A novel microfluidic patterning device for neuron-glia co-culture

A. Shi; J. Shemesh; M. Asadnia; U. A. Robles; R. Green; G. H. Yeoh; M. E. Warkiani

A novel microfluidic patterning device for neuron-glia co-culture

A. Shi, J. Shemesh, M. Asadnia, U. A. Robles, R. Green, G. H. Yeoh, M. E. Warkiani^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

Many biological processes in the body are regulated by synchronized activity between two cell types. To study cell-cell interactions, it is necessary to develop easy to use co-culture systems, where different cell types can be cultured within the same confined space. Designing a complete 3D biomimetic system to study these interactions in vitro requires complex protocols and use of non-conventional materials such as hydrogels. This paper reports development of a temporarily sealed microfluidic device which utilizes a novel valve design to directly and accurately co-culture two cell lines in alternating rows, allowing them to proliferate towards each other and then observe their interaction at the boundaries of their interface.

Original language	English
Title of host publication	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
Place of Publication	Gyeongju, South Korea
Publisher	Chemical and Biological Microsystems Society
Pages	633-635
Number of pages	3
ISBN (Electronic)	9780979806483
ISBN (Print)	9781510817876
Publication status	Published - 2015
Externally published	Yes
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015

Other

Other	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country/Territory	Korea, Republic of
City	Gyeongju
Period	25/10/15 → 29/10/15

Keywords

Co-culture
Microfluidics
Neurons

Cite this

@inproceedings{2309b4b1557f455c83ddf01a49c1e08f,

title = "A novel microfluidic patterning device for neuron-glia co-culture",

abstract = "Many biological processes in the body are regulated by synchronized activity between two cell types. To study cell-cell interactions, it is necessary to develop easy to use co-culture systems, where different cell types can be cultured within the same confined space. Designing a complete 3D biomimetic system to study these interactions in vitro requires complex protocols and use of non-conventional materials such as hydrogels. This paper reports development of a temporarily sealed microfluidic device which utilizes a novel valve design to directly and accurately co-culture two cell lines in alternating rows, allowing them to proliferate towards each other and then observe their interaction at the boundaries of their interface.",

keywords = "Co-culture, Microfluidics, Neurons",

author = "A. Shi and J. Shemesh and M. Asadnia and Robles, {U. A.} and R. Green and Yeoh, {G. H.} and Warkiani, {M. E.}",

year = "2015",

language = "English",

isbn = "9781510817876",

pages = "633--635",

booktitle = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)",

publisher = "Chemical and Biological Microsystems Society",

note = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 ; Conference date: 25-10-2015 Through 29-10-2015",

}

Shi, A, Shemesh, J, Asadnia, M, Robles, UA, Green, R, Yeoh, GH & Warkiani, ME 2015, A novel microfluidic patterning device for neuron-glia co-culture. in 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). Chemical and Biological Microsystems Society, Gyeongju, South Korea, pp. 633-635, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 25/10/15.

A novel microfluidic patterning device for neuron-glia co-culture. / Shi, A.; Shemesh, J.; Asadnia, M. et al.

19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). Gyeongju, South Korea : Chemical and Biological Microsystems Society, 2015. p. 633-635.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - A novel microfluidic patterning device for neuron-glia co-culture

AU - Shi, A.

AU - Shemesh, J.

AU - Asadnia, M.

AU - Robles, U. A.

AU - Green, R.

AU - Yeoh, G. H.

AU - Warkiani, M. E.

PY - 2015

Y1 - 2015

N2 - Many biological processes in the body are regulated by synchronized activity between two cell types. To study cell-cell interactions, it is necessary to develop easy to use co-culture systems, where different cell types can be cultured within the same confined space. Designing a complete 3D biomimetic system to study these interactions in vitro requires complex protocols and use of non-conventional materials such as hydrogels. This paper reports development of a temporarily sealed microfluidic device which utilizes a novel valve design to directly and accurately co-culture two cell lines in alternating rows, allowing them to proliferate towards each other and then observe their interaction at the boundaries of their interface.

AB - Many biological processes in the body are regulated by synchronized activity between two cell types. To study cell-cell interactions, it is necessary to develop easy to use co-culture systems, where different cell types can be cultured within the same confined space. Designing a complete 3D biomimetic system to study these interactions in vitro requires complex protocols and use of non-conventional materials such as hydrogels. This paper reports development of a temporarily sealed microfluidic device which utilizes a novel valve design to directly and accurately co-culture two cell lines in alternating rows, allowing them to proliferate towards each other and then observe their interaction at the boundaries of their interface.

KW - Co-culture

KW - Microfluidics

KW - Neurons

UR - http://www.scopus.com/inward/record.url?scp=84983320493&partnerID=8YFLogxK

M3 - Conference proceeding contribution

AN - SCOPUS:84983320493

SN - 9781510817876

SP - 633

EP - 635

BT - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)

PB - Chemical and Biological Microsystems Society

CY - Gyeongju, South Korea

T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015

Y2 - 25 October 2015 through 29 October 2015

ER -

A novel microfluidic patterning device for neuron-glia co-culture

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