for marking microinjection sites using methylene blue and diaminobenzidine

Abstract

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