A novel microfluidic device-based neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons

Holly Stefen; Amin Hassanzadeh-Barforoushi; Merryn Brettle; Sandra Fok; Alexandra K. Suchowerska; Nicodemus Tedla; Tracie Barber; Majid Ebrahimi  Warkiani; Thomas Fath

doi:10.1007/s10571-018-0620-7

A novel microfluidic device-based neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons

Holly Stefen, Amin Hassanzadeh-Barforoushi, Merryn Brettle, Sandra Fok, Alexandra K. Suchowerska, Nicodemus Tedla, Tracie Barber, Majid Ebrahimi Warkiani, Thomas Fath^*

^*Corresponding author for this work

Dementia Research Centre

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system.

Original language	English
Pages (from-to)	1557-1563
Number of pages	7
Journal	Cellular and Molecular Neurobiology
Volume	38
Issue number	8
Early online date	14 Sept 2018
DOIs	https://doi.org/10.1007/s10571-018-0620-7
Publication status	Published - Nov 2018

Keywords

Microfluidic systems
Neurite outgrowth inhibition
NogoA
Tropomyosins

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10.1007/s10571-018-0620-7

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Stefen, H., Hassanzadeh-Barforoushi, A., Brettle, M., Fok, S., Suchowerska, A. K., Tedla, N., Barber, T., Warkiani, M. E., & Fath, T. (2018). A novel microfluidic device-based neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons. Cellular and Molecular Neurobiology, 38(8), 1557-1563. https://doi.org/10.1007/s10571-018-0620-7

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title = "A novel microfluidic device-based neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons",

abstract = "Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system.",

keywords = "Microfluidic systems, Neurite outgrowth inhibition, NogoA, Tropomyosins",

author = "Holly Stefen and Amin Hassanzadeh-Barforoushi and Merryn Brettle and Sandra Fok and Suchowerska, {Alexandra K.} and Nicodemus Tedla and Tracie Barber and Warkiani, {Majid Ebrahimi} and Thomas Fath",

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Stefen, H, Hassanzadeh-Barforoushi, A, Brettle, M, Fok, S, Suchowerska, AK, Tedla, N, Barber, T, Warkiani, ME & Fath, T 2018, 'A novel microfluidic device-based neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons', Cellular and Molecular Neurobiology, vol. 38, no. 8, pp. 1557-1563. https://doi.org/10.1007/s10571-018-0620-7

A novel microfluidic device-based neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons. / Stefen, Holly; Hassanzadeh-Barforoushi, Amin; Brettle, Merryn et al.
In: Cellular and Molecular Neurobiology, Vol. 38, No. 8, 11.2018, p. 1557-1563.

Research output: Contribution to journal › Article › peer-review

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AU - Stefen, Holly

AU - Hassanzadeh-Barforoushi, Amin

AU - Brettle, Merryn

AU - Fok, Sandra

AU - Suchowerska, Alexandra K.

AU - Tedla, Nicodemus

AU - Barber, Tracie

AU - Warkiani, Majid Ebrahimi

AU - Fath, Thomas

PY - 2018/11

Y1 - 2018/11

N2 - Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system.

AB - Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system.

KW - Microfluidic systems

KW - Neurite outgrowth inhibition

KW - NogoA

KW - Tropomyosins

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SN - 0272-4340

VL - 38

SP - 1557

EP - 1563

JO - Cellular and Molecular Neurobiology

JF - Cellular and Molecular Neurobiology

IS - 8

ER -

A novel microfluidic device-based neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons

Abstract

Keywords

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Complete blood fractionation using a low-cost microfluidic system

NBR: Defining the spatial and temporal regulation of neurite branching

Targeting the synaptic actin cytoskeleton in Alzheimer's Disease

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A novel microfluidic device-based neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

Complete blood fractionation using a low-cost microfluidic system

NBR: Defining the spatial and temporal regulation of neurite branching

Targeting the synaptic actin cytoskeleton in Alzheimer's Disease

Cite this