TY - JOUR
T1 - A novel microfluidic device-vased neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons
AU - Stefen, Holly
AU - Hassanzadeh-Barforoushi, Amin
AU - Brettle, Merryn
AU - Fok, Sandra
AU - Suchowerska, Alexandra K.
AU - Tedla, Nicodemus
AU - Barber, Tracie
AU - Warkiani, Majid Ebrahimi
AU - Fath, Thomas
PY - 2018/11
Y1 - 2018/11
N2 - Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system.
AB - Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system.
KW - Microfluidic systems
KW - Neurite outgrowth inhibition
KW - NogoA
KW - Tropomyosins
UR - http://www.scopus.com/inward/record.url?scp=85053443919&partnerID=8YFLogxK
U2 - 10.1007/s10571-018-0620-7
DO - 10.1007/s10571-018-0620-7
M3 - Article
C2 - 30218404
AN - SCOPUS:85053443919
VL - 38
SP - 1557
EP - 1563
JO - Cellular and Molecular Neurobiology
JF - Cellular and Molecular Neurobiology
SN - 0272-4340
IS - 8
ER -