Projects per year
Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system.
|Number of pages||7|
|Journal||Cellular and Molecular Neurobiology|
|Early online date||14 Sep 2018|
|Publication status||Published - Nov 2018|
- Microfluidic systems
- Neurite outgrowth inhibition
FingerprintDive into the research topics of 'A novel microfluidic device-based neurite outgrowth inhibition assay reveals the neurite outgrowth-promoting activity of tropomyosin Tpm3.1 in hippocampal neurons'. Together they form a unique fingerprint.
Complete blood fractionation using a low-cost microfluidic system
Inglis, D., Ebrahimi Warkiani, M., Chan, M. & Karlin, I.
15/02/17 → …
NBR: Defining the spatial and temporal regulation of neurite branching
Fath, T., Gunning, P., Brandt, R., Klotzsch, E., Karl, T. & Delerue, F.
18/12/18 → 17/12/21
Targeting the synaptic actin cytoskeleton in Alzheimer's Disease
Fath, T., Anggono, V., Gunning, P., Power, J., Karl, T. & Masedunskas, A.
1/01/15 → 31/12/18